Detailed information NTG0156030

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: RN80_RS07145
• Genome accession: NZ_CP012646
• Coordinates: 1466857..1467495 (-)
• Length: 212 a.a.
• NCBI ID: WP_060628475.1
• Uniprot ID: -
• Organism: Streptococcus mitis strain KCOM 1350 (= ChDC B183)
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1461857..1472495

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RN80_RS07125 (RN80_07140)	-	1462459..1463643 (-)	1185	WP_060628469.1	hypothetical protein	-
RN80_RS07130 (RN80_07145)	-	1463647..1464234 (-)	588	WP_060628471.1	ATP-binding cassette domain-containing protein	-
RN80_RS07140 (RN80_07155)	comEC/celB	1464633..1466873 (-)	2241	WP_060628473.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RN80_RS07145 (RN80_07160)	comEA/celA/cilE	1466857..1467495 (-)	639	WP_060628475.1	helix-hairpin-helix domain-containing protein	Machinery gene
RN80_RS07150 (RN80_07165)	-	1467562..1468131 (-)	570	WP_060628477.1	GNAT family N-acetyltransferase	-
RN80_RS07155 (RN80_07170)	-	1468238..1469242 (-)	1005	WP_060628478.1	PhoH family protein	-
RN80_RS07160 (RN80_07175)	-	1469323..1469538 (-)	216	WP_001232084.1	YozE family protein	-
RN80_RS07165 (RN80_07180)	-	1469584..1470141 (-)	558	WP_004234343.1	GrpB family protein	-
RN80_RS07170 (RN80_07185)	cvfB	1470153..1471007 (-)	855	WP_060628479.1	RNA-binding virulence regulatory protein CvfB	-
RN80_RS07175 (RN80_07190)	frr	1471067..1471624 (-)	558	WP_060628480.1	ribosome recycling factor	-
RN80_RS07180 (RN80_07195)	pyrH	1471633..1472370 (-)	738	WP_000002997.1	UMP kinase	-

Sequence

Protein

• Length: 212 a.a.
• Molecular weight: 22649.83 Da
• Isoelectric Point: 6.5036

>NTDB_id=156030 RN80_RS07145 WP_060628475.1 1466857..1467495(-) (comEA/celA/cilE) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDSSTEKEVKEEPVEQDLITVDVKGAVKS
PGIYDLSVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEESASQHTGSGAPSSTSKDKKINLNKASLEE
LKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 639 bp

>NTDB_id=156030 RN80_RS07145 WP_060628475.1 1466857..1467495(-) (comEA/celA/cilE) [Streptococcus mitis strain KCOM 1350 (= ChDC B183)]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGAGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTAAAAGAAACAAATTTACAGGCAGAAGTTGCAGCTGTTTCCAAGGATT
CATCGACCGAAAAGGAAGTGAAGGAAGAACCGGTTGAACAAGATCTAATCACAGTAGATGTCAAAGGTGCTGTAAAATCA
CCAGGGATTTATGATTTGTCTGTAGGTAGTCGGGTCAATGACGCTGTTCAAAAGGCGGGTGGCTTGACAGAGCAAGCAGA
CAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAAGCTCTGGTTTACGTTCCCACTAAGGGAGAAGAATCAGCTA
GTCAACATACTGGTTCTGGAGCGCCTTCTTCAACAAGCAAGGACAAGAAGATCAATCTCAACAAAGCCAGTCTGGAAGAA
CTCAAGCAGGTCAAAGGACTGGGTGGCAAACGAGCTCAGGACATTATCGATCATCGTGAGGCGAATGGGAAGTTCAAGTC
GGTCGATGAACTCAAGAAGGTCTCTGGCATTGGCGCCAAAACGATAGAAAAGTTAAAAGACTATGTTACAGTGGACTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (72-122)

• Helix-hairpin-helix motif (HHH_3) (148-210)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus mitis SK321	95.833	100	0.976
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	93.056	100	0.948
comEA/celA/cilE	Streptococcus mitis NCTC 12261	91.667	100	0.934
comEA/celA/cilE	Streptococcus pneumoniae D39	90.278	100	0.92
comEA/celA/cilE	Streptococcus pneumoniae Rx1	90.278	100	0.92
comEA/celA/cilE	Streptococcus pneumoniae R6	90.278	100	0.92
comEA	Lactococcus lactis subsp. cremoris KW2	42.081	100	0.439
comEA	Latilactobacillus sakei subsp. sakei 23K	34.348	100	0.373
comEA	Streptococcus thermophilus LMD-9	52.027	69.811	0.363