Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AMQ28_RS06615 Genome accession   NZ_CP012608
Coordinates   1401441..1403153 (+) Length   570 a.a.
NCBI ID   WP_053578933.1    Uniprot ID   A0A1C4GWE4
Organism   Acinetobacter sp. TTH0-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1396441..1408153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMQ28_RS06575 (AMQ28_06580) nusA 1397194..1398678 (-) 1485 WP_053578929.1 transcription termination factor NusA -
  AMQ28_RS06580 (AMQ28_06585) rimP 1398718..1399242 (-) 525 WP_053578930.1 ribosome maturation factor RimP -
  AMQ28_RS06605 (AMQ28_06610) secG 1400026..1400355 (-) 330 WP_053578931.1 preprotein translocase subunit SecG -
  AMQ28_RS06610 (AMQ28_06615) tpiA 1400369..1401160 (-) 792 WP_053578932.1 triose-phosphate isomerase -
  AMQ28_RS06615 (AMQ28_06620) pilB 1401441..1403153 (+) 1713 WP_053578933.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AMQ28_RS06620 (AMQ28_06625) pilC 1403183..1404409 (+) 1227 WP_053578934.1 type II secretion system F family protein Machinery gene
  AMQ28_RS06625 (AMQ28_06630) pilD 1404409..1405269 (+) 861 WP_053578935.1 prepilin peptidase Machinery gene
  AMQ28_RS06630 (AMQ28_06635) coaE 1405283..1405873 (+) 591 WP_053578936.1 dephospho-CoA kinase -
  AMQ28_RS06635 (AMQ28_06640) - 1405890..1406810 (-) 921 WP_053578937.1 DMT family transporter -
  AMQ28_RS06640 (AMQ28_06645) rlmB 1406848..1407597 (-) 750 WP_053578938.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AMQ28_RS06645 (AMQ28_06650) - 1407729..1408052 (-) 324 WP_053578939.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63667.34 Da        Isoelectric Point: 5.0243

>NTDB_id=155750 AMQ28_RS06615 WP_053578933.1 1401441..1403153(+) (pilB) [Acinetobacter sp. TTH0-4]
MAALQSSPKFTGFIRRLVEDEIVSAENMQNAILSAKKAHQDIVPYLIDQLKIPALQIAEKISQEFGEPLFDLSIYDPAQI
IREGIDEKIITKHRILPIFKRGNLLYIATSNPTNMDAMDAIRFSSKMNVEAIIVEYPKLQKLIEQNFAEADTFEFSDDDI
DLNLEQEDISQKEDEDDDQKADEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLATRLAS
RLKVMSQMDISEKRLPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNAKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKVPANIPEPSLLEMGF
TEADLKHPDFHVYQPAGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASLKSGFNNLRRSGLMKVMQGITS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=155750 AMQ28_RS06615 WP_053578933.1 1401441..1403153(+) (pilB) [Acinetobacter sp. TTH0-4]
ATGGCAGCACTACAGAGTTCACCAAAATTTACGGGATTTATTCGACGTTTAGTTGAGGATGAGATCGTTTCTGCTGAAAA
CATGCAGAATGCAATATTAAGTGCAAAAAAAGCCCATCAAGATATTGTTCCTTATTTAATTGATCAGTTAAAAATACCGG
CCTTACAAATTGCAGAAAAAATTTCTCAGGAATTTGGTGAACCTCTATTCGACTTGAGCATTTATGATCCAGCCCAGATT
ATTCGTGAAGGAATTGATGAGAAAATTATAACCAAACATCGCATTCTTCCAATTTTTAAACGCGGCAATTTACTATATAT
CGCCACCAGCAATCCCACCAATATGGATGCCATGGACGCGATTCGTTTTAGCAGTAAAATGAATGTTGAAGCCATTATTG
TTGAATACCCAAAATTACAAAAACTCATTGAACAAAATTTTGCTGAAGCAGATACCTTTGAATTTTCAGATGATGATATA
GATCTTAATCTTGAACAGGAGGATATTAGCCAAAAAGAGGATGAAGATGATGACCAGAAGGCAGATGAAGCACCGATTGT
AAAATATATTAATAAGTTACTGATTGATGCAATTCGTATGGGTGCGTCAGATTTACATTTTGAACCGTATGAAAAAATGT
ATCGTGTGCGTTATCGTGTGGATGGTGTATTAAGACAAATAGCCAATCCACCGCTGCAACTTGCTACACGGTTGGCTTCG
CGTTTAAAAGTCATGTCGCAAATGGATATTTCAGAAAAACGCTTACCTCAAGATGGGCGAATTAAACTTAAACTTTCGAA
AAGTAAAGCCATTGATTTTCGTGTGAATTCACTCCCTACTCTATTTGGTGAAAAATTAGTTCTGCGTATTCTCGATCCTT
CAAGTGCCATGCTTGGAATTGACGCTTTAGGTTATGAGCCAGAGCAAAAAGAATTGTTCATGCAAGCACTAGATAAACCT
CAAGGCATGTTATTGATTACAGGACCTACGGGTTCAGGTAAAACGGTATCTTTATATACAGGTTTGAATATTCTGAACCG
TGAAGATACCAATATTTCAACTGCCGAAGATCCTGTGGAAATTAACTTGGAAGGGATTAATCAGGTTAATGTAAATGCAA
AAGTGGGATTAACATTCTCTGCTGCACTTAAATCTTTCTTGCGACAAGATCCTGATATTGTCATGGTCGGTGAGATTCGA
GATTTAGAAACGGCTGAAATTGCAATTAAAGCAGCGCAAACGGGTCATATGGTCATGTCCACATTACATACGAACAGTGC
ACCTGAAACCTTGACCCGACTCAGAAATATGGGGGTACCTTCCTTCAATATTGCAACCTCGGTAAACTTGGTGATTGCAC
AGCGTCTAGCTCGACGCTTATGTCCACAATGTAAAGTACCCGCGAATATTCCAGAACCTAGCTTATTAGAAATGGGCTTT
ACTGAAGCAGACCTCAAACATCCAGATTTCCATGTTTATCAACCAGCAGGCTGCTCAGAATGCCGAGAAGGCTACAAAGG
CCGTGTTGGGATTTATGAAGTAATGAAAGTAACACCTGAAATTTCAAAAATTATTATGGAAGATGGCAATGCACTTGAAA
TTGCTGCTGCATCTTTAAAATCGGGGTTTAATAATTTGCGTCGTTCAGGATTAATGAAAGTCATGCAAGGAATCACGTCA
CTTCAAGAAGTGAATCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C4GWE4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.474

100

0.795
  pilB Acinetobacter baylyi ADP1

76.316

100

0.763
  pilB Legionella pneumophila strain ERS1305867

53.731

94.035

0.505
  pilB Vibrio cholerae strain A1552

49.912

99.474

0.496
  pilF Neisseria gonorrhoeae MS11

48.932

98.596

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

51.644

90.702

0.468
  pilB Vibrio campbellii strain DS40M4

50.674

91.053

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

34.249

100

0.372
  pilF Thermus thermophilus HB27

37.117

97.368

0.361

Multiple sequence alignment