Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AK972_RS03370 Genome accession   NZ_CP012400
Coordinates   676947..678155 (-) Length   402 a.a.
NCBI ID   WP_063027058.1    Uniprot ID   -
Organism   Pseudomonas yamanorum strain LBUM636     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 671947..683155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AK972_RS03330 (AK972_0633) - 672829..673002 (-) 174 WP_010174986.1 DUF3094 family protein -
  AK972_RS03335 (AK972_0634) - 673092..673721 (-) 630 WP_008437312.1 DUF1780 domain-containing protein -
  AK972_RS03340 (AK972_0635) - 673824..674291 (+) 468 WP_063027052.1 hypothetical protein -
  AK972_RS03345 (AK972_0636) - 674288..674977 (+) 690 WP_063027054.1 energy-coupling factor ABC transporter permease -
  AK972_RS03350 (AK972_0637) - 675043..675258 (+) 216 WP_003215917.1 hypothetical protein -
  AK972_RS03355 (AK972_0638) yacG 675255..675455 (-) 201 WP_063027056.1 DNA gyrase inhibitor YacG -
  AK972_RS03360 (AK972_0639) coaE 675452..676075 (-) 624 WP_017477788.1 dephospho-CoA kinase -
  AK972_RS03365 (AK972_0640) pilD 676072..676950 (-) 879 WP_412458849.1 prepilin peptidase Machinery gene
  AK972_RS03370 (AK972_0641) pilC 676947..678155 (-) 1209 WP_063027058.1 type II secretion system F family protein Machinery gene
  AK972_RS03375 (AK972_0642) - 678357..678770 (+) 414 WP_063027060.1 pilin -
  AK972_RS03380 (AK972_0644) - 678857..680266 (+) 1410 WP_063027062.1 O-antigen ligase family protein -
  AK972_RS03390 (AK972_0645) - 680515..680820 (+) 306 WP_008437299.1 DUF6388 family protein -
  AK972_RS03395 (AK972_0646) nadC 680890..681738 (-) 849 WP_063027064.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43252.57 Da        Isoelectric Point: 10.0805

>NTDB_id=154296 AK972_RS03370 WP_063027058.1 676947..678155(-) (pilC) [Pseudomonas yamanorum strain LBUM636]
MNDVSAIYAWEGVNRKGRKVSGETSGHNPALIKALLRQQGINPGRVRKKSAGWPRLAPLVKPADIALFTRQLATLLKAGI
ALLQALDIIADGFENRHMRALVQSLKQDIAAGSSLAIALQKQPRYFDELYCNLIAAGEQAGALEALLERVATHLEKSEQL
KARIKKAMTYPMTVLAVATLVSAILLIHVVPQFQGLFAGVDAQLPGFTLGVISLSEFMQHNWWVLILVAGTGFAGLRKAY
RSSTGFRHWLDARLLKAPVAGTLLKKSAIARYARTLSTTFAAGVPLVQALDSVAGATGNGLFKQAINRMRHDVSTGTQLN
ESMAVSGLFPGMAIQMTAIGEESGTLDNMLEKVASHYEADVDNLVDNLTSLMEPLIMVVLGGIVGALVLAMYLPIFQLGT
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=154296 AK972_RS03370 WP_063027058.1 676947..678155(-) (pilC) [Pseudomonas yamanorum strain LBUM636]
ATGAATGATGTTTCAGCGATCTATGCCTGGGAAGGCGTCAACCGCAAAGGGCGCAAGGTATCTGGAGAAACCAGCGGTCA
CAATCCTGCATTGATCAAGGCACTGCTGCGCCAACAGGGCATTAATCCGGGCCGCGTACGCAAAAAATCCGCGGGTTGGC
CGAGACTCGCTCCGTTGGTCAAGCCTGCCGACATCGCCCTCTTCACCCGCCAGTTGGCAACGCTGTTGAAGGCCGGCATT
GCGCTTTTACAAGCACTGGATATCATCGCCGATGGCTTCGAAAACCGTCATATGCGGGCGCTGGTACAGAGCCTGAAACA
GGACATCGCCGCCGGCAGTAGTCTGGCTATCGCCCTGCAGAAACAGCCACGCTACTTCGATGAGCTGTACTGCAACCTGA
TTGCAGCGGGGGAGCAGGCGGGGGCACTCGAAGCGCTACTGGAACGAGTCGCCACCCACCTGGAAAAAAGCGAGCAACTG
AAAGCCAGAATCAAAAAGGCAATGACCTATCCCATGACGGTGCTGGCAGTCGCCACCCTTGTCAGTGCGATTTTGCTGAT
TCACGTTGTGCCACAGTTCCAGGGCCTGTTTGCCGGTGTGGATGCGCAGTTACCGGGCTTCACCCTGGGGGTCATCAGCT
TGTCGGAGTTCATGCAGCACAACTGGTGGGTACTGATTTTGGTCGCGGGTACCGGTTTCGCCGGACTGCGCAAAGCCTAT
CGCTCGTCAACAGGCTTTCGCCACTGGCTCGATGCCCGTTTGTTGAAAGCCCCGGTGGCAGGCACACTGCTGAAAAAATC
GGCGATCGCCCGCTACGCCCGCACGCTCTCGACCACCTTTGCAGCTGGCGTACCCTTGGTGCAAGCATTGGATTCCGTGG
CCGGCGCTACGGGTAATGGACTGTTCAAACAGGCAATCAATCGTATGCGGCACGACGTATCCACCGGGACACAACTGAAT
GAATCCATGGCCGTAAGCGGCCTGTTTCCAGGCATGGCGATCCAGATGACCGCGATCGGCGAGGAGTCGGGCACCCTCGA
CAACATGCTGGAAAAAGTAGCCAGCCACTACGAGGCCGACGTCGACAACCTGGTGGACAACCTCACCAGCCTGATGGAGC
CGCTGATCATGGTGGTACTCGGGGGTATCGTCGGGGCATTGGTGCTCGCCATGTACCTGCCGATCTTTCAACTCGGTACA
GCCTTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

60.202

98.756

0.595
  pilC Acinetobacter baylyi ADP1

54.637

99.254

0.542
  pilC Acinetobacter baumannii D1279779

52.152

98.259

0.512
  pilC Legionella pneumophila strain ERS1305867

49.62

98.259

0.488
  pilC Vibrio cholerae strain A1552

41.221

97.761

0.403
  pilG Neisseria gonorrhoeae MS11

40.102

98.01

0.393
  pilC Vibrio campbellii strain DS40M4

39.899

98.507

0.393
  pilG Neisseria meningitidis 44/76-A

39.34

98.01

0.386
  pilC Thermus thermophilus HB27

36.842

99.254

0.366

Multiple sequence alignment