Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AKN89_RS04985 Genome accession   NZ_CP012364
Coordinates   1060903..1062120 (-) Length   405 a.a.
NCBI ID   WP_053108119.1    Uniprot ID   -
Organism   Thiopseudomonas alkaliphila strain E1148     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1055903..1067120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AKN89_RS04960 (AKN89_04960) - 1056565..1057023 (+) 459 WP_053103346.1 YiiD C-terminal domain-containing protein -
  AKN89_RS04965 (AKN89_04965) - 1057020..1058231 (+) 1212 WP_053108116.1 NAD(P)/FAD-dependent oxidoreductase -
  AKN89_RS04970 (AKN89_04970) - 1058402..1059391 (+) 990 WP_053108117.1 hypothetical protein -
  AKN89_RS04975 (AKN89_04975) - 1059611..1059892 (+) 282 WP_053101006.1 GIY-YIG nuclease family protein -
  AKN89_RS04980 (AKN89_04980) pilD 1060003..1060899 (-) 897 WP_053108118.1 prepilin peptidase Machinery gene
  AKN89_RS04985 (AKN89_04985) pilC 1060903..1062120 (-) 1218 WP_053108119.1 type II secretion system F family protein Machinery gene
  AKN89_RS04990 (AKN89_04990) pilB 1062126..1063817 (-) 1692 WP_053108120.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AKN89_RS04995 (AKN89_04995) - 1064087..1064596 (+) 510 WP_053108121.1 pilin -
  AKN89_RS05000 (AKN89_05000) elbB 1064667..1065320 (-) 654 WP_053106023.1 isoprenoid biosynthesis glyoxalase ElbB -
  AKN89_RS05005 (AKN89_05005) - 1065363..1065779 (-) 417 WP_053106024.1 PACE efflux transporter -
  AKN89_RS05010 (AKN89_05010) rplQ 1065858..1066241 (-) 384 WP_053100999.1 50S ribosomal protein L17 -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44072.87 Da        Isoelectric Point: 9.7844

>NTDB_id=153828 AKN89_RS04985 WP_053108119.1 1060903..1062120(-) (pilC) [Thiopseudomonas alkaliphila strain E1148]
MEIKKSPKSYMYVWHGKSRQGNKLSGVINSHDINIVKAQLRKQGVLHAKVKKKGITFGRDKKIKSMDITVFTRQMATMMQ
AGVPLLQSFTIIIEGLDNSNMRKLLEAVRNDIAGGSSFAGALRKHSLYFDELYCNLVASGETSGALETLLDRVATYKEKS
EALKSKIKKAMNYPIAVVVVAAVVTSILLLKVVPQFQEIFEGFGAELPVFTQLVIGISELLQEYSLYVLIGLVLLLYAAR
RTLRRSEQLRNRADKILLKMPIIGDILYNSAVARYGRTLATTFSAGVPLVDALDSVSGAIGNVVFKSAVQQVKSDVSTGM
QLHTSMISSGVFPPLALQLTAIGEESGALDTMLEKVAAYYEAEVDNAVDGLTALLEPIIMVVLGVLIGGLVIAMYLPILQ
MGNAV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=153828 AKN89_RS04985 WP_053108119.1 1060903..1062120(-) (pilC) [Thiopseudomonas alkaliphila strain E1148]
ATGGAAATTAAGAAGTCGCCTAAAAGCTATATGTATGTTTGGCATGGTAAAAGCAGGCAAGGGAATAAGTTAAGTGGTGT
TATTAATTCGCATGATATTAATATTGTAAAGGCTCAGCTAAGAAAGCAGGGAGTTCTGCATGCTAAGGTGAAAAAGAAAG
GTATCACCTTTGGTCGCGATAAAAAAATTAAATCCATGGACATAACCGTCTTTACTCGGCAAATGGCCACCATGATGCAA
GCCGGGGTGCCTTTATTACAGTCGTTTACCATTATTATTGAAGGCTTAGACAATTCCAATATGCGTAAATTACTAGAAGC
TGTGCGTAATGATATTGCTGGGGGTAGCAGTTTTGCAGGAGCTTTACGCAAACACTCACTATATTTTGATGAGTTGTACT
GCAATTTAGTGGCCTCAGGCGAAACGTCGGGAGCTTTGGAAACTTTGCTTGATCGAGTAGCGACCTATAAAGAAAAGTCA
GAGGCGCTTAAATCTAAAATTAAAAAAGCCATGAACTACCCGATTGCTGTAGTGGTGGTGGCAGCTGTTGTGACGAGTAT
TTTGCTATTAAAAGTGGTACCACAGTTCCAGGAGATCTTTGAAGGTTTTGGGGCTGAATTGCCTGTATTTACGCAATTGG
TAATTGGTATTTCAGAGTTGCTGCAAGAGTATAGTTTATATGTGCTGATAGGCTTAGTACTGTTACTTTATGCTGCTCGT
AGGACGTTGCGCCGATCAGAGCAGTTGCGCAATCGTGCGGATAAAATACTGCTAAAAATGCCGATAATAGGTGATATTTT
ATATAACTCTGCTGTGGCTCGTTATGGGCGCACCTTAGCTACAACCTTCTCAGCCGGCGTACCTTTGGTTGATGCGTTAG
ATTCAGTTTCTGGCGCAATCGGCAACGTGGTTTTTAAAAGTGCAGTGCAGCAAGTGAAGTCCGATGTGTCTACTGGTATG
CAGTTGCATACTTCAATGATTTCTAGCGGGGTATTTCCTCCCCTAGCACTACAACTTACTGCTATTGGTGAAGAGTCTGG
TGCGCTAGATACCATGTTAGAAAAAGTTGCAGCGTACTATGAAGCGGAAGTAGATAACGCTGTGGATGGCTTAACTGCGT
TGCTAGAACCAATTATTATGGTGGTGCTAGGTGTACTAATTGGCGGACTAGTTATTGCCATGTACCTGCCAATCTTACAA
ATGGGTAACGCGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(277-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.012

99.506

0.647
  pilC Acinetobacter baumannii D1279779

54.412

100

0.548
  pilC Acinetobacter baylyi ADP1

53.415

100

0.541
  pilC Legionella pneumophila strain ERS1305867

49.505

99.753

0.494
  pilG Neisseria gonorrhoeae MS11

42.857

98.519

0.422
  pilG Neisseria meningitidis 44/76-A

42.607

98.519

0.42
  pilC Vibrio campbellii strain DS40M4

41.96

98.272

0.412
  pilC Vibrio cholerae strain A1552

41.071

96.79

0.398
  pilC Thermus thermophilus HB27

39.599

98.519

0.39

Multiple sequence alignment