Detailed information NTG0153827

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AKN89_RS04980
• Genome accession: NZ_CP012364
• Coordinates: 1060003..1060899 (-)
• Length: 298 a.a.
• NCBI ID: WP_053108118.1
• Uniprot ID: -
• Organism: Thiopseudomonas alkaliphila strain E1148
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1055003..1065899

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AKN89_RS04960 (AKN89_04960)	-	1056565..1057023 (+)	459	WP_053103346.1	YiiD C-terminal domain-containing protein	-
AKN89_RS04965 (AKN89_04965)	-	1057020..1058231 (+)	1212	WP_053108116.1	NAD(P)/FAD-dependent oxidoreductase	-
AKN89_RS04970 (AKN89_04970)	-	1058402..1059391 (+)	990	WP_053108117.1	hypothetical protein	-
AKN89_RS04975 (AKN89_04975)	-	1059611..1059892 (+)	282	WP_053101006.1	GIY-YIG nuclease family protein	-
AKN89_RS04980 (AKN89_04980)	pilD	1060003..1060899 (-)	897	WP_053108118.1	prepilin peptidase	Machinery gene
AKN89_RS04985 (AKN89_04985)	pilC	1060903..1062120 (-)	1218	WP_053108119.1	type II secretion system F family protein	Machinery gene
AKN89_RS04990 (AKN89_04990)	pilB	1062126..1063817 (-)	1692	WP_053108120.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AKN89_RS04995 (AKN89_04995)	-	1064087..1064596 (+)	510	WP_053108121.1	pilin	-
AKN89_RS05000 (AKN89_05000)	elbB	1064667..1065320 (-)	654	WP_053106023.1	isoprenoid biosynthesis glyoxalase ElbB	-
AKN89_RS05005 (AKN89_05005)	-	1065363..1065779 (-)	417	WP_053106024.1	PACE efflux transporter	-

Sequence

Protein

• Length: 298 a.a.
• Molecular weight: 32655.16 Da
• Isoelectric Point: 7.1636

>NTDB_id=153827 AKN89_RS04980 WP_053108118.1 1060003..1060899(-) (pilD) [Thiopseudomonas alkaliphila strain E1148]
MNDWVMVLEQPAVFITCALVLGLLVGSFLNVLVYRLPKMLQADWQQQAKEILAEAQPETAAIPELDSAKPRFNLLVPRSA
CPHCQHVIKPWENIPLLSWLALKGKCSGCQQAISKRYPFVELASGILSAVVAWKLGFGLSAMAMLLLTWGLLAMSLIDAD
HQLLPDILVLPLLWLGLLLNTQAMFVSLSDAVLGAAIGYLSLWSIYWLFKLIMGKEGMGYGDFKLLAMLGAWGGWQVLPL
TILLSSLVGAVLGIIILRLQKEGMEKPIPYGPYLAVAGWIALLWGNQITQAYLGFAGL

Nucleotide

• Length: 897 bp

>NTDB_id=153827 AKN89_RS04980 WP_053108118.1 1060003..1060899(-) (pilD) [Thiopseudomonas alkaliphila strain E1148]
ATGAATGATTGGGTAATGGTATTAGAGCAGCCTGCAGTATTTATCACCTGCGCCTTGGTGCTGGGGCTGTTGGTAGGCAG
CTTTTTAAATGTGCTGGTGTATCGTCTGCCAAAAATGCTGCAGGCCGACTGGCAACAACAAGCCAAAGAGATTCTAGCCG
AAGCCCAGCCAGAAACGGCCGCAATCCCAGAGCTTGATTCCGCGAAACCACGTTTTAACTTGTTGGTACCACGCTCAGCC
TGTCCCCATTGCCAGCATGTGATTAAGCCATGGGAGAATATTCCACTGCTTAGCTGGCTGGCGCTTAAAGGCAAGTGCTC
GGGTTGCCAGCAAGCCATTAGTAAACGCTACCCATTTGTGGAACTGGCCAGCGGCATTTTATCGGCAGTGGTGGCCTGGA
AACTTGGCTTTGGCCTATCGGCTATGGCTATGTTGCTGTTGACCTGGGGGCTACTGGCGATGAGCCTGATTGATGCCGAT
CACCAACTGCTGCCCGATATTTTAGTGCTGCCGCTGCTTTGGCTGGGCTTACTGCTCAATACCCAAGCGATGTTTGTCAG
CTTAAGTGATGCGGTATTGGGAGCTGCTATCGGTTATCTAAGCCTGTGGAGTATTTACTGGCTATTTAAGCTGATTATGG
GCAAAGAAGGCATGGGCTACGGTGATTTTAAGCTGCTGGCGATGCTGGGCGCTTGGGGCGGCTGGCAAGTCTTACCGCTA
ACAATTTTGCTGTCTTCGTTAGTAGGCGCAGTTTTGGGTATTATTATTTTGCGCTTACAAAAAGAAGGCATGGAAAAACC
CATTCCCTACGGCCCTTACCTCGCTGTCGCCGGCTGGATCGCCTTACTCTGGGGCAACCAAATCACCCAAGCTTATTTAG
GGTTTGCGGGGCTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (146-254)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.521	95.302	0.51
pilD	Vibrio campbellii strain DS40M4	50.704	95.302	0.483
pilD	Acinetobacter baumannii D1279779	50.177	94.966	0.477
pilD	Acinetobacter nosocomialis M2	50.177	94.966	0.477
pilD	Neisseria gonorrhoeae MS11	46.715	91.946	0.43