Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACZ81_RS14690 Genome accession   NZ_CP012202
Coordinates   3388009..3389217 (+) Length   402 a.a.
NCBI ID   WP_061486998.1    Uniprot ID   -
Organism   Alteromonas macleodii strain HOT1A3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3383009..3394217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACZ81_RS14665 (ACZ81_14660) ampE 3383857..3384759 (-) 903 WP_061486393.1 beta-lactamase regulator AmpE -
  ACZ81_RS14670 (ACZ81_14665) ampD 3384802..3385341 (-) 540 WP_061486394.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACZ81_RS14675 (ACZ81_14670) - 3385525..3386043 (+) 519 WP_061486395.1 retropepsin-like aspartic protease family protein -
  ACZ81_RS14680 (ACZ81_14675) nadC 3386076..3386930 (+) 855 WP_061486396.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACZ81_RS14685 (ACZ81_14680) - 3387500..3387928 (+) 429 WP_061486397.1 pilin -
  ACZ81_RS14690 (ACZ81_14685) pilC 3388009..3389217 (+) 1209 WP_061486998.1 type II secretion system F family protein Machinery gene
  ACZ81_RS14695 (ACZ81_14690) pilD 3389270..3390163 (+) 894 WP_061486398.1 prepilin peptidase Machinery gene
  ACZ81_RS14700 (ACZ81_14695) coaE 3390182..3390793 (+) 612 WP_061486399.1 dephospho-CoA kinase -
  ACZ81_RS14705 (ACZ81_14700) zapD 3391037..3391789 (+) 753 WP_039227984.1 cell division protein ZapD -
  ACZ81_RS14710 (ACZ81_14705) yacG 3391931..3392158 (+) 228 WP_061486400.1 DNA gyrase inhibitor YacG -
  ACZ81_RS14715 (ACZ81_14710) - 3392378..3393250 (+) 873 WP_061486401.1 aspartoacylase -
  ACZ81_RS14720 (ACZ81_14715) mutT 3393370..3393753 (-) 384 WP_061486402.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43669.41 Da        Isoelectric Point: 9.6631

>NTDB_id=152194 ACZ81_RS14690 WP_061486998.1 3388009..3389217(+) (pilC) [Alteromonas macleodii strain HOT1A3]
MAKAVSTFTWQGKDRHGQSKKGEITAATISEAKNLLRRQGISANKVKKLSKPLFGGAKKITPADISVISRQIATMLAAGV
TLIQSLDMIAQGHANPSMRKLLGEITDEVKSGNPLSSALRKHPLYFDDLYCDLVYTGEQSGALETIYDRIATYKEKAEAL
KSKIKKAMFYPIAVLVVAFIVTTILLIFVVPQFEEIFSSFGAELPAFTQFVLAISRFVQDYGIFIAIGIGGAGFMFMRTY
KRSQKLRDTVDRNILKIPVIGEILKKASIARFTRTLATTFAAGVPLIGALESAAGASGNAVYREAILYMRKEVAGGMPMH
VAMRATQVFPDMVTQMIAIGEESGAVDEMLSKIATIYEAEVDDMVDGLTSLLEPMIMAVLGVVIGGLIVAMYLPIFQMGN
VV

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=152194 ACZ81_RS14690 WP_061486998.1 3388009..3389217(+) (pilC) [Alteromonas macleodii strain HOT1A3]
ATGGCAAAAGCAGTATCTACATTCACATGGCAGGGTAAAGACCGTCATGGGCAATCAAAAAAAGGCGAAATAACAGCTGC
GACCATTTCAGAAGCTAAAAACCTGTTGCGACGCCAAGGCATTTCTGCAAATAAAGTTAAAAAATTATCCAAACCGCTCT
TCGGTGGTGCGAAAAAAATCACACCAGCCGACATTTCTGTCATTTCTCGTCAAATCGCCACCATGCTTGCCGCTGGGGTT
ACCCTTATTCAGTCCTTGGATATGATTGCTCAGGGACATGCAAACCCATCGATGCGGAAACTTTTAGGCGAAATTACCGA
TGAGGTTAAATCTGGTAACCCGCTCTCATCGGCCCTTAGAAAACACCCCCTATATTTTGACGATTTATATTGCGACTTGG
TTTACACCGGTGAGCAGTCGGGCGCACTTGAAACCATTTATGACCGCATCGCGACCTACAAAGAGAAAGCCGAAGCACTA
AAATCAAAAATCAAAAAAGCGATGTTTTACCCTATCGCAGTTTTGGTTGTTGCTTTTATTGTTACGACCATCCTTCTTAT
ATTTGTTGTGCCGCAGTTTGAAGAAATTTTCAGCAGCTTTGGCGCAGAACTCCCTGCCTTCACCCAGTTCGTATTGGCTA
TTTCACGGTTCGTCCAAGACTACGGTATTTTTATCGCCATAGGTATTGGTGGTGCTGGGTTTATGTTTATGCGCACCTAT
AAGCGAAGCCAGAAGCTACGTGACACGGTGGACCGCAACATTTTAAAAATTCCCGTTATCGGAGAAATTCTAAAAAAAGC
CAGCATTGCGCGCTTTACCCGAACCTTAGCCACAACCTTTGCAGCAGGTGTGCCGCTTATTGGCGCACTTGAATCCGCCG
CAGGTGCATCGGGCAACGCAGTATACCGCGAAGCCATTTTATACATGCGTAAAGAAGTGGCGGGCGGTATGCCAATGCAT
GTTGCAATGCGCGCTACACAAGTATTCCCTGACATGGTGACGCAGATGATTGCCATTGGTGAGGAGTCGGGCGCGGTTGA
TGAAATGCTTAGTAAAATTGCCACCATATACGAAGCTGAAGTCGATGATATGGTAGACGGTTTAACTAGCCTACTAGAAC
CAATGATCATGGCCGTACTTGGCGTGGTAATCGGCGGCTTGATTGTGGCTATGTACCTCCCTATATTCCAGATGGGTAAC
GTGGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.194

98.756

0.585
  pilC Acinetobacter baumannii D1279779

53.102

100

0.532
  pilC Legionella pneumophila strain ERS1305867

52.897

98.756

0.522
  pilC Acinetobacter baylyi ADP1

52.239

100

0.522
  pilG Neisseria meningitidis 44/76-A

45.7

100

0.463
  pilG Neisseria gonorrhoeae MS11

45.455

100

0.46
  pilC Vibrio cholerae strain A1552

45.363

99.254

0.45
  pilC Vibrio campbellii strain DS40M4

43.176

100

0.433
  pilC Thermus thermophilus HB27

37

99.502

0.368

Multiple sequence alignment