Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACJ67_RS10050 Genome accession   NZ_CP012020
Coordinates   2118354..2119577 (+) Length   407 a.a.
NCBI ID   WP_049638961.1    Uniprot ID   A0A0K0SWT0
Organism   Methylophilus sp. TWE2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2113354..2124577
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ67_RS10025 (ACJ67_10025) - 2113461..2114165 (-) 705 WP_049638957.1 type 1 glutamine amidotransferase -
  ACJ67_RS10030 (ACJ67_10030) - 2114162..2114713 (-) 552 WP_049638958.1 rRNA large subunit pseudouridine synthase E -
  ACJ67_RS10035 (ACJ67_10035) icd 2114724..2115962 (+) 1239 WP_369799079.1 NADP-dependent isocitrate dehydrogenase -
  ACJ67_RS10040 (ACJ67_10040) - 2116046..2116249 (-) 204 WP_013441419.1 cold-shock protein -
  ACJ67_RS10045 (ACJ67_10045) pilB 2116595..2118316 (+) 1722 WP_049638960.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJ67_RS10050 (ACJ67_10050) pilC 2118354..2119577 (+) 1224 WP_049638961.1 type II secretion system F family protein Machinery gene
  ACJ67_RS10055 (ACJ67_10055) - 2119642..2120355 (-) 714 WP_369799111.1 DsbC family protein -
  ACJ67_RS10060 (ACJ67_10060) - 2120431..2121639 (-) 1209 WP_049638963.1 FAD-dependent oxidoreductase -
  ACJ67_RS10065 (ACJ67_10065) - 2121629..2122798 (-) 1170 WP_049639868.1 FAD-dependent monooxygenase -
  ACJ67_RS10070 (ACJ67_10070) - 2122840..2124138 (-) 1299 WP_049638964.1 aminopeptidase P N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44316.23 Da        Isoelectric Point: 10.0440

>NTDB_id=150427 ACJ67_RS10050 WP_049638961.1 2118354..2119577(+) (pilC) [Methylophilus sp. TWE2]
MATGNIKQSVYQWEGKDRKGKLVKGEMRANGEAVVNATLRRQGITIIKVKKQSALVTKGKVTEKDITLFTRQLATMMKAG
VPLLQAFDIVGKGHHNPAVSKLLSDIKADVETGSSLSAAFRKYPLYFDQLFCNLVGAGEQAGILDSLLERLATYKEKILA
IKSKIKSALVYPTSIIVVAFVITAVIMIFVVPAFKDLFSSFGADLPGPTVVVINISDFFVKWWWAIFGTLGFGLWFFFYT
WKRSEKMQATMDRLMLRLPIFGELIRKATIARFARTLSTMFAAGVPLVEALDSVAGASGNKVYFDATKKIQSEVSTGTSF
TVSMQNANVFPNMVIQMTAIGEESGSLDGMLTKVADFYEGEVDDAVAAISSLMEPVIMVVLGVLIGGLVVAMYMPIFKMG
KVVSGGG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=150427 ACJ67_RS10050 WP_049638961.1 2118354..2119577(+) (pilC) [Methylophilus sp. TWE2]
ATGGCGACTGGCAATATCAAACAATCCGTGTATCAGTGGGAAGGCAAGGACCGTAAAGGCAAGCTGGTTAAAGGCGAAAT
GCGCGCCAATGGCGAAGCGGTCGTCAATGCCACTTTGCGTCGCCAGGGCATTACCATCATCAAGGTAAAGAAGCAAAGCG
CACTGGTCACCAAAGGTAAAGTCACCGAAAAAGATATCACCTTATTTACGCGTCAGCTGGCCACCATGATGAAAGCAGGC
GTGCCTTTATTGCAAGCCTTTGATATTGTTGGCAAAGGACATCACAACCCTGCAGTCTCCAAACTATTGAGCGACATTAA
AGCCGATGTCGAAACCGGTAGCAGTCTGAGTGCGGCTTTCCGTAAATACCCTTTATATTTTGATCAATTGTTCTGCAACT
TGGTGGGCGCGGGTGAACAAGCCGGTATCCTGGACAGTTTACTGGAGCGATTGGCCACCTATAAAGAAAAGATTCTTGCC
ATCAAAAGCAAAATCAAATCCGCCCTGGTTTATCCTACCTCGATTATCGTGGTAGCTTTTGTCATCACCGCCGTCATCAT
GATTTTTGTCGTGCCCGCGTTTAAAGATCTGTTCAGCAGCTTTGGGGCAGACTTGCCGGGCCCCACTGTAGTGGTCATTA
ATATATCCGACTTTTTCGTTAAATGGTGGTGGGCCATTTTCGGCACTCTGGGGTTTGGTTTATGGTTCTTTTTCTACACC
TGGAAACGCTCTGAAAAAATGCAGGCGACCATGGATCGACTCATGCTGCGCCTGCCTATTTTTGGTGAACTGATCCGTAA
AGCGACCATCGCGCGTTTTGCCCGCACACTCTCCACTATGTTTGCTGCCGGTGTCCCACTTGTTGAGGCCCTGGATTCTG
TGGCTGGTGCTTCGGGCAACAAAGTCTATTTTGATGCGACCAAGAAAATCCAAAGCGAAGTCAGCACGGGTACCAGCTTT
ACAGTGTCCATGCAGAACGCAAATGTGTTCCCCAATATGGTGATCCAGATGACGGCCATTGGGGAGGAGTCTGGCTCTTT
GGATGGCATGCTAACCAAAGTAGCAGACTTTTACGAAGGCGAAGTCGATGATGCCGTGGCCGCGATCTCTAGTTTGATGG
AGCCTGTGATTATGGTTGTCCTGGGCGTACTGATTGGCGGCCTGGTGGTTGCCATGTATATGCCTATCTTCAAAATGGGC
AAGGTGGTCAGTGGTGGAGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(69-192)

Type II secretion system (T2SS), protein F (T2SSF)

(273-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K0SWT0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.321

99.509

0.541
  pilC Legionella pneumophila strain ERS1305867

54.545

97.297

0.531
  pilC Acinetobacter baylyi ADP1

52.764

97.789

0.516
  pilC Acinetobacter baumannii D1279779

52.393

97.543

0.511
  pilG Neisseria gonorrhoeae MS11

51.493

98.771

0.509
  pilG Neisseria meningitidis 44/76-A

50.995

98.771

0.504
  pilC Vibrio cholerae strain A1552

40.441

100

0.405
  pilC Vibrio campbellii strain DS40M4

40

98.28

0.393
  pilC Thermus thermophilus HB27

37.624

99.263

0.373

Multiple sequence alignment