Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACJ67_RS10045 Genome accession   NZ_CP012020
Coordinates   2116595..2118316 (+) Length   573 a.a.
NCBI ID   WP_049638960.1    Uniprot ID   A0A0K0SX17
Organism   Methylophilus sp. TWE2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2111595..2123316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ67_RS10020 (ACJ67_10020) - 2112567..2113436 (+) 870 WP_049638956.1 lysophospholipid acyltransferase family protein -
  ACJ67_RS10025 (ACJ67_10025) - 2113461..2114165 (-) 705 WP_049638957.1 type 1 glutamine amidotransferase -
  ACJ67_RS10030 (ACJ67_10030) - 2114162..2114713 (-) 552 WP_049638958.1 rRNA large subunit pseudouridine synthase E -
  ACJ67_RS10035 (ACJ67_10035) icd 2114724..2115962 (+) 1239 WP_369799079.1 NADP-dependent isocitrate dehydrogenase -
  ACJ67_RS10040 (ACJ67_10040) - 2116046..2116249 (-) 204 WP_013441419.1 cold-shock protein -
  ACJ67_RS10045 (ACJ67_10045) pilB 2116595..2118316 (+) 1722 WP_049638960.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACJ67_RS10050 (ACJ67_10050) pilC 2118354..2119577 (+) 1224 WP_049638961.1 type II secretion system F family protein Machinery gene
  ACJ67_RS10055 (ACJ67_10055) - 2119642..2120355 (-) 714 WP_369799111.1 DsbC family protein -
  ACJ67_RS10060 (ACJ67_10060) - 2120431..2121639 (-) 1209 WP_049638963.1 FAD-dependent oxidoreductase -
  ACJ67_RS10065 (ACJ67_10065) - 2121629..2122798 (-) 1170 WP_049639868.1 FAD-dependent monooxygenase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 63043.45 Da        Isoelectric Point: 4.9921

>NTDB_id=150426 ACJ67_RS10045 WP_049638960.1 2116595..2118316(+) (pilB) [Methylophilus sp. TWE2]
MAAVESTKKLGGMARMLVQSSLMSEEDASALQEKSYAQKISFLAAALAQKKISSRDIAQSASNAFGFPFFDLDVLDQDSL
PEKTIDIKLMQSHRVMLLQQKNNVAYIALSDPTNLHSLDTIQFQMGATLFPIVVEDDKLGKWIDKIVESKDTSMSSLNSG
ADDFDLGLEESNQEQEVEIQQEVDDAPVVKFLQKVLMDAINMGASDLHFEPYEKFYRIRFRVDGVLRDMSQPPLAIKEKL
ASRIKVISNLDISEKRIPQDGRMKLILSKTRAIDFRVSTLPLIHGEKIVMRILDPTSATLGIEALGYEPEQQDALLKAIH
RPYGLVLVTGPTGSGKTVSLYTCLNLLNNPEVNISTAEDPAEIPLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMIGE
IRDLETADMAIKAASTGHMVLSTLHTNDAPTTLSRLLNMGVAPFNIASAVTLITAQRLARRLCKHCKVPLDLPKEALLNV
GFNETDLDGSWQLYGAKEGGCEHCNNGYKGRVGIYQVMPISEEMTRIIMTNGNALDIAAQAKKEGIKDLRESGLLKVKQG
LTSIEEVEAVTNE

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=150426 ACJ67_RS10045 WP_049638960.1 2116595..2118316(+) (pilB) [Methylophilus sp. TWE2]
ATGGCAGCAGTGGAAAGTACAAAGAAACTTGGTGGTATGGCACGCATGCTGGTGCAATCTTCGCTCATGAGTGAAGAAGA
CGCCTCAGCATTGCAGGAAAAATCATACGCCCAGAAAATCAGTTTCCTGGCTGCCGCACTGGCGCAAAAGAAGATATCTT
CCCGGGACATTGCGCAATCGGCATCAAATGCTTTTGGCTTTCCTTTTTTTGACCTGGACGTACTAGACCAGGACTCCCTC
CCTGAGAAAACCATCGATATCAAGCTGATGCAATCGCATCGCGTGATGCTGTTACAGCAGAAAAACAACGTCGCTTATAT
CGCCCTCTCCGATCCCACCAACCTGCACTCACTGGATACCATCCAGTTCCAGATGGGCGCTACGCTGTTCCCCATCGTGG
TCGAAGATGACAAGCTGGGTAAATGGATAGATAAAATCGTCGAATCCAAAGACACCAGCATGTCTAGCCTGAATAGCGGT
GCGGACGATTTTGACTTGGGGCTGGAAGAATCTAACCAGGAGCAAGAAGTAGAAATACAGCAGGAGGTGGACGATGCACC
TGTCGTCAAATTCCTGCAGAAGGTATTGATGGATGCCATCAATATGGGCGCATCTGACTTGCACTTTGAACCTTATGAGA
AGTTTTACCGGATCCGCTTCAGGGTAGATGGCGTATTGCGAGACATGTCGCAGCCACCCCTGGCGATTAAAGAAAAACTG
GCTTCGCGCATCAAGGTCATTTCCAATCTGGATATTTCCGAAAAAAGAATCCCGCAAGATGGCCGCATGAAGCTGATCCT
CAGCAAAACCCGCGCCATCGACTTTCGTGTCAGCACATTGCCATTAATCCATGGTGAGAAAATTGTGATGCGAATTCTGG
ACCCGACCAGCGCAACATTGGGCATTGAGGCATTAGGTTATGAGCCTGAACAGCAGGATGCTTTGCTTAAAGCAATTCAT
CGCCCTTATGGCCTGGTATTAGTGACGGGGCCGACGGGTTCCGGCAAGACAGTATCACTGTATACCTGCCTCAACCTGCT
CAATAACCCCGAAGTCAATATTTCCACTGCAGAGGATCCGGCAGAGATCCCCTTACCTGGCATCAATCAGGTGAACGTCA
ATGACAAACAGGGCCTGACATTTGCTGCAGCGCTAAAGTCTTTCCTGCGCCAGGATCCTGACATTATCATGATTGGTGAA
ATCCGTGACCTGGAAACGGCTGACATGGCCATCAAGGCGGCCTCTACCGGGCACATGGTGTTATCCACATTGCATACCAA
TGATGCGCCCACCACCTTATCGCGATTATTAAATATGGGCGTAGCCCCCTTCAACATTGCCTCGGCGGTGACGTTAATTA
CGGCACAACGCCTGGCCAGGCGCTTGTGTAAGCACTGCAAGGTACCCTTGGATCTACCGAAGGAAGCATTATTGAATGTA
GGCTTTAACGAGACGGATCTTGACGGCAGCTGGCAATTATATGGCGCCAAGGAAGGCGGCTGCGAGCATTGTAATAACGG
CTATAAGGGTCGTGTCGGGATTTACCAGGTGATGCCCATTAGCGAGGAAATGACGCGTATTATCATGACCAATGGCAATG
CGCTGGATATTGCTGCACAGGCCAAAAAAGAAGGCATCAAAGACCTGAGGGAATCAGGCCTGCTCAAAGTAAAACAAGGA
CTAACCTCCATTGAGGAAGTAGAAGCCGTGACGAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K0SX17

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.38

100

0.538
  pilF Neisseria gonorrhoeae MS11

53.792

98.953

0.532
  pilB Acinetobacter baylyi ADP1

52.522

100

0.527
  pilB Legionella pneumophila strain ERS1305867

52.083

100

0.524
  pilB Vibrio cholerae strain A1552

44.714

100

0.45
  pilB Vibrio parahaemolyticus RIMD 2210633

43.509

99.476

0.433
  pilB Vibrio campbellii strain DS40M4

43.617

98.429

0.429
  pilF Thermus thermophilus HB27

37.61

99.302

0.373

Multiple sequence alignment