Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VVCECT4999_RS03165 Genome accession   NZ_CP014636
Coordinates   625187..625630 (-) Length   147 a.a.
NCBI ID   WP_039448920.1    Uniprot ID   -
Organism   Vibrio vulnificus strain CECT 4999     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 620187..630630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VVCECT4999_RS03145 (VVCECT4999_03110) coaE 620653..621261 (-) 609 WP_039448926.1 dephospho-CoA kinase -
  VVCECT4999_RS03150 (VVCECT4999_03115) pilD 621263..622132 (-) 870 WP_038941424.1 prepilin peptidase Machinery gene
  VVCECT4999_RS03155 (VVCECT4999_03120) pilC 622225..623451 (-) 1227 WP_039448924.1 type II secretion system F family protein Machinery gene
  VVCECT4999_RS03160 (VVCECT4999_03125) pilB 623499..625187 (-) 1689 WP_039448922.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VVCECT4999_RS03165 (VVCECT4999_03130) pilA 625187..625630 (-) 444 WP_039448920.1 pilin Machinery gene
  VVCECT4999_RS03170 (VVCECT4999_03135) nadC 625853..626740 (-) 888 WP_039448918.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VVCECT4999_RS03175 (VVCECT4999_03140) ampD 626833..627405 (+) 573 WP_039448916.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VVCECT4999_RS03180 (VVCECT4999_03145) pdhR 627794..628561 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 147 a.a.        Molecular weight: 15372.56 Da        Isoelectric Point: 9.9044

>NTDB_id=148780 VVCECT4999_RS03165 WP_039448920.1 625187..625630(-) (pilA) [Vibrio vulnificus strain CECT 4999]
MKKLNKTKKQQGFTLIELMIVVAIIGVLAAVAIPAYQNYVQKTEVASASATVRGLLTNIDMYQQENGGTFPNNANLVGGT
STMNALGAITLLPVGTSGGTATFAFTEGTLKGKTASVQYSKNNTTGWSCATKNVPADSRPNSCTATY

Nucleotide


Download         Length: 444 bp        

>NTDB_id=148780 VVCECT4999_RS03165 WP_039448920.1 625187..625630(-) (pilA) [Vibrio vulnificus strain CECT 4999]
ATGAAGAAATTAAACAAAACCAAGAAACAACAAGGTTTTACCTTGATTGAGTTAATGATAGTGGTGGCGATTATTGGTGT
ATTGGCTGCTGTTGCTATTCCAGCGTATCAGAATTACGTTCAAAAAACTGAAGTTGCATCGGCATCAGCAACAGTAAGAG
GATTACTAACAAACATTGATATGTATCAGCAAGAAAATGGCGGAACTTTTCCAAACAATGCCAATTTAGTTGGCGGCACT
TCAACAATGAATGCTCTTGGTGCCATAACACTGTTGCCAGTTGGTACTAGTGGTGGAACAGCAACTTTTGCCTTTACCGA
AGGTACACTCAAAGGCAAAACAGCCTCAGTACAGTACTCAAAAAACAACACCACTGGTTGGAGCTGTGCGACAAAAAATG
TACCTGCAGATTCTCGTCCAAATAGTTGTACTGCCACTTATTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)

Pilin (bacterial filament) (Pilin)

(42-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

48.462

88.435

0.429
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.735

100

0.408
  pilA Vibrio cholerae strain A1552

39.735

100

0.408
  pilA Vibrio cholerae C6706

39.735

100

0.408
  pilA Pseudomonas aeruginosa PAK

39.597

100

0.401
  pilA Ralstonia pseudosolanacearum GMI1000

38.889

97.959

0.381
  pilA2 Legionella pneumophila str. Paris

36.242

100

0.367
  pilA/pilA1 Eikenella corrodens VA1

35.294

100

0.367