Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VVCECT4999_RS03160 Genome accession   NZ_CP014636
Coordinates   623499..625187 (-) Length   562 a.a.
NCBI ID   WP_039448922.1    Uniprot ID   -
Organism   Vibrio vulnificus strain CECT 4999     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 618499..630187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VVCECT4999_RS03130 (VVCECT4999_03095) rplS 619046..619399 (+) 354 WP_011079544.1 50S ribosomal protein L19 -
  VVCECT4999_RS03135 (VVCECT4999_03100) yacG 619623..619817 (-) 195 WP_038941426.1 DNA gyrase inhibitor YacG -
  VVCECT4999_RS03140 (VVCECT4999_03105) zapD 619880..620620 (-) 741 WP_017421189.1 cell division protein ZapD -
  VVCECT4999_RS03145 (VVCECT4999_03110) coaE 620653..621261 (-) 609 WP_039448926.1 dephospho-CoA kinase -
  VVCECT4999_RS03150 (VVCECT4999_03115) pilD 621263..622132 (-) 870 WP_038941424.1 prepilin peptidase Machinery gene
  VVCECT4999_RS03155 (VVCECT4999_03120) pilC 622225..623451 (-) 1227 WP_039448924.1 type II secretion system F family protein Machinery gene
  VVCECT4999_RS03160 (VVCECT4999_03125) pilB 623499..625187 (-) 1689 WP_039448922.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VVCECT4999_RS03165 (VVCECT4999_03130) pilA 625187..625630 (-) 444 WP_039448920.1 pilin Machinery gene
  VVCECT4999_RS03170 (VVCECT4999_03135) nadC 625853..626740 (-) 888 WP_039448918.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VVCECT4999_RS03175 (VVCECT4999_03140) ampD 626833..627405 (+) 573 WP_039448916.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VVCECT4999_RS03180 (VVCECT4999_03145) pdhR 627794..628561 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62464.50 Da        Isoelectric Point: 5.5349

>NTDB_id=148779 VVCECT4999_RS03160 WP_039448922.1 623499..625187(-) (pilB) [Vibrio vulnificus strain CECT 4999]
MMSNLSTILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELA
NLVEVSDDEIESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=148779 VVCECT4999_RS03160 WP_039448922.1 623499..625187(-) (pilB) [Vibrio vulnificus strain CECT 4999]
ATGATGTCGAATCTCTCCACTATTCTGCGTCAGGCCAACCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAAACCAT
CCAAGCTTCCGGTGTCACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCGGATGAATTAACCGAGCAGCTAA
GCGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTGCGTGAG
CTGATCACCCGTCACCAAGCACTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCAGACCCGACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGCGTGCAGGTGGAGATTGTTTTAGCCGATTGCCAAGAACTGCAAGCGG
CGATTCGTAGACTTTATGGAAAGAGCATAAAACAGAGCCAAAGCTCTGGGCTAAAAGAGATTCACCAAGATGAGCTGGCG
AATTTGGTCGAGGTTTCAGATGACGAAATCGAGTCCATTGAAGACTTAAGCCAAGATGAATCCCCCGTTAGCCGTTTTAT
CAACCAAATTCTGCTTGATGCGGTGCGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTAC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCTCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAA
ATCCTCTCCAAGCTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGCATTAAGCTACGCCTTAACCAAGAAACCGC
GATTGATATGCGCGTTTCCACTTTACCTACGCTGTTTGGTGAAAAAATTGTGCTGCGACTTCTCGACAGCTCGAGTAGCA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAACAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACGGGCCCAACAGGAAGCGGCAAAACCGTATCTCTGTACACAGGCTTGAGTTTACTTAACCGGCCAGAAGT
GAATATCTCCACCGCGGAAGACCCGGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCCGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGTCACCTTGTGCTATCCACCTTACATACCAACTCGGCAGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACCTGGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCGCATCCATACAACGCAACATTTGAGTTACTCGGTCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAGCAGCTTGCAGAGGCGATCGTGAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTTGCTCGGCAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.868

100

0.769
  pilB Vibrio parahaemolyticus RIMD 2210633

76.335

100

0.763
  pilB Vibrio cholerae strain A1552

74.423

100

0.746
  pilB Legionella pneumophila strain ERS1305867

52.827

91.281

0.482
  pilB Acinetobacter baumannii D1279779

51.533

92.883

0.479
  pilB Acinetobacter baylyi ADP1

50.383

92.883

0.468
  pilF Neisseria gonorrhoeae MS11

45.31

100

0.456
  pilF Thermus thermophilus HB27

40.608

99.466

0.404
  pilB/pilB1 Synechocystis sp. PCC 6803

36.888

100

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.306

0.363