Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   WV34_RS12590 Genome accession   NZ_CP011252
Coordinates   2496312..2496926 (-) Length   204 a.a.
NCBI ID   WP_088613149.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain MT45     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2491312..2501926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WV34_RS12565 (WV34_12265) rpsT 2491612..2491878 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  WV34_RS12570 (WV34_12270) holA 2491896..2492937 (-) 1042 Protein_2423 DNA polymerase III subunit delta -
  WV34_RS19950 - 2492977..2493131 (-) 155 Protein_2424 hypothetical protein -
  WV34_RS12575 (WV34_12275) - 2493172..2493306 (+) 135 WP_003152870.1 YqzM family protein -
  WV34_RS12580 (WV34_12280) comEC 2493325..2495676 (-) 2352 WP_088613147.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  WV34_RS12585 (WV34_12285) - 2495677..2496246 (-) 570 WP_088613148.1 ComE operon protein 2 -
  WV34_RS12590 (WV34_12290) comEA 2496312..2496926 (-) 615 WP_088613149.1 helix-hairpin-helix domain-containing protein Machinery gene
  WV34_RS12595 (WV34_12295) comER 2496986..2497807 (+) 822 WP_088613150.1 late competence protein ComER -
  WV34_RS12600 (WV34_12300) - 2497876..2498613 (-) 738 WP_088613151.1 class I SAM-dependent methyltransferase -
  WV34_RS12605 (WV34_12305) rsfS 2498610..2498966 (-) 357 WP_045510454.1 ribosome silencing factor -
  WV34_RS12610 (WV34_12310) yqeK 2498983..2499543 (-) 561 WP_088613152.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  WV34_RS12615 (WV34_12315) - 2499533..2500102 (-) 570 WP_045510448.1 nicotinate-nucleotide adenylyltransferase -
  WV34_RS12620 (WV34_12320) yhbY 2500113..2500403 (-) 291 WP_014470707.1 ribosome assembly RNA-binding protein YhbY -
  WV34_RS12625 (WV34_12325) aroE 2500397..2501239 (-) 843 WP_014470708.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22271.52 Da        Isoelectric Point: 6.0432

>NTDB_id=143900 WV34_RS12590 WP_088613149.1 2496312..2496926(-) (comEA) [Bacillus amyloliquefaciens strain MT45]
MTKVMMTERQRQYLKILGVCLAAGAVFICILLFRPEKEEPVKQQSVLGQTASAPVTDKKDTADDKIMIDIKGAVRHPGVY
EMKAGDRVTQAIKKAGGLKEKADELNVNLAEQLQDGTVVYIPSEGEKENPTKTAAGEKENASVNINTASLDELQAIPGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=143900 WV34_RS12590 WP_088613149.1 2496312..2496926(-) (comEA) [Bacillus amyloliquefaciens strain MT45]
ATGACGAAAGTGATGATGACGGAGCGGCAAAGGCAGTATCTGAAAATTCTAGGCGTTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTTGCTGTTTCGTCCCGAAAAAGAAGAGCCTGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCTTCAGCAC
CCGTCACGGACAAAAAAGACACCGCAGACGATAAAATTATGATTGATATTAAAGGAGCGGTCCGACATCCCGGCGTGTAT
GAAATGAAGGCGGGGGACAGAGTGACGCAAGCCATTAAGAAAGCCGGCGGTCTGAAGGAAAAGGCGGATGAACTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGAACGGTCGTTTATATCCCGAGTGAAGGCGAAAAGGAGAATCCCACGAAAACCG
CAGCAGGTGAAAAGGAGAATGCTTCTGTCAATATCAATACGGCATCTCTTGACGAACTTCAGGCGATTCCCGGTGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGGCGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGTTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

57.949

95.588

0.554
  comEA Lactococcus lactis subsp. cremoris KW2

35.455

100

0.382
  comEA Staphylococcus aureus MW2

35.294

100

0.382
  comEA/celA/cilE Streptococcus pneumoniae D39

35.484

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae R6

35.484

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.484

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae Rx1

35.484

100

0.377
  comEA/celA/cilE Streptococcus mitis SK321

35.023

100

0.373
  comEA/celA/cilE Streptococcus mitis NCTC 12261

34.562

100

0.368
  comEA Latilactobacillus sakei subsp. sakei 23K

32.051

100

0.368

Multiple sequence alignment