Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   WR47_RS21415 Genome accession   NZ_CP011151
Coordinates   4317557..4318600 (-) Length   347 a.a.
NCBI ID   WP_001013177.1    Uniprot ID   -
Organism   Bacillus cereus strain CMCC P0021     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4312557..4323600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WR47_RS21370 (WR47_21920) - 4312991..4313191 (-) 201 WP_000106083.1 YqzE family protein -
  WR47_RS21375 (WR47_21925) aroK 4313230..4313727 (-) 498 WP_000836649.1 shikimate kinase AroK -
  WR47_RS21380 (WR47_21930) - 4313843..4314493 (-) 651 WP_000183853.1 2OG-Fe(II) oxygenase -
  WR47_RS21385 (WR47_21935) comGG 4314667..4315038 (-) 372 WP_001214374.1 competence type IV pilus minor pilin ComGG -
  WR47_RS21390 (WR47_21940) comGF 4315035..4315505 (-) 471 WP_000923097.1 competence type IV pilus minor pilin ComGF -
  WR47_RS21395 (WR47_21945) - 4315475..4315777 (-) 303 WP_000829462.1 hypothetical protein -
  WR47_RS21400 (WR47_21950) comGD 4315770..4316225 (-) 456 WP_002094140.1 competence type IV pilus minor pilin ComGD -
  WR47_RS21405 (WR47_21955) comGC 4316222..4316521 (-) 300 WP_001243321.1 competence type IV pilus major pilin ComGC -
  WR47_RS21410 (WR47_21960) comGB 4316533..4317564 (-) 1032 WP_000475180.1 competence type IV pilus assembly protein ComGB -
  WR47_RS21415 (WR47_21965) comGA 4317557..4318600 (-) 1044 WP_001013177.1 competence type IV pilus ATPase ComGA Machinery gene
  WR47_RS21420 (WR47_21970) - 4318806..4319501 (+) 696 WP_000434124.1 helix-turn-helix transcriptional regulator -
  WR47_RS21425 (WR47_21975) - 4319628..4319870 (+) 243 WP_000375619.1 DUF2626 domain-containing protein -
  WR47_RS21430 (WR47_21980) - 4319978..4321372 (+) 1395 WP_001094344.1 L-cystine transporter -
  WR47_RS33010 (WR47_21985) - 4321452..4321844 (-) 393 Protein_4276 hypothetical protein -
  WR47_RS21440 (WR47_21990) - 4321932..4322147 (-) 216 WP_001008326.1 DUF3912 family protein -
  WR47_RS21445 (WR47_21995) - 4322401..4322712 (+) 312 WP_001093240.1 hypothetical protein -
  WR47_RS21450 (WR47_22000) - 4322747..4323235 (-) 489 WP_000764263.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39177.60 Da        Isoelectric Point: 8.8944

>NTDB_id=142898 WR47_RS21415 WP_001013177.1 4317557..4318600(-) (comGA) [Bacillus cereus strain CMCC P0021]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRHCIKKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEDDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=142898 WR47_RS21415 WP_001013177.1 4317557..4318600(-) (comGA) [Bacillus cereus strain CMCC P0021]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACATTGTATTAAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTGCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTCATGACAACGTTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATAAGAAAAGGATATGCTTTAGGTTTTTTAGAAGATGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.196

100

0.562
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369

Multiple sequence alignment