Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VU14_RS03365 Genome accession   NZ_CP011100
Coordinates   730164..731273 (-) Length   369 a.a.
NCBI ID   WP_045789003.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain AH10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 725164..736273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VU14_RS03345 (VU14_03345) brnQ 725621..726928 (-) 1308 WP_080938320.1 branched-chain amino acid transport system II carrier protein -
  VU14_RS03350 (VU14_03350) - 727120..727830 (-) 711 WP_045789000.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  VU14_RS03355 (VU14_03355) srmB 727959..729182 (+) 1224 WP_045789001.1 ATP-dependent RNA helicase SrmB -
  VU14_RS03360 (VU14_03360) yaaA 729359..730132 (-) 774 WP_045789002.1 peroxide stress protein YaaA -
  VU14_RS03365 (VU14_03365) pilU 730164..731273 (-) 1110 WP_045789003.1 type IVa pilus ATPase TapU Machinery gene
  VU14_RS03370 (VU14_03370) pilT 731302..732336 (-) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  VU14_RS03375 (VU14_03375) - 732376..733077 (+) 702 WP_045789004.1 YggS family pyridoxal phosphate-dependent enzyme -
  VU14_RS03380 (VU14_03380) proC 733136..733960 (+) 825 WP_045789005.1 pyrroline-5-carboxylate reductase -
  VU14_RS03385 (VU14_03385) - 734001..734552 (+) 552 WP_016351969.1 YggT family protein -
  VU14_RS03390 (VU14_03390) yggU 734552..734851 (+) 300 WP_011707385.1 DUF167 family protein YggU -
  VU14_RS03395 (VU14_03395) - 734870..735289 (+) 420 WP_011707384.1 DUF4426 domain-containing protein -
  VU14_RS03400 (VU14_03400) - 735338..735649 (-) 312 WP_045789006.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41021.14 Da        Isoelectric Point: 6.5034

>NTDB_id=142130 VU14_RS03365 WP_045789003.1 730164..731273(-) (pilU) [Aeromonas hydrophila strain AH10]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFDRYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=142130 VU14_RS03365 WP_045789003.1 730164..731273(-) (pilU) [Aeromonas hydrophila strain AH10]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCATCTGCAGTCGCTGGCAGAAGCCCCCCTCGACAAGGCAAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGATCGCTACCTGCACACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAACTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATAGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCACCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCCCCTGACGTGATCCTGATCGGTGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAATCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCACAGCA
ACTGCTGCCCAGCCTGGACGGACGCCGCCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCG
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36

Multiple sequence alignment