Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AU380_RS01340 Genome accession   NZ_CP013696
Coordinates   293478..293942 (-) Length   154 a.a.
NCBI ID   WP_058150672.1    Uniprot ID   P18774
Organism   Pseudomonas aeruginosa strain 12-4-4(59)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 288478..298942
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AU380_RS01325 (AU380_01325) nadC 290988..291836 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AU380_RS01335 (AU380_01335) - 292017..293402 (-) 1386 WP_058150674.1 O-antigen ligase family protein -
  AU380_RS01340 (AU380_01340) pilA 293478..293942 (-) 465 WP_058150672.1 pilin Machinery gene
  AU380_RS01345 (AU380_01345) pilB 294173..295873 (+) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AU380_RS01350 (AU380_01350) pilC 295877..297094 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  AU380_RS01355 (AU380_01355) pilD 297095..297967 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  AU380_RS01360 (AU380_01360) coaE 297964..298575 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  AU380_RS01365 (AU380_01365) yacG 298572..298772 (+) 201 WP_058150655.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16277.63 Da        Isoelectric Point: 8.4578

>NTDB_id=142010 AU380_RS01340 WP_058150672.1 293478..293942(-) (pilA) [Pseudomonas aeruginosa strain 12-4-4(59)]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSATPKDTQYDIGFTE
STLLDGSGKSQIQVTDNQDGTVELVATLGKSSGSAIKGAVITVSRKNDGVWNCKITKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=142010 AU380_RS01340 WP_058150672.1 293478..293942(-) (pilA) [Pseudomonas aeruginosa strain 12-4-4(59)]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTTGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCAGCGCGACTCCTAAAGATACCCAGTATGACATTGGCTTCACCGAG
TCTACTTTGCTAGATGGTTCTGGTAAGAGTCAGATCCAGGTAACGGACAATCAAGATGGCACCGTTGAGTTGGTCGCTAC
CTTGGGTAAATCTTCTGGTTCCGCCATCAAAGGGGCTGTAATCACTGTTTCGCGTAAAAATGACGGAGTCTGGAACTGCA
AAATCACCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 6BBK

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

44.737

98.701

0.442
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.444

99.351

0.442
  pilA Pseudomonas aeruginosa PAK

45.985

88.961

0.409
  pilA2 Legionella pneumophila strain ERS1305867

39.073

98.052

0.383
  pilA Vibrio parahaemolyticus RIMD 2210633

37.255

99.351

0.37
  pilA2 Legionella pneumophila str. Paris

38

97.403

0.37
  pilA Vibrio cholerae strain A1552

36.774

100

0.37
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.774

100

0.37
  pilA Vibrio cholerae C6706

36.774

100

0.37