Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   KO116_RS11780 Genome accession   NZ_CP011052
Coordinates   2536272..2537501 (+) Length   409 a.a.
NCBI ID   WP_035558534.1    Uniprot ID   A0A0D5M435
Organism   Halomonas sp. KO116     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2531272..2542501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KO116_RS25790 (KO116_02367) - 2531590..2531802 (+) 213 WP_235272371.1 hypothetical protein -
  KO116_RS11760 (KO116_02368) - 2532182..2532997 (-) 816 WP_035558522.1 inositol monophosphatase family protein -
  KO116_RS11765 (KO116_02369) - 2533092..2533541 (-) 450 WP_035558525.1 anti-virulence regulator CigR family protein -
  KO116_RS26195 (KO116_02370) - 2533786..2534193 (-) 408 WP_035558528.1 pilin -
  KO116_RS11775 (KO116_02371) pilB 2534496..2536268 (+) 1773 WP_035558531.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KO116_RS11780 (KO116_02372) pilC 2536272..2537501 (+) 1230 WP_035558534.1 type II secretion system F family protein Machinery gene
  KO116_RS11785 (KO116_02373) pilD 2537564..2538409 (+) 846 WP_035558537.1 A24 family peptidase Machinery gene
  KO116_RS11790 (KO116_02374) coaE 2538519..2539121 (+) 603 WP_035558541.1 dephospho-CoA kinase -
  KO116_RS11795 (KO116_02375) yacG 2539155..2539388 (+) 234 WP_035558545.1 DNA gyrase inhibitor YacG -
  KO116_RS11800 (KO116_02376) - 2539440..2540315 (+) 876 WP_144408585.1 DNA replication terminus site-binding protein -
  KO116_RS11805 (KO116_02377) - 2540346..2540687 (-) 342 WP_035558550.1 hypothetical protein -
  KO116_RS25800 - 2541131..2541373 (+) 243 WP_235272561.1 helix-turn-helix transcriptional regulator -
  KO116_RS25805 (KO116_02378) - 2541445..2541744 (+) 300 WP_235272373.1 hypothetical protein -
  KO116_RS11815 (KO116_02379) - 2541975..2542349 (-) 375 WP_008957328.1 histone-like nucleoid-structuring protein, MvaT/MvaU family -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44791.55 Da        Isoelectric Point: 10.3691

>NTDB_id=141834 KO116_RS11780 WP_035558534.1 2536272..2537501(+) (pilC) [Halomonas sp. KO116]
MANARRKRRPSLQLYRWKWAGKGPQDRALSGETLGRNKAEVTAELAGQSINVKRIRKINSLSGAGRIKPTDVMVFARQMA
TMIRAGIPLLQAFQVVGESLKKPAMAMLVEQMRSDVSSGSSFSDALRRHPKHFDQLFVNLIDAGEQAGALDQMLDRVATY
KEKVESLKARVKKALWYPSAVMIVGISVTLLLLIKVVPQFESMFSNFDAELPALTQMTVNLSELAQRYWLVALGSLVATG
LAIKISVQRSPKIAYGMDVLVLRLPVIGDIMHKSALARFARTLATTFASGVPLMEGLETASGAVGNKVYERAVIQTRKDV
ATGQQLHFAMRMTNRFPPLAIQMVSIGEESGSLDAMLNRVADYYEEEVDNKVDALTSLMEPIIIVVLGVLVGGVVVSIYL
PIFSLGSAL

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=141834 KO116_RS11780 WP_035558534.1 2536272..2537501(+) (pilC) [Halomonas sp. KO116]
ATGGCAAACGCTCGGCGTAAGCGTCGCCCATCTCTTCAACTTTATCGTTGGAAATGGGCGGGCAAAGGCCCTCAAGACAG
AGCACTGAGCGGCGAAACCCTTGGCCGTAATAAAGCGGAGGTAACGGCTGAACTGGCGGGGCAAAGCATCAATGTTAAGC
GCATTCGCAAAATAAACAGCCTCAGCGGCGCAGGCCGTATTAAGCCTACCGACGTGATGGTGTTTGCGCGCCAAATGGCG
ACCATGATTCGCGCCGGTATTCCTTTGTTACAGGCTTTCCAAGTCGTCGGCGAAAGTCTGAAAAAGCCTGCCATGGCGAT
GTTGGTAGAGCAGATGAGGAGCGATGTTTCATCGGGGTCAAGCTTTTCTGATGCGTTACGCCGTCACCCAAAACATTTTG
ATCAATTGTTTGTTAACCTCATCGATGCTGGCGAACAAGCCGGTGCGCTGGATCAGATGCTGGATCGTGTCGCGACTTAT
AAAGAAAAAGTGGAATCACTAAAAGCGCGCGTTAAGAAAGCCCTTTGGTACCCTAGCGCCGTGATGATCGTCGGGATATC
GGTCACCCTGCTGCTGCTGATTAAGGTCGTCCCCCAGTTTGAAAGCATGTTTAGTAATTTTGATGCGGAGCTTCCTGCCC
TGACTCAAATGACCGTTAACTTATCCGAACTTGCTCAGCGCTATTGGCTTGTGGCCCTGGGTAGCCTGGTGGCCACGGGG
CTAGCCATCAAAATAAGCGTTCAACGCTCACCCAAAATTGCTTATGGCATGGACGTTTTGGTGCTTCGCTTACCGGTGAT
CGGCGACATTATGCATAAATCCGCCCTTGCCCGTTTTGCGCGCACCTTAGCAACGACCTTTGCTTCCGGCGTACCGTTGA
TGGAAGGTTTAGAGACCGCCTCTGGGGCCGTGGGCAATAAAGTTTATGAACGCGCCGTCATACAAACCCGTAAAGATGTC
GCGACCGGGCAGCAGCTACACTTTGCCATGCGCATGACCAACCGCTTTCCTCCCCTGGCAATTCAAATGGTCAGCATCGG
CGAAGAGTCGGGGTCGTTGGATGCCATGTTGAACCGCGTCGCTGATTATTACGAAGAAGAAGTCGACAATAAAGTCGACG
CCCTCACCTCCCTAATGGAGCCGATTATTATCGTCGTATTGGGCGTACTGGTGGGCGGCGTCGTTGTGTCTATATACTTG
CCAATCTTCAGCTTAGGCTCGGCTCTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D5M435

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

51.654

96.088

0.496
  pilC Acinetobacter baumannii D1279779

51.948

94.132

0.489
  pilC Acinetobacter baylyi ADP1

50.773

94.866

0.482
  pilC Legionella pneumophila strain ERS1305867

47.537

99.267

0.472
  pilC Vibrio campbellii strain DS40M4

43.596

99.267

0.433
  pilC Vibrio cholerae strain A1552

41.872

99.267

0.416
  pilG Neisseria meningitidis 44/76-A

42.317

97.066

0.411
  pilG Neisseria gonorrhoeae MS11

42.317

97.066

0.411

Multiple sequence alignment