Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KO116_RS11775 Genome accession   NZ_CP011052
Coordinates   2534496..2536268 (+) Length   590 a.a.
NCBI ID   WP_035558531.1    Uniprot ID   A0A0D5M3W0
Organism   Halomonas sp. KO116     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2529496..2541268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KO116_RS11740 (KO116_02364) - 2530098..2530748 (+) 651 WP_045812123.1 WYL domain-containing protein -
  KO116_RS11745 (KO116_02365) - 2530790..2531305 (+) 516 WP_052703760.1 gamma-glutamylcyclotransferase family protein -
  KO116_RS25790 (KO116_02367) - 2531590..2531802 (+) 213 WP_235272371.1 hypothetical protein -
  KO116_RS11760 (KO116_02368) - 2532182..2532997 (-) 816 WP_035558522.1 inositol monophosphatase family protein -
  KO116_RS11765 (KO116_02369) - 2533092..2533541 (-) 450 WP_035558525.1 anti-virulence regulator CigR family protein -
  KO116_RS26195 (KO116_02370) - 2533786..2534193 (-) 408 WP_035558528.1 pilin -
  KO116_RS11775 (KO116_02371) pilB 2534496..2536268 (+) 1773 WP_035558531.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KO116_RS11780 (KO116_02372) pilC 2536272..2537501 (+) 1230 WP_035558534.1 type II secretion system F family protein Machinery gene
  KO116_RS11785 (KO116_02373) pilD 2537564..2538409 (+) 846 WP_035558537.1 A24 family peptidase Machinery gene
  KO116_RS11790 (KO116_02374) coaE 2538519..2539121 (+) 603 WP_035558541.1 dephospho-CoA kinase -
  KO116_RS11795 (KO116_02375) yacG 2539155..2539388 (+) 234 WP_035558545.1 DNA gyrase inhibitor YacG -
  KO116_RS11800 (KO116_02376) - 2539440..2540315 (+) 876 WP_144408585.1 DNA replication terminus site-binding protein -
  KO116_RS11805 (KO116_02377) - 2540346..2540687 (-) 342 WP_035558550.1 hypothetical protein -

Sequence

Protein


Download         Length: 590 a.a.        Molecular weight: 64469.87 Da        Isoelectric Point: 6.2069

>NTDB_id=141833 KO116_RS11775 WP_035558531.1 2534496..2536268(+) (pilB) [Halomonas sp. KO116]
MSQATTESTLSYESTLSHAAARGGQRGIAQRLIKHGLLTEAQAAAAASAAVENDISLLEHVVESGLVPPTAAAIAAGYEY
GLAVINLETVRISSLPPVADYPVRILQSLNVVPLARHGHRLTVAVPYPATLTQLDELQFATGLSIEGILAPMDQLATTLS
SYLALTQSERGMLDEFNDLDDELSTLSVAEDSEVPLEEAPQNSDDGPVVKFVNKILLDAIRRGASDIHFEPYETQYRIRF
RIDGILIEVARPPLGMRNRVAARLKIMARLDISERRLPQDGAIKLKLSRTRSLDFRVNSLPTVYGEKLVLRILDPSAAQL
GIEQLGFTDDQRHFYERALNQPQGMILVSGPTGSGKTVSLYTGINILNKVERNICTAEDPVEIKVAGINQVNVLPKIGLD
FASALRAFLRQDPDVVMVGEIRDLETADIAVKASQTGHLVLSTVHTNSAAETLTRLANMGVTSFNIASAVSLIIAQRLTR
KLCPRCKEPVVIPHEALRKEGFSDEEIQQATIYKPVGCKHCTHGYKGRVGIYEVVPITEAMRQLIMSNANAMDIDQQARR
EGYPSLHQSGLLKVMRGITSLEEINRVSKE

Nucleotide


Download         Length: 1773 bp        

>NTDB_id=141833 KO116_RS11775 WP_035558531.1 2534496..2536268(+) (pilB) [Halomonas sp. KO116]
ATGAGCCAAGCGACTACCGAATCAACCTTAAGTTATGAGTCAACCCTAAGCCACGCCGCCGCACGCGGCGGGCAGCGCGG
CATTGCCCAGCGTTTAATCAAGCACGGCTTGCTAACCGAAGCGCAGGCGGCGGCGGCCGCTAGCGCCGCCGTGGAAAACG
ATATTTCACTGTTAGAACACGTGGTTGAAAGCGGGCTTGTGCCCCCCACCGCTGCGGCGATTGCCGCAGGGTACGAGTAC
GGGCTAGCGGTTATCAATTTAGAAACCGTTCGTATTAGCTCGCTGCCCCCTGTTGCTGACTACCCAGTGAGGATTCTGCA
AAGCTTAAATGTGGTGCCTTTGGCCCGTCATGGGCACCGTTTAACGGTGGCCGTGCCTTACCCCGCCACGCTTACCCAGC
TTGATGAGTTACAGTTTGCCACTGGGCTTAGCATTGAAGGGATATTAGCCCCGATGGACCAGCTAGCCACCACGCTGAGT
AGTTATTTAGCATTAACGCAAAGCGAACGTGGCATGCTGGACGAGTTTAATGACCTCGACGATGAGTTATCGACGCTGAG
CGTTGCCGAAGACAGCGAAGTGCCTTTGGAAGAGGCCCCCCAAAACAGTGATGATGGCCCCGTCGTTAAGTTTGTTAATA
AGATTTTATTAGATGCGATTCGTCGTGGTGCCTCCGACATTCATTTCGAGCCTTACGAAACGCAGTATCGTATCCGTTTT
CGTATTGACGGCATCTTAATTGAGGTCGCACGCCCTCCCTTGGGGATGCGTAACCGTGTTGCCGCCCGCTTGAAAATTAT
GGCGCGGCTGGATATTTCTGAGCGGCGCCTGCCCCAAGACGGAGCGATAAAACTTAAATTATCGCGCACGCGCTCGCTCG
ATTTTCGGGTCAACTCACTGCCCACGGTGTATGGCGAAAAATTGGTATTGCGTATACTCGACCCCTCCGCGGCCCAGCTC
GGCATCGAGCAATTAGGCTTTACTGATGATCAACGTCACTTTTATGAACGTGCCCTCAATCAGCCCCAGGGAATGATACT
GGTGTCGGGCCCCACTGGCAGTGGCAAAACCGTGTCGCTGTATACCGGCATTAATATTCTCAATAAAGTGGAGCGCAATA
TTTGCACGGCGGAAGATCCGGTTGAGATTAAAGTAGCCGGTATTAATCAGGTCAACGTGTTACCTAAAATTGGGCTTGAT
TTTGCCAGTGCGCTACGGGCATTTCTGCGCCAAGACCCGGATGTGGTCATGGTGGGTGAAATTCGGGATTTAGAAACCGC
TGACATTGCCGTTAAAGCCTCCCAGACAGGCCACTTGGTGCTTTCTACCGTGCACACTAACTCAGCCGCCGAAACGCTCA
CGCGGCTGGCGAACATGGGGGTAACGTCGTTTAACATCGCCAGCGCCGTTAGCCTGATTATTGCCCAACGCTTAACGCGT
AAACTCTGCCCACGCTGCAAGGAGCCGGTTGTTATCCCCCACGAGGCACTGCGCAAAGAGGGCTTTAGCGATGAAGAAAT
TCAACAGGCCACTATTTACAAGCCGGTAGGTTGTAAGCACTGCACGCATGGCTATAAAGGTCGCGTGGGTATTTATGAGG
TCGTTCCCATTACGGAAGCCATGCGCCAATTAATTATGAGCAACGCCAACGCGATGGATATCGATCAGCAGGCGCGCCGC
GAAGGCTACCCCAGTTTGCACCAGAGCGGTTTATTAAAAGTCATGCGGGGGATTACCAGCCTTGAAGAAATTAACCGCGT
CAGTAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-164)

Type II/IV secretion system protein (T2SSE)

(214-481)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D5M3W0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.15

95.763

0.49
  pilB Acinetobacter baumannii D1279779

49.735

96.102

0.478
  pilB Vibrio campbellii strain DS40M4

50.54

94.237

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

49.734

95.424

0.475
  pilB Acinetobacter baylyi ADP1

49.117

95.932

0.471
  pilB Vibrio cholerae strain A1552

54.167

81.356

0.441
  pilF Neisseria gonorrhoeae MS11

50.625

81.356

0.412

Multiple sequence alignment