Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSPO_RS10615 Genome accession   NZ_CP011039
Coordinates   2288534..2290216 (+) Length   560 a.a.
NCBI ID   WP_010561749.1    Uniprot ID   -
Organism   Pseudoalteromonas spongiae UST010723-006     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2283534..2295216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSPO_RS10595 (PSPO_a2381) pilB 2283901..2285586 (+) 1686 WP_010561745.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSPO_RS10600 (PSPO_a2382) pilC 2285657..2286886 (+) 1230 WP_010561746.1 type II secretion system F family protein Machinery gene
  PSPO_RS10605 (PSPO_a2383) pilD 2286893..2287777 (+) 885 WP_010561747.1 prepilin peptidase Machinery gene
  PSPO_RS10610 (PSPO_a2384) coaE 2287779..2288372 (+) 594 WP_010561748.1 dephospho-CoA kinase -
  PSPO_RS10615 (PSPO_a2387) pilB 2288534..2290216 (+) 1683 WP_010561749.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSPO_RS10620 (PSPO_a2388) yacG 2290384..2290617 (+) 234 WP_010561750.1 DNA gyrase inhibitor YacG -
  PSPO_RS10625 (PSPO_a2389) - 2290634..2291356 (-) 723 WP_010561751.1 hypothetical protein -
  PSPO_RS10630 (PSPO_a2390) - 2291549..2292004 (-) 456 WP_010561752.1 Spy/CpxP family protein refolding chaperone -
  PSPO_RS10635 (PSPO_a2391) - 2292159..2292839 (+) 681 WP_010561753.1 response regulator transcription factor -
  PSPO_RS10640 (PSPO_a2392) - 2292843..2294222 (+) 1380 WP_010561754.1 ATP-binding protein -
  PSPO_RS10645 (PSPO_a2393) - 2294215..2295135 (+) 921 WP_010561755.1 alpha/beta fold hydrolase -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 61350.23 Da        Isoelectric Point: 4.9572

>NTDB_id=141690 PSPO_RS10615 WP_010561749.1 2288534..2290216(+) (pilB) [Pseudoalteromonas spongiae UST010723-006]
MNVNSPLIRKCVAMGFIAHEQVISAASSTTHISEFILNNSKLSSKQLAGYCSSWFNLPVLDIATVEPSSIPLDDISEELV
KKHHLLPLSKCGRTLFLATSDPTNFDAFEDFEFNTGLQTEAIVVDFNALEQLIETIFNKTDDLAISDEEFEQFGDIEVTD
TEANLSEDSAPDKDDAPIIVYINKILMDAIRKGASDLHFEPYEKSYRIRFRIDGILHEVASPPQMLSSRISARVKVMSRL
DIAEKRKPQDGRIKLKITAKKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEPAQKAMYLDALSQPQGMILVTG
PTGSGKTVSLYTGLNILNKAEVNISTAEDPVEINLVGVNQVQINPKADMTFANALRAFLRQDPDIVMVGEIRDLETAEIA
IKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSISLIIAQRLARRLCNNCKTPETLPIEELKSQGFSDQQIGNI
TLFKAVGCEHCTEGYKGRVGIYEVMRITSEISQIIMSGGNSLDIAKASLASGFNSLRLSGLEKAASGVTSLAEINRVTSY

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=141690 PSPO_RS10615 WP_010561749.1 2288534..2290216(+) (pilB) [Pseudoalteromonas spongiae UST010723-006]
ATGAATGTAAACTCTCCGCTTATTCGTAAATGTGTTGCAATGGGGTTTATCGCGCATGAACAAGTAATCTCAGCCGCGAG
TTCAACCACACATATTAGCGAGTTTATCTTAAACAACAGTAAACTTTCATCAAAGCAATTAGCAGGATACTGTTCTTCTT
GGTTTAACTTACCCGTCCTAGATATTGCCACGGTAGAGCCTAGCAGTATACCGCTTGATGATATCAGCGAAGAGCTAGTT
AAAAAACACCATTTGTTACCCTTATCAAAGTGTGGAAGAACTTTATTCTTAGCCACATCTGACCCAACTAATTTTGATGC
CTTCGAAGACTTCGAATTCAACACAGGCCTTCAAACTGAAGCTATAGTTGTTGACTTCAATGCGCTCGAGCAACTTATTG
AAACAATATTTAATAAAACTGATGACTTGGCAATATCAGATGAAGAGTTTGAGCAATTTGGCGATATTGAAGTAACTGAT
ACAGAAGCTAACTTATCTGAAGACAGCGCACCAGATAAAGACGATGCCCCTATCATTGTCTATATCAATAAAATTTTGAT
GGACGCGATACGTAAAGGCGCATCAGATTTACATTTTGAACCTTATGAAAAGTCCTATCGTATCCGTTTTCGCATTGATG
GTATTTTGCATGAGGTAGCATCACCGCCACAAATGCTATCGTCACGCATTTCAGCCCGTGTCAAAGTAATGTCTCGTTTA
GATATTGCAGAAAAACGTAAACCACAAGATGGTCGTATCAAATTAAAAATTACAGCAAAGAAAAGTATCGATTTTCGTGT
CTCCACCCTACCTACCTTGTGGGGAGAAAAAATTGTAATGCGTATTTTGGATTCGAGTAGTGCTATGCTCGGTATTGATG
TGCTTGGTTATGAGCCTGCACAAAAAGCCATGTATTTAGATGCACTTTCACAACCTCAAGGTATGATTTTAGTTACAGGC
CCCACCGGCTCAGGTAAAACAGTATCACTCTACACCGGCTTAAATATTTTAAACAAAGCTGAGGTTAATATTTCCACAGC
CGAAGACCCAGTCGAGATAAACCTTGTCGGTGTTAACCAAGTACAAATAAACCCCAAAGCAGATATGACATTTGCAAACG
CATTACGCGCTTTCCTTCGCCAAGATCCCGACATTGTTATGGTTGGTGAGATACGTGATTTGGAGACCGCTGAAATCGCA
ATAAAAGCAGCGCAAACAGGTCACTTGGTGTTAAGTACGCTACACACAAACTCAGCACCCGAAACTCTTACTCGCTTACT
CAATATGGGCGTACCCGCGTACAATGTTGCAAGTTCTATTAGCTTAATCATTGCACAACGCCTTGCTCGACGCCTTTGCA
ATAATTGTAAAACACCTGAAACCTTACCGATCGAGGAACTCAAGTCGCAGGGCTTCTCTGACCAGCAAATTGGCAATATT
ACGTTATTTAAAGCCGTTGGGTGCGAACATTGTACAGAAGGTTATAAAGGTCGAGTCGGTATCTATGAAGTAATGCGAAT
TACCTCTGAAATATCACAAATCATTATGTCGGGCGGAAATTCTTTGGATATCGCCAAAGCATCATTAGCGAGTGGATTTA
ATAGTTTGCGACTTTCAGGGCTAGAAAAAGCTGCGTCAGGGGTGACTAGTCTTGCCGAGATAAACCGTGTTACCAGTTAC
TAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-451)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(14-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.793

100

0.559
  pilB Acinetobacter baumannii D1279779

58.824

94.107

0.554
  pilB Legionella pneumophila strain ERS1305867

56.667

96.429

0.546
  pilB Vibrio parahaemolyticus RIMD 2210633

52.398

100

0.527
  pilB Vibrio cholerae strain A1552

53.578

97.321

0.521
  pilB Vibrio campbellii strain DS40M4

50.977

100

0.513
  pilF Neisseria gonorrhoeae MS11

47.31

96.25

0.455
  pilB/pilB1 Synechocystis sp. PCC 6803

36.995

100

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.5

100

0.375
  pilF Thermus thermophilus HB27

36.46

100

0.368

Multiple sequence alignment