Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSPO_RS10595 Genome accession   NZ_CP011039
Coordinates   2283901..2285586 (+) Length   561 a.a.
NCBI ID   WP_010561745.1    Uniprot ID   -
Organism   Pseudoalteromonas spongiae UST010723-006     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2278901..2290586
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSPO_RS10570 (PSPO_a2375) ampD 2279431..2279973 (-) 543 WP_010561740.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PSPO_RS10575 (PSPO_a2376) nadC 2280143..2280997 (+) 855 WP_010561741.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PSPO_RS22010 (PSPO_a2377) - 2281387..2281782 (+) 396 WP_010561742.1 type IV pilin protein -
  PSPO_RS10585 (PSPO_a2378) - 2281841..2282953 (+) 1113 WP_010561743.1 methyltransferase family protein -
  PSPO_RS10590 (PSPO_a2380) - 2282944..2283828 (+) 885 WP_010561744.1 hypothetical protein -
  PSPO_RS10595 (PSPO_a2381) pilB 2283901..2285586 (+) 1686 WP_010561745.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSPO_RS10600 (PSPO_a2382) pilC 2285657..2286886 (+) 1230 WP_010561746.1 type II secretion system F family protein Machinery gene
  PSPO_RS10605 (PSPO_a2383) pilD 2286893..2287777 (+) 885 WP_010561747.1 prepilin peptidase Machinery gene
  PSPO_RS10610 (PSPO_a2384) coaE 2287779..2288372 (+) 594 WP_010561748.1 dephospho-CoA kinase -
  PSPO_RS10615 (PSPO_a2387) pilB 2288534..2290216 (+) 1683 WP_010561749.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 61875.94 Da        Isoelectric Point: 4.9014

>NTDB_id=141687 PSPO_RS10595 WP_010561745.1 2283901..2285586(+) (pilB) [Pseudoalteromonas spongiae UST010723-006]
MQLSSPLIRKLESFGYLDRSTLANNHPDTPLIERLCNATQLSAKELAFKAASLFSAPLLELNNFDLDFVPDGKERNEKLI
KKHKVLPIALKGKTLYLASADPTNFDAFEDYEFNTGLQTEVVVVAYDELNTAIDSLFEKSDGIELSDDQLNELSGVTSDI
DNKADEESDVNAKEDDAPIIVYINKILMDAIKRGASDLHFEPYEHVYRIRFRVDGVLHEVANPPIGLESRISARIKVMSQ
LDLAEKRKPQDGRIKLKITAKKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKELYLNALAQPQGMILVT
GPTGSGKTVSLYTGLNILNKPERNISTAEDPVEINLVGINQVQINAKADMTFANALRAFLRQDPDIVMVGEIRDLETAEI
SIKAAQTGHLVLSTLHTNSAPETLTRLMNMGVPSYNIASSVSLIIAQRLARRLCPVCKEPETLPEEELLKLGFTSEQISN
LQLFKAVGCDKCTEGYKGRVGIYEVMPITPKIAEIIMRGGNSLEIAAIAEQEGINNLRKSGLVKAASGVTSITEINRVTS
Y

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=141687 PSPO_RS10595 WP_010561745.1 2283901..2285586(+) (pilB) [Pseudoalteromonas spongiae UST010723-006]
ATGCAACTATCTTCTCCTCTAATTCGAAAACTTGAAAGCTTTGGGTATCTTGACCGCTCAACTTTAGCAAATAATCATCC
CGATACCCCTCTTATTGAACGCCTTTGTAATGCGACACAGTTATCAGCGAAAGAACTGGCGTTTAAAGCTGCTAGCTTGT
TTTCAGCACCTTTACTTGAGTTGAATAATTTTGACCTCGATTTTGTTCCTGACGGGAAAGAAAGAAACGAAAAGCTTATA
AAAAAGCATAAAGTGCTCCCAATCGCATTAAAAGGCAAAACTCTCTATTTAGCTAGTGCGGACCCTACAAATTTTGATGC
GTTTGAAGACTATGAGTTTAATACAGGTTTACAAACTGAAGTGGTGGTGGTTGCATACGATGAACTCAACACCGCAATAG
ATTCATTGTTTGAAAAGAGTGATGGAATAGAGCTAAGCGATGATCAGCTCAATGAGTTGAGTGGCGTAACTTCGGATATA
GATAACAAAGCTGACGAAGAAAGTGATGTAAATGCAAAAGAAGATGACGCTCCAATTATTGTTTACATTAACAAAATTTT
AATGGATGCAATTAAGCGCGGAGCTTCAGATTTACACTTTGAGCCCTATGAACACGTTTACAGAATTCGATTTCGTGTTG
ACGGCGTGTTACATGAAGTTGCTAATCCTCCTATAGGCTTAGAGAGTCGAATTTCAGCACGTATCAAAGTAATGTCTCAA
CTTGATTTAGCAGAAAAGCGAAAGCCACAAGATGGCCGTATTAAATTAAAAATAACGGCTAAAAAAAGCATTGATTTTCG
TGTATCGACTTTACCAACGCTTTGGGGTGAAAAAATTGTAATGCGTATTCTTGATTCATCGAGTGCTATGCTTGGCATTG
ATGTATTAGGGTACGAACCCGAACAAAAAGAGCTTTATTTAAATGCACTTGCACAACCACAAGGCATGATTCTTGTTACA
GGCCCTACAGGCTCAGGTAAAACAGTTTCTCTTTATACAGGTCTAAACATTCTTAACAAACCGGAAAGGAATATTAGTAC
CGCAGAAGATCCTGTAGAAATAAATTTAGTTGGTATTAACCAGGTTCAAATAAATGCTAAGGCAGACATGACCTTTGCCA
ATGCGCTTAGGGCCTTCCTGCGTCAAGATCCGGATATCGTAATGGTAGGTGAGATCCGTGATTTAGAAACCGCTGAAATA
TCTATCAAAGCAGCACAAACTGGTCACTTAGTTTTAAGTACTTTACATACTAACTCCGCACCTGAAACGCTAACGCGTTT
AATGAATATGGGGGTCCCCTCATACAATATCGCAAGCTCTGTAAGCTTAATCATTGCTCAGCGTCTAGCAAGGCGTCTTT
GTCCAGTTTGTAAAGAACCTGAAACTCTTCCTGAAGAAGAGCTATTAAAACTTGGATTCACAAGTGAACAAATATCTAAC
CTACAACTATTTAAGGCTGTAGGTTGCGATAAATGCACAGAAGGTTATAAGGGCCGTGTTGGTATATATGAAGTAATGCC
AATTACACCAAAAATTGCAGAGATTATTATGCGAGGTGGTAACTCTTTGGAAATTGCAGCAATTGCAGAGCAAGAAGGTA
TTAATAATTTGCGAAAATCTGGATTAGTGAAAGCAGCTTCAGGTGTGACAAGTATTACAGAGATAAATCGCGTAACGAGC
TATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(185-452)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

60.194

91.8

0.553
  pilB Acinetobacter baumannii D1279779

57.815

94.652

0.547
  pilB Legionella pneumophila strain ERS1305867

55.888

95.365

0.533
  pilB Vibrio parahaemolyticus RIMD 2210633

54.275

95.9

0.52
  pilB Vibrio cholerae strain A1552

52.768

96.613

0.51
  pilB Vibrio campbellii strain DS40M4

53.084

95.365

0.506
  pilF Neisseria gonorrhoeae MS11

51.154

92.692

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

38.544

100

0.387

Multiple sequence alignment