Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PTRA_RS01870 Genome accession   NZ_CP011034
Coordinates   401161..402387 (-) Length   408 a.a.
NCBI ID   WP_058372468.1    Uniprot ID   A0A0U2WIQ6
Organism   Pseudoalteromonas translucida KMM 520     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 396161..407387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTRA_RS01845 (PTRA_a0405) - 396723..397208 (+) 486 WP_058372464.1 Spy/CpxP family protein refolding chaperone -
  PTRA_RS01850 (PTRA_a0407) yacG 397445..397675 (-) 231 WP_011327088.1 DNA gyrase inhibitor YacG -
  PTRA_RS01855 (PTRA_a0408) pilB 397801..399474 (-) 1674 WP_058372465.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PTRA_RS01860 (PTRA_a0409) coaE 399595..400227 (-) 633 WP_058372466.1 dephospho-CoA kinase -
  PTRA_RS01865 (PTRA_a0410) pilD 400229..401125 (-) 897 WP_058372467.1 prepilin peptidase Machinery gene
  PTRA_RS01870 (PTRA_a0411) pilC 401161..402387 (-) 1227 WP_058372468.1 type II secretion system F family protein Machinery gene
  PTRA_RS01875 (PTRA_a0412) - 402512..403123 (+) 612 WP_058374493.1 hypothetical protein -
  PTRA_RS01880 (PTRA_a0413) - 403104..403535 (-) 432 WP_069188177.1 pilin -
  PTRA_RS01885 (PTRA_a0414) - 403547..403990 (-) 444 WP_058372469.1 pilin -
  PTRA_RS01890 (PTRA_a0416) nadC 404553..405398 (-) 846 WP_058372470.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PTRA_RS01895 (PTRA_a0417) ampD 405698..406252 (+) 555 WP_058372471.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PTRA_RS01900 (PTRA_a0418) ampE 406266..407117 (+) 852 WP_058372472.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44585.66 Da        Isoelectric Point: 9.6986

>NTDB_id=141635 PTRA_RS01870 WP_058372468.1 401161..402387(-) (pilC) [Pseudoalteromonas translucida KMM 520]
MAKQQEKKGQDTFEWVGVSTRGKRLEGELTGSSVALVKAQLRKQGITPSKVKRKAKPLFGARLQKITPKDIAIVTRQIAT
MLMAGVPLIQSIEMIGTGATNKSLGKLMETIADEVKTGQPLSQALRRHPRYFDDLYCDLVASGEQSGALDKIFDRVAIYK
EKSEALKSKIKKAMFYPIAVLVIALIVTSILLIFVVPQFQDIFAGFGAELPAFTLFVIAISEFMQEYWWIILIIIFAFGW
AFKEAHLKSLKLRDATDRAILKLPVIGMILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRYAILEIKAEVS
SGNQMNWAMRNSKIFPDMVIQMVAIGEESGSLDSMLAKVATIYEQEVDDAVDGLSSLLEPMIMAVLGVLVGGLIVAMYLP
IFQLGAVI

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=141635 PTRA_RS01870 WP_058372468.1 401161..402387(-) (pilC) [Pseudoalteromonas translucida KMM 520]
ATGGCTAAACAACAAGAAAAAAAAGGGCAAGATACCTTTGAGTGGGTAGGCGTTAGCACTCGCGGGAAACGTTTAGAAGG
TGAGCTTACAGGTTCTAGTGTTGCGTTAGTAAAAGCCCAACTGCGTAAACAGGGTATTACCCCCTCAAAAGTAAAGCGTA
AAGCCAAACCGTTATTTGGTGCACGCTTACAAAAAATCACCCCTAAAGATATTGCCATAGTCACTCGGCAAATTGCTACC
ATGCTTATGGCGGGTGTACCGTTAATTCAGTCAATTGAAATGATTGGTACTGGAGCAACCAATAAAAGCCTTGGTAAACT
CATGGAAACCATTGCAGATGAAGTAAAGACTGGCCAACCGCTATCGCAAGCACTAAGGCGCCACCCCCGTTATTTTGATG
ATTTATATTGTGATTTAGTGGCATCGGGTGAGCAGTCTGGGGCTTTAGATAAAATATTCGACCGCGTTGCAATTTATAAA
GAAAAGTCTGAAGCGCTTAAATCAAAAATTAAAAAAGCGATGTTTTACCCTATTGCGGTATTAGTTATCGCACTTATTGT
TACCTCTATATTACTAATATTTGTAGTACCGCAATTTCAGGATATTTTTGCCGGATTTGGCGCTGAACTCCCTGCTTTTA
CCTTATTTGTAATTGCTATATCGGAATTTATGCAAGAGTACTGGTGGATAATACTTATAATAATATTTGCCTTTGGTTGG
GCGTTTAAAGAGGCACACCTTAAAAGCTTAAAGCTCCGTGATGCAACAGACAGAGCTATATTAAAGCTCCCTGTTATTGG
TATGATTTTAAACAAAGCTGCTGTTGCCCGTTACGCGCGTACGCTTTCGACTACTTTTGCGGCTGGTGTACCGCTAGTAG
ATGCGCTGGATTCTGCCGCAGGAGCCTCGGGTAATGCGGTTTATCGTTATGCTATTTTAGAAATAAAAGCCGAAGTAAGC
TCGGGTAACCAAATGAACTGGGCAATGCGTAACTCTAAAATATTCCCTGATATGGTAATACAAATGGTGGCTATTGGTGA
AGAGTCGGGATCACTTGATAGCATGTTAGCCAAAGTAGCGACTATTTATGAGCAAGAAGTTGATGATGCGGTAGATGGGC
TCTCAAGCTTGCTAGAACCGATGATTATGGCAGTACTTGGCGTATTGGTTGGTGGTTTAATTGTTGCCATGTACTTGCCT
ATATTCCAACTTGGCGCTGTTATTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-197)

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U2WIQ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

62.374

97.059

0.605
  pilC Acinetobacter baumannii D1279779

55.802

99.265

0.554
  pilC Legionella pneumophila strain ERS1305867

55.668

97.304

0.542
  pilC Acinetobacter baylyi ADP1

54.912

97.304

0.534
  pilC Vibrio cholerae strain A1552

44.361

97.794

0.434
  pilG Neisseria meningitidis 44/76-A

43.257

96.324

0.417
  pilG Neisseria gonorrhoeae MS11

43.003

96.324

0.414
  pilC Vibrio campbellii strain DS40M4

41.562

97.304

0.404

Multiple sequence alignment