Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PTRA_RS01855 Genome accession   NZ_CP011034
Coordinates   397801..399474 (-) Length   557 a.a.
NCBI ID   WP_058372465.1    Uniprot ID   A0A0U2WTZ6
Organism   Pseudoalteromonas translucida KMM 520     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 392801..404474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PTRA_RS01830 (PTRA_a0402) - 393540..394472 (-) 933 WP_058372462.1 alpha/beta fold hydrolase -
  PTRA_RS01835 (PTRA_a0403) - 394476..395891 (-) 1416 WP_058372463.1 ATP-binding protein -
  PTRA_RS01840 (PTRA_a0404) - 395869..396558 (-) 690 WP_058374492.1 response regulator transcription factor -
  PTRA_RS01845 (PTRA_a0405) - 396723..397208 (+) 486 WP_058372464.1 Spy/CpxP family protein refolding chaperone -
  PTRA_RS01850 (PTRA_a0407) yacG 397445..397675 (-) 231 WP_011327088.1 DNA gyrase inhibitor YacG -
  PTRA_RS01855 (PTRA_a0408) pilB 397801..399474 (-) 1674 WP_058372465.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PTRA_RS01860 (PTRA_a0409) coaE 399595..400227 (-) 633 WP_058372466.1 dephospho-CoA kinase -
  PTRA_RS01865 (PTRA_a0410) pilD 400229..401125 (-) 897 WP_058372467.1 prepilin peptidase Machinery gene
  PTRA_RS01870 (PTRA_a0411) pilC 401161..402387 (-) 1227 WP_058372468.1 type II secretion system F family protein Machinery gene
  PTRA_RS01875 (PTRA_a0412) - 402512..403123 (+) 612 WP_058374493.1 hypothetical protein -
  PTRA_RS01880 (PTRA_a0413) - 403104..403535 (-) 432 WP_069188177.1 pilin -
  PTRA_RS01885 (PTRA_a0414) - 403547..403990 (-) 444 WP_058372469.1 pilin -

Sequence

Protein


Download         Length: 557 a.a.        Molecular weight: 62037.50 Da        Isoelectric Point: 5.8045

>NTDB_id=141633 PTRA_RS01855 WP_058372465.1 397801..399474(-) (pilB) [Pseudoalteromonas translucida KMM 520]
MNINSPLLRKFITLGRVDADIVKTKQQEFASTAELIAKCGKIDSKDLAAQCIDLFRVPFFDLKDFNTALIPPELVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEVVVADYVQLDNKIEQLLDATGSLNLSDDEFKEFAELDADI
QKDTTQDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLATKLSARIKVMSRLDIA
EKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALEQPQGMILVTGPTG
SGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIKA
AQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPENLPSEELLRQGFSQQQISEMTLY
APKGCENCTDGYKGRVGIYEVMKITPEIAQIIMRGGNSLEIAEVSLKAGFNNLRLSGIRKAADGMTSLAEINRVTSF

Nucleotide


Download         Length: 1674 bp        

>NTDB_id=141633 PTRA_RS01855 WP_058372465.1 397801..399474(-) (pilB) [Pseudoalteromonas translucida KMM 520]
ATGAATATTAACTCACCGTTATTAAGAAAATTTATTACCCTTGGCCGTGTTGATGCCGATATAGTAAAAACTAAACAGCA
AGAGTTTGCCTCTACTGCTGAGCTTATTGCTAAATGCGGAAAAATCGACAGTAAAGATTTAGCGGCGCAATGCATTGATT
TATTTAGGGTGCCTTTTTTTGATTTAAAAGACTTTAATACCGCACTTATTCCCCCTGAGTTAGTAAAAGAAAAACTGATC
CGTAAGCATCATATTTTGCCTTTAGTACAAAAAGGCAGAAAAGTGTACATTGCCGCCTCTGATCCTACCGATTACGGCGC
ATTTGAAAACTTTGAATTTAGTACCGGTTTGTCTTGTGAAGTGGTGGTAGCTGACTACGTTCAACTCGATAACAAAATAG
AGCAATTGCTTGATGCCACTGGTAGCTTAAATTTAAGTGATGACGAGTTTAAAGAATTTGCCGAACTAGACGCCGATATC
CAAAAAGACACCACTCAAGATGATGATAAAGACGATGCACCAATCATTGTTTACATCAATAAAATATTAATGGATGCAAT
TAAAAAAGGCGCGTCTGATTTACACTTTGAACCTTACGAGCACAAGTACCGAATTCGCTTTAGAATTGACGGCATTTTGC
ACGAAGTAGCCAGCCCACCAAATACTTTAGCAACTAAGCTTTCAGCGCGTATTAAAGTAATGTCGCGCCTTGATATTGCC
GAAAAACGCAAACCGCAAGATGGCCGCATAAAGCTAAAAATAACCGAGCGTAAAAGCATCGATTTTCGGGTAAGCACCAT
GCCCACCTTGTGGGGCGAAAAAATTGTAATGCGTATTCTTGATTCATCTAGCGCCATGCTTGGCATTGATGTATTAGGTT
ACGAACCCGAGCAAAAAAAGCTGTATATGGATGCGCTTGAGCAACCACAAGGGATGATATTAGTAACTGGGCCTACTGGC
TCGGGTAAAACGGTATCGCTTTACACTGGCTTAAATATATTAAACCAGCCAGAGCGTAATATTAGTACCGCCGAAGATCC
GGTAGAAATTAACCTCGAAGGGATTAACCAAGTACAAATTAATATAAAAGCCGATATGACCTTTGCCAACGCATTGCGCG
CATTTTTACGCCAAGATCCCGATGTAGTAATGGTAGGTGAAATACGTGACCTAGAAACCGCTGAAATATCTATTAAAGCG
GCACAAACCGGTCACTTAGTGCTTTCGACCTTACATACTAACTCAGCGCCCGAAACCATTACCCGCTTATTAAACATGGG
CGTACCGGCGTATAACGTAGCAAGTTCAATAAGTTTAATTATAGCTCAGCGCCTAGCGCGTCGCTTATGCCCTAAGTGTA
AAACACCTGAGAATTTACCTAGCGAAGAACTACTGCGCCAAGGTTTTAGCCAGCAACAAATTAGTGAAATGACCCTGTAT
GCCCCTAAAGGTTGCGAAAACTGTACCGATGGTTATAAAGGCCGTGTAGGTATTTACGAAGTAATGAAAATCACCCCAGA
AATAGCACAAATTATAATGCGCGGCGGTAACTCGCTAGAAATAGCTGAAGTGTCGCTTAAAGCCGGATTTAATAACCTGC
GTTTATCGGGTATTCGTAAAGCCGCCGACGGCATGACCTCGTTAGCTGAAATTAATCGGGTAACGAGTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(181-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U2WTZ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.117

93.537

0.553
  pilB Acinetobacter baylyi ADP1

58.721

92.639

0.544
  pilB Legionella pneumophila strain ERS1305867

53.039

97.487

0.517
  pilB Vibrio cholerae strain A1552

52.72

95.691

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

52.533

95.691

0.503
  pilB Vibrio campbellii strain DS40M4

51.873

95.871

0.497
  pilF Neisseria gonorrhoeae MS11

49.52

93.537

0.463
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.324

100

0.386

Multiple sequence alignment