Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PESP_RS14845 Genome accession   NZ_CP011028
Coordinates   3272425..3273651 (+) Length   408 a.a.
NCBI ID   WP_089348724.1    Uniprot ID   -
Organism   Pseudoalteromonas espejiana DSM 9414     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3267425..3278651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PESP_RS14820 (PESP_a3405) - 3268265..3268717 (+) 453 WP_089348720.1 transposase -
  PESP_RS14825 (PESP_a3406) ampE 3268892..3269743 (-) 852 WP_089348721.1 beta-lactamase regulator AmpE -
  PESP_RS14830 (PESP_a3407) ampD 3269757..3270311 (-) 555 WP_089348722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PESP_RS14835 (PESP_a3408) nadC 3270558..3271403 (+) 846 WP_089348723.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PESP_RS14840 (PESP_a3410) - 3271879..3272346 (+) 468 WP_214631482.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PESP_RS14845 (PESP_a3412) pilC 3272425..3273651 (+) 1227 WP_089348724.1 type II secretion system F family protein Machinery gene
  PESP_RS14850 (PESP_a3413) pilD 3273803..3274693 (+) 891 WP_089348725.1 A24 family peptidase Machinery gene
  PESP_RS14855 (PESP_a3414) coaE 3274695..3275336 (+) 642 WP_089348726.1 dephospho-CoA kinase -
  PESP_RS14860 (PESP_a3415) pilB 3275462..3277138 (+) 1677 WP_089348727.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PESP_RS14865 (PESP_a3416) yacG 3277191..3277421 (+) 231 WP_089348728.1 DNA gyrase inhibitor YacG -
  PESP_RS14870 (PESP_a3417) - 3277510..3278001 (-) 492 WP_089348729.1 Spy/CpxP family protein refolding chaperone -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44257.13 Da        Isoelectric Point: 9.8440

>NTDB_id=141576 PESP_RS14845 WP_089348724.1 3272425..3273651(+) (pilC) [Pseudoalteromonas espejiana DSM 9414]
MAKQPDKKKLDTFEWVGVSARGKKLDGNITGTSVALVKAQLRKQGITPSKVKRKAKPLFSSRLQKITPKDIALVTRQIAT
MLMAGVPLIQAIEMIGSGSTNKSVAKLMETIGDEVKAGQPLSQALRRHPRYFDDLYCDLVASGEQSGALDKIFDRVALYK
EKSEALKSKIKKAMFYPIAVLVVALIVTSILLIFVVPQFQDIFNGFGAELPAFTLFVIAISEFMQEYWWIALITVSAAGY
IFKEASLRSLKLRDATDRAILKLPVIGTILNKAAVARYARTLSTTFAAGVPLVDALDSAAGASGNAVYRYAILDIKAEVS
SGNQMNWAMRNSKIFPDMVIQMVAIGEESGSLDGMLAKVATIYEQEVDDAVDGLSSLLEPLIMAVLGVLVGGLIVAMYLP
IFQLGSVI

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=141576 PESP_RS14845 WP_089348724.1 3272425..3273651(+) (pilC) [Pseudoalteromonas espejiana DSM 9414]
ATGGCAAAACAACCGGATAAAAAAAAGTTAGACACTTTCGAGTGGGTTGGTGTAAGTGCACGAGGTAAAAAGCTAGATGG
TAATATTACAGGCACCAGTGTTGCTTTAGTAAAAGCACAACTACGTAAACAAGGCATAACCCCTTCAAAAGTAAAACGTA
AAGCCAAACCTTTATTTAGCTCGCGTCTACAAAAAATCACTCCTAAAGATATTGCGCTAGTTACACGCCAAATAGCCACT
ATGTTAATGGCGGGCGTACCTCTTATTCAGGCTATCGAAATGATAGGATCTGGCTCCACTAACAAAAGCGTAGCCAAATT
GATGGAAACTATTGGCGATGAGGTTAAAGCTGGTCAACCCCTCTCGCAAGCTCTGAGGCGCCATCCTCGCTACTTTGATG
ATTTGTATTGTGATTTAGTCGCATCAGGTGAGCAATCGGGCGCGCTTGATAAAATATTTGACCGCGTAGCCCTTTACAAA
GAAAAATCAGAAGCGCTTAAATCAAAAATTAAAAAAGCCATGTTTTATCCCATTGCTGTTTTAGTGGTTGCCCTCATAGT
TACCTCAATACTACTTATATTCGTAGTACCACAATTTCAAGATATTTTTAATGGCTTTGGTGCAGAACTTCCTGCATTCA
CTCTTTTTGTAATCGCCATATCTGAATTTATGCAAGAATACTGGTGGATTGCGCTAATTACGGTTTCTGCTGCTGGTTAT
ATATTTAAAGAAGCAAGCTTAAGGAGTTTAAAACTCAGAGATGCAACTGATAGAGCCATTTTAAAACTTCCTGTAATTGG
TACGATTTTGAATAAAGCAGCGGTTGCCCGTTACGCACGAACTCTTTCGACCACCTTTGCAGCGGGTGTACCGCTAGTTG
ATGCACTGGACTCAGCTGCAGGGGCTTCAGGCAATGCGGTGTACCGCTATGCTATTTTAGATATAAAAGCAGAGGTTAGC
TCAGGTAACCAAATGAACTGGGCAATGCGTAACTCTAAAATATTCCCTGATATGGTTATTCAAATGGTCGCCATTGGTGA
AGAGTCTGGCTCGCTTGATGGCATGCTAGCCAAGGTAGCGACTATTTACGAACAAGAAGTTGATGATGCCGTTGATGGCC
TTTCTAGCTTATTAGAGCCGCTTATTATGGCAGTACTTGGTGTGCTTGTAGGTGGCTTAATTGTGGCTATGTACTTGCCA
ATTTTCCAGTTGGGCTCTGTAATTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)

Type II secretion system (T2SS), protein F (T2SSF)

(75-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

62.25

98.039

0.61
  pilC Legionella pneumophila strain ERS1305867

56.14

97.794

0.549
  pilC Acinetobacter baylyi ADP1

55.25

98.039

0.542
  pilC Acinetobacter baumannii D1279779

54.75

98.039

0.537
  pilC Vibrio cholerae strain A1552

44.888

98.284

0.441
  pilC Vibrio campbellii strain DS40M4

43.532

98.529

0.429
  pilG Neisseria meningitidis 44/76-A

43.846

95.588

0.419
  pilG Neisseria gonorrhoeae MS11

43.59

95.588

0.417

Multiple sequence alignment