Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PESP_RS14860 Genome accession   NZ_CP011028
Coordinates   3275462..3277138 (+) Length   558 a.a.
NCBI ID   WP_089348727.1    Uniprot ID   -
Organism   Pseudoalteromonas espejiana DSM 9414     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3270462..3282138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PESP_RS14835 (PESP_a3408) nadC 3270558..3271403 (+) 846 WP_089348723.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PESP_RS14840 (PESP_a3410) - 3271879..3272346 (+) 468 WP_214631482.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PESP_RS14845 (PESP_a3412) pilC 3272425..3273651 (+) 1227 WP_089348724.1 type II secretion system F family protein Machinery gene
  PESP_RS14850 (PESP_a3413) pilD 3273803..3274693 (+) 891 WP_089348725.1 A24 family peptidase Machinery gene
  PESP_RS14855 (PESP_a3414) coaE 3274695..3275336 (+) 642 WP_089348726.1 dephospho-CoA kinase -
  PESP_RS14860 (PESP_a3415) pilB 3275462..3277138 (+) 1677 WP_089348727.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PESP_RS14865 (PESP_a3416) yacG 3277191..3277421 (+) 231 WP_089348728.1 DNA gyrase inhibitor YacG -
  PESP_RS14870 (PESP_a3417) - 3277510..3278001 (-) 492 WP_089348729.1 Spy/CpxP family protein refolding chaperone -
  PESP_RS14875 (PESP_a3419) - 3278173..3278862 (+) 690 WP_089348730.1 response regulator transcription factor -
  PESP_RS14880 (PESP_a3421) - 3278840..3280255 (+) 1416 WP_089348731.1 ATP-binding protein -
  PESP_RS14885 (PESP_a3422) - 3280259..3281227 (+) 969 WP_089348732.1 alpha/beta fold hydrolase -
  PESP_RS14890 (PESP_a3423) - 3281193..3282023 (-) 831 WP_089349186.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62056.22 Da        Isoelectric Point: 5.1577

>NTDB_id=141578 PESP_RS14860 WP_089348727.1 3275462..3277138(+) (pilB) [Pseudoalteromonas espejiana DSM 9414]
MNINSPLLRKFITLGRIDADTVKAKQQEFASTAELISKCGRIDSKDLAEQCIDLFRVPYFDLKDFDPAQIPADLVKEKLI
RKHHILPLVQKDRKVYIAASDPTDYGAFENFEFSTGLSCEIVVVDYIQLDNKIEQLLDAAGSLDLSADEFKEFADLDTEG
PKEATPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVANPPNTLASKLSARIKVMSRLDI
AEKRKPQDGRIKLKMTERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEEEQKKLYMDALGQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINPKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCTKCKTPESLPAEELARQGFSEQQINDMTL
YSPKGCESCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLQAGFNNLRLSGLRKASDGLTSLAEINRVTNM

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=141578 PESP_RS14860 WP_089348727.1 3275462..3277138(+) (pilB) [Pseudoalteromonas espejiana DSM 9414]
ATGAATATTAACTCACCGTTATTGAGAAAATTTATAACGCTAGGCCGTATTGATGCCGATACAGTAAAAGCAAAACAGCA
AGAATTTGCCTCAACCGCTGAGCTTATTTCTAAATGTGGAAGAATCGACAGTAAAGACTTAGCTGAGCAATGTATAGATT
TATTTCGCGTACCGTATTTTGATTTAAAAGACTTCGACCCAGCGCAAATACCTGCCGATTTAGTAAAAGAAAAACTAATA
CGCAAGCATCACATTTTACCTCTGGTTCAAAAAGATAGAAAAGTATACATAGCCGCCTCAGATCCAACTGACTACGGCGC
GTTTGAAAACTTTGAATTTAGTACAGGTTTATCGTGCGAAATTGTTGTTGTTGACTACATACAGCTCGACAACAAAATTG
AACAACTACTTGATGCGGCGGGCAGCTTAGATTTAAGCGCAGATGAATTTAAAGAATTTGCCGACTTAGATACTGAAGGC
CCTAAAGAAGCCACCCCAAAAGATGACGATAAAGACGATGCGCCAATCATTGTTTATATCAACAAAATTTTAATGGATGC
CATTAAAAAAGGCGCATCTGATTTACACTTTGAGCCTTATGAGCACAAGTATCGCATTCGCTTTCGTATCGACGGCATTT
TACACGAAGTTGCCAACCCGCCAAATACCTTGGCGAGTAAGCTCTCTGCACGTATAAAAGTAATGTCACGCCTTGATATT
GCTGAAAAACGTAAACCGCAAGATGGTCGTATTAAACTAAAAATGACCGAGCGAAAAAGCATTGATTTTCGTGTAAGTAC
TATGCCGACCCTTTGGGGCGAAAAAATAGTAATGCGTATTTTAGATTCATCAAGCGCTATGCTAGGCATAGATGTTCTTG
GTTATGAAGAAGAGCAAAAAAAGTTATACATGGATGCGCTTGGCCAGCCACAAGGCATGATACTTGTTACCGGCCCTACT
GGCTCGGGTAAAACGGTATCGCTATATACTGGTTTGAATATACTTAACCAACCAGAGCGTAACATTAGTACCGCAGAAGA
CCCTGTTGAGATAAACCTTGAAGGCGTTAACCAAGTACAAATTAACCCTAAAGCAGATATGACCTTTGCAAACGCCTTGC
GTGCGTTTTTACGTCAAGATCCAGACGTTGTAATGGTAGGTGAAATACGTGACCTTGAAACCGCAGAAATTTCTATTAAG
GCGGCGCAAACGGGTCACTTAGTATTATCAACACTACATACCAACTCAGCGCCAGAAACAATTACGCGTTTGTTAAATAT
GGGCGTACCCGCCTATAACGTAGCAAGCTCAATAAGTTTAATTATTGCCCAGCGCTTAGCACGTCGATTATGTACTAAAT
GTAAAACGCCAGAGTCACTGCCAGCAGAAGAACTTGCTCGTCAAGGCTTTAGCGAACAGCAAATAAACGATATGACGCTA
TATTCCCCTAAAGGCTGCGAAAGTTGCACTGATGGCTACAAAGGCCGTGTAGGTATTTACGAGGTAATGCAAATAACCCC
TGAAATAGCGCAAATTATTATGCGCGGTGGTAACTCACTAGAAATAGCCGAAGTATCACTACAGGCTGGGTTTAATAACT
TACGTTTATCGGGTTTACGAAAAGCCTCAGATGGTTTAACGTCTCTGGCTGAAATAAACCGTGTTACCAACATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.81

100

0.568
  pilB Acinetobacter baylyi ADP1

58.218

90.502

0.527
  pilB Legionella pneumophila strain ERS1305867

52.757

97.491

0.514
  pilB Vibrio cholerae strain A1552

52.612

96.057

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

52.256

95.341

0.498
  pilB Vibrio campbellii strain DS40M4

51.316

95.341

0.489
  pilF Neisseria gonorrhoeae MS11

49.042

93.548

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

37.179

100

0.416

Multiple sequence alignment