Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RY45_RS18790 Genome accession   NZ_CP010947
Coordinates   4163652..4164761 (+) Length   369 a.a.
NCBI ID   WP_017411211.1    Uniprot ID   A0A1C2L453
Organism   Aeromonas hydrophila strain AL06-06 isolate Goldfish     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4158652..4169761
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RY45_RS18755 (RY45_18740) - 4159276..4159587 (+) 312 WP_044800788.1 hypothetical protein -
  RY45_RS18760 (RY45_18745) - 4159636..4160055 (-) 420 WP_011707384.1 DUF4426 domain-containing protein -
  RY45_RS18765 (RY45_18750) yggU 4160074..4160373 (-) 300 WP_011707385.1 DUF167 family protein YggU -
  RY45_RS18770 (RY45_18755) - 4160373..4160924 (-) 552 WP_016351969.1 YggT family protein -
  RY45_RS18775 (RY45_18760) proC 4160965..4161789 (-) 825 WP_044800789.1 pyrroline-5-carboxylate reductase -
  RY45_RS18780 (RY45_18765) - 4161848..4162549 (-) 702 WP_044800790.1 YggS family pyridoxal phosphate-dependent enzyme -
  RY45_RS18785 (RY45_18770) pilT 4162589..4163623 (+) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  RY45_RS18790 (RY45_18775) pilU 4163652..4164761 (+) 1110 WP_017411211.1 type IVa pilus ATPase TapU Machinery gene
  RY45_RS18795 (RY45_18780) yaaA 4164793..4165566 (+) 774 WP_044800791.1 peroxide stress protein YaaA -
  RY45_RS18800 (RY45_18785) srmB 4165743..4166966 (-) 1224 WP_044800792.1 ATP-dependent RNA helicase SrmB -
  RY45_RS18805 (RY45_18790) - 4167095..4167805 (+) 711 WP_044800793.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  RY45_RS18810 (RY45_18795) brnQ 4167996..4169303 (+) 1308 WP_080891590.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41035.17 Da        Isoelectric Point: 6.5035

>NTDB_id=140832 RY45_RS18790 WP_017411211.1 4163652..4164761(+) (pilU) [Aeromonas hydrophila strain AL06-06 isolate Goldfish]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIVRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=140832 RY45_RS18790 WP_017411211.1 4163652..4164761(+) (pilU) [Aeromonas hydrophila strain AL06-06 isolate Goldfish]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCACTGGCAGAAGCCCCCCTCGACAAGGCCAGGGCACTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTTGAGCGCTATCTGCATACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGCTGCGCCGCATCGAAACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTTCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAACGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAATCCACCACCCAGGCAGCCATGATCGGTTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAATTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCATTCGACGT
GGCGCTCAAGAGTTCGCTGCGCCAGGCGCCAGACGTGATCCTGATCGGCGAAATTCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACCGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGACGCCGCCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCG
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C2L453

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.259

95.122

0.564
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Acinetobacter baylyi ADP1

39.003

92.412

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36

Multiple sequence alignment