Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VAB027_RS01330 Genome accession   NZ_CP010812
Coordinates   277264..278979 (+) Length   571 a.a.
NCBI ID   WP_032470277.1    Uniprot ID   -
Organism   Vibrio cholerae strain 10432-62     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 272264..283979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VAB027_RS01310 (VAB027_256) pilM 274557..275573 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  VAB027_RS01315 (VAB027_257) pilN 275563..276147 (+) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  VAB027_RS01320 (VAB027_258) pilO 276140..276730 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  VAB027_RS01325 (VAB027_259) pilP 276720..277238 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  VAB027_RS01330 (VAB027_260) pilQ 277264..278979 (+) 1716 WP_032470277.1 type IV pilus secretin PilQ Machinery gene
  VAB027_RS01335 (VAB027_261) aroK 279166..279690 (+) 525 WP_000818616.1 shikimate kinase AroK -
  VAB027_RS01340 (VAB027_262) aroB 279718..280803 (+) 1086 WP_046126013.1 3-dehydroquinate synthase -
  VAB027_RS01345 (VAB027_263) - 280858..282333 (+) 1476 WP_046126014.1 AAA family ATPase -
  VAB027_RS01350 (VAB027_264) - 282403..283236 (+) 834 WP_000744680.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62368.54 Da        Isoelectric Point: 4.6119

>NTDB_id=139980 VAB027_RS01330 WP_032470277.1 277264..278979(+) (pilQ) [Vibrio cholerae strain 10432-62]
MKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSVVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLEELGVRWGVMSTNGSHSVGGSIESNLWQKGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=139980 VAB027_RS01330 WP_032470277.1 277264..278979(+) (pilQ) [Vibrio cholerae strain 10432-62]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTGTGGGTTGGTTTGGCACTTTGTGCTTCTTCCAT
GGTATTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAACAAAGAGAAGGCGGCGGTCT
TAATTGTCGAATTGGCTTCGCCATCGGTGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGCTGAGTATTGAGCTATTGAAA
ACCGATGTGGCGGATGACAAGCTGTATCTGCTCGATGTGAAAGATTTTTCGACCCCTGTAGAGAGCGTGGAAGTATTCCG
TAAAGAGCCAAGCACACAACTTGTTGTGACTGTTGATGGGGAGTTTCAGCACGATTATACCTTGAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTCAAAGCCGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAAAAAGAAGGCAAGCTTATCTCA
ATTAACTTCCAAGATATTCCGGTGCGTAACGTACTGCAGCTGATTGCCGATTATAACGGTTTCAACTTAGTGGTTTCCGA
TTCGGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTCGATAAGCGGGTCGATGGCAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGACTTGCGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGCGATTTAAAATCAGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTGAACATGTTGTCTGAACGAGGTTCGATCAGCATCGACGAACGAACTAACT
CGCTATTGATCCGAGAGCTGCCCGATAATATTGCTGTGATCCGCGAGATCATTGAATCGCTGGATATTCCGGTCAAACAA
GTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGAGGAGCTGGGGGTACGCTGGGGCGTAATGTCGAC
CAATGGTAGCCACTCGGTCGGAGGAAGCATAGAGAGTAACTTATGGCAAAAAGGGTTATTGGCGGATGATGAATTTCCGG
TGGATGAATTCTTAAATGTGAATTTGGCTTCCACCTCTGCCAATGCGTCTTCGATTGCTTTCCAAGTCGCTAAATTGGGC
TCAGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAGGCCGAAATCATCTCTAGCCCTCGTTTGAT
TACCACTAATAAACAGCCTGCCTATATTGAGCAAGGTACTGAGATCCCTTATCTGGAATCGTCTTCCAGTGGTGCCTCTA
CGGTCGCGTTTAAAAAAGCGGTATTGAGCTTGAAGGTGACACCACAAATCACCCCAGATAACCGCTTGGTGCTGGACTTG
AGCGTGACCCAAGACCGACGCGGTGAAACGGTGAAAACCGGCACTGGCGAAGCGGTTTCTATTGATACTCAGCGTATTGG
CACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCGGTAGATA
AGGTGCCACTGCTGGGGGACTTGCCTGTTTTGGGCGCGTTATTCAGACGAACCTATGAGCAAATGGGCAAAAGTGAACTG
CTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

99.825

100

0.998
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

99.825

100

0.998
  pilQ Vibrio campbellii strain DS40M4

78.364

96.322

0.755
  pilQ Legionella pneumophila strain Lp02

42.778

94.571

0.405
  pilQ Legionella pneumophila strain ERS1305867

42.778

94.571

0.405
  comQ Acinetobacter baylyi ADP1

39.336

100

0.394
  pilQ Acinetobacter baumannii D1279779

37.819

100

0.389
  pilQ Acinetobacter baumannii strain A118

37.819

100

0.389
  pilQ Pseudomonas aeruginosa PAK

39.344

96.147

0.378

Multiple sequence alignment