Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VAB027_RS01310 Genome accession   NZ_CP010812
Coordinates   274557..275573 (+) Length   338 a.a.
NCBI ID   WP_000520444.1    Uniprot ID   A0A0F0B833
Organism   Vibrio cholerae strain 10432-62     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 269557..280573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VAB027_RS01295 (VAB027_253) - 270098..270829 (-) 732 WP_000495223.1 glutathione peroxidase -
  VAB027_RS01300 (VAB027_254) oxyR 270969..271862 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  VAB027_RS01305 (VAB027_255) - 271937..274414 (-) 2478 WP_046126012.1 penicillin-binding protein 1A -
  VAB027_RS01310 (VAB027_256) pilM 274557..275573 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  VAB027_RS01315 (VAB027_257) pilN 275563..276147 (+) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  VAB027_RS01320 (VAB027_258) pilO 276140..276730 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  VAB027_RS01325 (VAB027_259) pilP 276720..277238 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  VAB027_RS01330 (VAB027_260) pilQ 277264..278979 (+) 1716 WP_032470277.1 type IV pilus secretin PilQ Machinery gene
  VAB027_RS01335 (VAB027_261) aroK 279166..279690 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37707.44 Da        Isoelectric Point: 7.3328

>NTDB_id=139976 VAB027_RS01310 WP_000520444.1 274557..275573(+) (pilM) [Vibrio cholerae strain 10432-62]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=139976 VAB027_RS01310 WP_000520444.1 274557..275573(+) (pilM) [Vibrio cholerae strain 10432-62]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCTGTTGAAGAGCTGAGCCTCGATTTCGTTAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGCTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCATTGCCTTGTGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F0B833

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

100

100

1
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485

Multiple sequence alignment