Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VAA049_RS01390 Genome accession   NZ_CP010811
Coordinates   289914..291629 (+) Length   571 a.a.
NCBI ID   WP_046121323.1    Uniprot ID   -
Organism   Vibrio cholerae strain 1154-74     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 284914..296629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VAA049_RS01370 (VAA049_264) pilM 287207..288223 (+) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  VAA049_RS01375 (VAA049_265) pilN 288213..288797 (+) 585 WP_046121321.1 PilN domain-containing protein Machinery gene
  VAA049_RS01380 (VAA049_266) pilO 288790..289380 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  VAA049_RS01385 (VAA049_267) pilP 289370..289888 (+) 519 WP_046121322.1 pilus assembly protein PilP Machinery gene
  VAA049_RS01390 (VAA049_268) pilQ 289914..291629 (+) 1716 WP_046121323.1 type IV pilus secretin PilQ Machinery gene
  VAA049_RS01395 (VAA049_269) aroK 291816..292340 (+) 525 WP_000818616.1 shikimate kinase AroK -
  VAA049_RS01400 (VAA049_270) aroB 292368..293453 (+) 1086 WP_046121324.1 3-dehydroquinate synthase -
  VAA049_RS01405 (VAA049_271) - 293508..294983 (+) 1476 WP_001897855.1 AAA family ATPase -
  VAA049_RS01410 (VAA049_272) - 295053..295886 (+) 834 WP_000744680.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62373.56 Da        Isoelectric Point: 4.6758

>NTDB_id=139933 VAA049_RS01390 WP_046121323.1 289914..291629(+) (pilQ) [Vibrio cholerae strain 1154-74]
MKNGLKTYVAQTWLTLWVGFALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLEELGVRWGVMSTNGSHSVGGSIESNLWQKGLLADDEFPVDKFLNVNLASTSANASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=139933 VAA049_RS01390 WP_046121323.1 289914..291629(+) (pilQ) [Vibrio cholerae strain 1154-74]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTGTGGGTTGGTTTTGCACTTTGTGCTTCTTCCAT
GGTGTTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAACAAAGAGAAGGCGGCGGTCT
TAATTGTCGAATTGGCTTCGCCATCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGTTGAGCATTGAACTCCTGAAA
ACCGATGTGGCGGATGACAAGCTGTATCTGCTTGATGTGAAAGATTTTTCGACCCCTGTTGAGAGCGTGGAAGTATTCCG
TAAAGAGCCAAGCACACAACTGGTTGTGACTGTCGATGGGGAGTTTCAGCACGATTATACCTTGAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTCAAAGCTGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAAAAAGAAGGCAAGCTTATTTCA
ATTAACTTCCAAGATATTCCGGTGCGTAACGTACTGCAGCTGATTGCCGATTATAACGGTTTCAACTTAGTGGTTTCCGA
TTCTGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTCGATAAGCGGGTCGATGGCAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGACTTGCGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGTGATTTAAAATCAGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTTAACATGTTGTCTGAACGAGGTTCGATCAGCATCGACGAACGAACTAACT
CGCTATTGATCCGAGAGCTGCCCGATAACATTGCTGTGATCCGCGAGATCATTGAATCGCTGGATATTCCGGTCAAACAA
GTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGAGGAGCTGGGGGTACGCTGGGGCGTGATGTCGAC
CAATGGTAGCCACTCGGTCGGAGGAAGCATAGAGAGTAACTTATGGCAAAAAGGGTTATTGGCGGATGATGAATTTCCGG
TGGATAAATTCTTAAATGTGAATTTGGCTTCCACCTCTGCCAATGCGTCTTCGATTGCTTTCCAAGTCGCTAAATTGGGC
TCGGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAGGCCGAAATCATCTCTAGCCCTCGTTTGAT
TACCACCAATAAACAGCCTGCGTATATTGAGCAAGGTACTGAGATCCCTTATCTGGAATCGTCTTCCAGTGGTGCCTCTA
CGGTCGCGTTTAAAAAAGCGGTATTGAGCTTGAAGGTGACACCACAAATCACCCCCGATAACCGCTTGGTGCTGGACTTG
AGCGTGACCCAAGACCGACGCGGTGAAACGGTGAAAACCGGCACTGGCGAAGCGGTTTCTATTGATACTCAGCGCATTGG
CACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACACAGCATCAATAATTCGGTAGATA
AGGTGCCACTGCTGGGGGACTTGCCTGTTTTGGGCGCGTTATTCAGACGAACCTATGAGCAAATGGGCAAAAGTGAACTG
CTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

99.65

100

0.997
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

99.65

100

0.997
  pilQ Vibrio campbellii strain DS40M4

78.545

96.322

0.757
  pilQ Legionella pneumophila strain ERS1305867

42.778

94.571

0.405
  pilQ Legionella pneumophila strain Lp02

42.778

94.571

0.405
  comQ Acinetobacter baylyi ADP1

39.336

100

0.394
  pilQ Acinetobacter baumannii strain A118

37.543

100

0.385
  pilQ Acinetobacter baumannii D1279779

37.543

100

0.385
  pilQ Pseudomonas aeruginosa PAK

39.344

96.147

0.378

Multiple sequence alignment