Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AS52_RS18915 Genome accession   NZ_CP010586
Coordinates   3737147..3737587 (+) Length   146 a.a.
NCBI ID   WP_033579940.1    Uniprot ID   A0A806UCC3
Organism   Priestia megaterium Q3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3732147..3742587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS52_RS18895 (AS52_03910) - 3732551..3733222 (-) 672 WP_028414985.1 response regulator transcription factor -
  AS52_RS18900 (AS52_03911) - 3733548..3734366 (+) 819 WP_033579942.1 squalene/phytoene synthase family protein -
  AS52_RS18905 (AS52_03912) - 3734402..3736351 (-) 1950 WP_047750005.1 FUSC family protein -
  AS52_RS18910 (AS52_03913) - 3736508..3736768 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  AS52_RS18915 (AS52_03914) nucA/comI 3737147..3737587 (+) 441 WP_033579940.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  AS52_RS18920 (AS52_03915) comJ 3737623..3738030 (+) 408 WP_033579939.1 competence protein ComJ -
  AS52_RS18925 (AS52_03916) - 3738132..3738722 (-) 591 WP_028414990.1 CPBP family intramembrane glutamic endopeptidase -
  AS52_RS18930 (AS52_03917) helD 3739118..3741436 (-) 2319 WP_049166018.1 RNA polymerase recycling motor HelD -
  AS52_RS28130 (AS52_03918) - 3741680..3741805 (-) 126 WP_016765178.1 hypothetical protein -
  AS52_RS18935 (AS52_03919) - 3741945..3742460 (-) 516 WP_049166019.1 enoyl-CoA hydratase/isomerase family protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 16032.88 Da        Isoelectric Point: 4.4411

>NTDB_id=139440 AS52_RS18915 WP_033579940.1 3737147..3737587(+) (nucA/comI) [Priestia megaterium Q3]
MSTYGKKLMASLFIIIACVLGFIGHAAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEAN
REESLKGYPTKTGYDRDEWPMAMCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 441 bp        

>NTDB_id=139440 AS52_RS18915 WP_033579940.1 3737147..3737587(+) (nucA/comI) [Priestia megaterium Q3]
ATGAGTACATACGGGAAAAAATTAATGGCAAGTCTATTCATTATCATTGCATGCGTTTTAGGGTTTATAGGACATGCAGC
GTTTTTAGCTCCTGAAAAAACGGAAGCCGAAGTAAACTATGATGCAACGCTTTATTTTCCACTGGACCGATATCCTGAAA
CGGGTGATCATATTCGCGACGCGATTGCCGCTGGTCACTCTTCCGTTTGCACAATTGATCGCGACGGTGCTGAAGCGAAT
CGGGAAGAATCGCTGAAAGGATACCCAACAAAAACAGGATATGACCGCGATGAATGGCCAATGGCTATGTGCGAAGAAGG
CGGAGCGGGAGCTGATATTCGCTATATTTCACCTTCTGATAACCGCGGAGCGGGCTCATGGGTTGGAAATCAATTAGAAC
AATACCCAGATGGTACACGCGTACAATTTATCGTGCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A806UCC3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

65.079

86.301

0.562

Multiple sequence alignment