Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FORC9_RS02415 Genome accession   NZ_CP009984
Coordinates   485611..487296 (-) Length   561 a.a.
NCBI ID   WP_060530787.1    Uniprot ID   -
Organism   Vibrio vulnificus strain FORC_009     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 480611..492296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC9_RS02385 (FORC9_0418) rplS 481140..481493 (+) 354 WP_060530781.1 50S ribosomal protein L19 -
  FORC9_RS02390 yacG 481733..481927 (-) 195 WP_060530783.1 DNA gyrase inhibitor YacG -
  FORC9_RS02395 (FORC9_0419) zapD 481990..482730 (-) 741 WP_011079546.1 cell division protein ZapD -
  FORC9_RS02400 (FORC9_0420) coaE 482763..483371 (-) 609 WP_060530785.1 dephospho-CoA kinase -
  FORC9_RS02405 (FORC9_0421) pilD 483373..484242 (-) 870 WP_013571138.1 prepilin peptidase Machinery gene
  FORC9_RS02410 (FORC9_0422) pilC 484336..485562 (-) 1227 WP_045618629.1 type II secretion system F family protein Machinery gene
  FORC9_RS02415 (FORC9_0423) pilB 485611..487296 (-) 1686 WP_060530787.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC9_RS02420 (FORC9_0424) - 487301..487750 (-) 450 WP_175546417.1 pilin -
  FORC9_RS02425 (FORC9_0425) nadC 487973..488860 (-) 888 WP_060530792.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC9_RS02430 (FORC9_0426) ampD 488953..489525 (+) 573 WP_060530795.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC9_RS02435 (FORC9_0427) pdhR 489914..490681 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62280.26 Da        Isoelectric Point: 5.4002

>NTDB_id=133463 FORC9_RS02415 WP_060530787.1 485611..487296(-) (pilB) [Vibrio vulnificus strain FORC_009]
MTNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPCTDLNQYDYQELCNQLGLREL
ITRHQALPLQKSNSGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELAN
LVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGMI
LMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADDL
IYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=133463 FORC9_RS02415 WP_060530787.1 485611..487296(-) (pilB) [Vibrio vulnificus strain FORC_009]
ATGACTAACCTTGCCACAATTCTTCGTCAGGCCAATCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAATACA
AGCTTCCGGTGTCACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCGGATGAGTTAACCGAGCAGCTAAGCG
CGATTTTTGGCTTACCATGTACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTACGTGAGCTG
ATCACTCGTCACCAAGCACTGCCACTGCAAAAAAGCAACTCCGGTTTAATACTCGCGGTTGCAGACCCTACCAATATTCA
AGCTGAAGATGATTTCCGTTTTGCCACAGGTGTGCAGGTGGAGATTGTTTTGGCCGATTGCCAAGAGCTGCAAGCGGCAA
TTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAAGCTCTGGTTTAAAAGAGATTCACCAAGATGAGTTGGCGAAT
TTGGTTGAGGTTTCAGATGACGAAGTCGAGTCAATTGAAGACTTAAGCCAAGATGAATCCCCCGTCAGTCGCTTTATCAA
CCAAATCCTGCTGGATGCGGTGCGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGCGAT
TGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAGATC
CTCTCCAAGTTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTCAACCAAGAAACCGCGAT
TGATATGCGCGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGCAGCT
TGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATGATT
TTGATGACGGGCCCAACAGGAAGCGGCAAAACCGTTTCGCTGTACACAGGCTTGAGTTTACTTAACCGGCCAGAAGTGAA
TATCTCCACCGCGGAAGACCCAGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTTTTG
GTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAAACC
GCCGAGATCGCCGTCAAAGCAGCCCAAACCGGTCACCTTGTGCTATCCACCTTACATACCAACTCGGCAGCTGAAACCAT
TGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTAGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGGCTC
GTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGTCTAAAAGCTGACGACTTG
ATTTATCAAGCTGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCATGCC
TTTTGATGAACAGCTTGCAGAGGCGATAGTGAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTTGCTCGGCAAAAAGGCA
TGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGTGTCCTCTAT
TTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

77.005

100

0.77
  pilB Vibrio parahaemolyticus RIMD 2210633

76.071

99.822

0.759
  pilB Vibrio cholerae strain A1552

74.555

100

0.747
  pilB Legionella pneumophila strain ERS1305867

52.827

91.444

0.483
  pilB Acinetobacter baumannii D1279779

51.533

93.048

0.48
  pilB Acinetobacter baylyi ADP1

50.478

93.226

0.471
  pilF Neisseria gonorrhoeae MS11

45.293

100

0.455
  pilF Thermus thermophilus HB27

40.787

99.643

0.406
  pilB/pilB1 Synechocystis sp. PCC 6803

36.888

100

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.474

0.364
  pilB Haemophilus influenzae Rd KW20

41.736

86.275

0.36

Multiple sequence alignment