Detailed information NTG0133461

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: FORC9_RS02405
• Genome accession: NZ_CP009984
• Coordinates: 483373..484242 (-)
• Length: 289 a.a.
• NCBI ID: WP_013571138.1
• Uniprot ID: B7U4R6
• Organism: Vibrio vulnificus strain FORC_009
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 478373..489242

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FORC9_RS02370 (FORC9_0415)	rpsP	479509..479757 (+)	249	WP_011079541.1	30S ribosomal protein S16	-
FORC9_RS02375 (FORC9_0416)	rimM	479773..480321 (+)	549	WP_011079542.1	ribosome maturation factor RimM	-
FORC9_RS02380 (FORC9_0417)	trmD	480349..481098 (+)	750	WP_011079543.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
FORC9_RS02385 (FORC9_0418)	rplS	481140..481493 (+)	354	WP_060530781.1	50S ribosomal protein L19	-
FORC9_RS02390	yacG	481733..481927 (-)	195	WP_060530783.1	DNA gyrase inhibitor YacG	-
FORC9_RS02395 (FORC9_0419)	zapD	481990..482730 (-)	741	WP_011079546.1	cell division protein ZapD	-
FORC9_RS02400 (FORC9_0420)	coaE	482763..483371 (-)	609	WP_060530785.1	dephospho-CoA kinase	-
FORC9_RS02405 (FORC9_0421)	pilD	483373..484242 (-)	870	WP_013571138.1	prepilin peptidase	Machinery gene
FORC9_RS02410 (FORC9_0422)	pilC	484336..485562 (-)	1227	WP_045618629.1	type II secretion system F family protein	Machinery gene
FORC9_RS02415 (FORC9_0423)	pilB	485611..487296 (-)	1686	WP_060530787.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FORC9_RS02420 (FORC9_0424)	-	487301..487750 (-)	450	WP_175546417.1	pilin	-
FORC9_RS02425 (FORC9_0425)	nadC	487973..488860 (-)	888	WP_060530792.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32249.29 Da
• Isoelectric Point: 6.4630

>NTDB_id=133461 FORC9_RS02405 WP_013571138.1 483373..484242(-) (pilD) [Vibrio vulnificus strain FORC_009]
MDALQYYPWLYIGLASLFGLLVGSFLNVVIYRLPKIMELEWRQECAESFPEYNITPPTETLTLSTPRSSCPSCHTPIRVR
DNIPVFSWLALRGKCHHCQTKISARYPFVEALSAFLCGLVAWKFGYAPITVALIGFTLVLIAATFIDLDTMLLPDQLTLP
LTWAGIALALLEISPVSLQDSVIGAMAGYLCLWSVYHLFRLLTGKEGMGYGDFKLLAALGAWLGWQYLPMIILLSSVVGL
IFGLIQLRLQKQGIEMAFPFGPYLAIAGWVALMWGDSLMSWYLNYLMGA

Nucleotide

• Length: 870 bp

>NTDB_id=133461 FORC9_RS02405 WP_013571138.1 483373..484242(-) (pilD) [Vibrio vulnificus strain FORC_009]
ATGGACGCGTTACAATACTACCCTTGGCTTTATATTGGTTTAGCTTCCCTGTTTGGCTTACTGGTAGGCAGCTTTCTTAA
CGTCGTCATCTACCGTCTACCGAAAATTATGGAACTGGAATGGCGCCAAGAGTGTGCAGAGTCCTTTCCTGAATACAACA
TCACACCACCGACAGAAACCCTAACATTAAGCACGCCGCGCTCAAGTTGTCCTTCGTGCCACACCCCAATACGAGTTCGA
GATAATATTCCCGTCTTTAGTTGGTTAGCACTAAGAGGAAAATGTCACCATTGCCAGACGAAAATCAGTGCTCGGTATCC
TTTTGTTGAAGCACTGAGCGCCTTTTTGTGCGGCCTAGTCGCTTGGAAGTTCGGTTATGCACCGATTACCGTAGCGCTGA
TTGGTTTTACTTTGGTGTTGATCGCCGCCACCTTCATCGACTTAGATACCATGCTACTGCCTGATCAGCTCACGCTGCCG
CTGACGTGGGCAGGCATCGCGCTAGCGTTACTAGAGATAAGTCCAGTATCTCTGCAAGATTCAGTGATCGGCGCGATGGC
AGGCTATCTCTGCCTTTGGAGCGTTTATCACCTTTTCCGATTGCTGACAGGGAAAGAAGGCATGGGTTACGGAGACTTTA
AATTGCTTGCGGCACTTGGAGCTTGGCTCGGCTGGCAATATCTACCGATGATCATCTTACTCTCTTCGGTTGTCGGGCTG
ATCTTTGGTCTCATTCAACTCAGATTGCAAAAGCAAGGCATTGAGATGGCTTTTCCATTTGGGCCTTATCTGGCCATTGC
TGGTTGGGTCGCCTTGATGTGGGGAGATAGCTTGATGTCTTGGTATCTCAACTATCTTATGGGGGCATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (137-244)

3D structure

• AlphaFold DB: B7U4R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	77.163	100	0.772
pilD	Vibrio cholerae strain A1552	71.875	99.654	0.716
pilD	Acinetobacter nosocomialis M2	54.348	95.502	0.519
pilD	Acinetobacter baumannii D1279779	54.348	95.502	0.519
pilD	Neisseria gonorrhoeae MS11	48	95.156	0.457