Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   LT40_RS19590 Genome accession   NZ_CP009533
Coordinates   4419485..4420699 (-) Length   404 a.a.
NCBI ID   WP_043192836.1    Uniprot ID   A0A089YYK1
Organism   Pseudomonas rhizosphaerae strain DSM 16299     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4414485..4425699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LT40_RS19565 (LT40_19885) - 4414621..4415922 (-) 1302 WP_043192829.1 NAD(P)/FAD-dependent oxidoreductase -
  LT40_RS19570 (LT40_19890) - 4416251..4416880 (-) 630 WP_043192830.1 DUF1780 domain-containing protein -
  LT40_RS19575 (LT40_19900) - 4416981..4417406 (+) 426 WP_043192831.1 DUF3830 family protein -
  LT40_RS21465 yacG 4417794..4417997 (-) 204 WP_084139849.1 DNA gyrase inhibitor YacG -
  LT40_RS19580 (LT40_19905) coaE 4417994..4418617 (-) 624 WP_043192832.1 dephospho-CoA kinase -
  LT40_RS19585 (LT40_19910) pilD 4418614..4419483 (-) 870 WP_043192835.1 prepilin peptidase Machinery gene
  LT40_RS19590 (LT40_19915) pilC 4419485..4420699 (-) 1215 WP_043192836.1 type II secretion system F family protein Machinery gene
  LT40_RS19595 (LT40_19920) pilB 4420703..4422403 (-) 1701 WP_043192837.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LT40_RS21935 (LT40_19925) - 4422764..4423162 (+) 399 WP_084139850.1 pilin -
  LT40_RS19610 (LT40_19935) - 4423389..4423694 (-) 306 WP_052393484.1 DUF1484 family protein -
  LT40_RS19615 (LT40_19940) - 4424121..4424522 (+) 402 WP_043192838.1 VOC family protein -
  LT40_RS19620 (LT40_19945) - 4424566..4425330 (-) 765 WP_043192840.1 hypothetical protein -
  LT40_RS19625 (LT40_19950) - 4425414..4425659 (+) 246 WP_043192842.1 hypothetical protein -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 43905.81 Da        Isoelectric Point: 10.0120

>NTDB_id=129907 LT40_RS19590 WP_043192836.1 4419485..4420699(-) (pilC) [Pseudomonas rhizosphaerae strain DSM 16299]
MAVKPKVNVYAWEGIDRKGTKMSGELSGHNPALIKAQLRKQGISPGKVKKKGTSLFRKGKRIKPADIALFTRQMSTMMRA
GVPLLQSFDIISEGVDNPNMRKLVDEIKQEVAAGNSFASSLRKKPEYFDDLYCNLVDAGEQAGALETLLDRVATYKEKTE
QLKAKIKKAMTYPIAVVVVAIIVSGILLIKVVPQFKSIFEGFGAELPAFTLMVMGMSDMVQEWYLLLLAGFVGLFFGFKH
AMKRSPKLRDTVDRGLLKVPIVGAILYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNVVFKNAVNRIKADVSTGMQ
LNFSMRSTGVFPTLAIQMTAIGEESGALDDMLDRVATIYEAEVDNMVDSLTSLMEPMIMAVLGVVVGGLVIAMYLPIFQL
GSVV

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=129907 LT40_RS19590 WP_043192836.1 4419485..4420699(-) (pilC) [Pseudomonas rhizosphaerae strain DSM 16299]
ATGGCGGTCAAACCAAAGGTCAACGTTTATGCCTGGGAAGGGATAGACCGCAAAGGCACCAAGATGTCGGGGGAGCTGAG
CGGCCATAATCCAGCGTTGATCAAGGCGCAGTTGCGCAAGCAGGGGATAAGCCCAGGCAAGGTCAAGAAGAAGGGCACCT
CGCTATTCCGAAAAGGCAAACGGATCAAGCCTGCCGACATTGCTCTGTTCACTCGGCAGATGTCGACCATGATGCGTGCT
GGTGTGCCATTATTGCAGTCGTTCGACATCATTTCCGAAGGTGTCGATAACCCTAATATGCGTAAGCTCGTGGACGAGAT
CAAGCAGGAAGTTGCCGCAGGTAACAGCTTCGCCTCGTCACTGCGCAAGAAGCCGGAATACTTCGACGATCTCTACTGCA
ACCTCGTGGATGCCGGTGAGCAGGCTGGTGCCTTGGAAACCTTGCTGGATCGCGTGGCTACTTACAAAGAAAAGACCGAG
CAGCTCAAAGCCAAGATCAAGAAAGCGATGACCTACCCCATCGCTGTAGTAGTTGTTGCCATCATTGTTTCCGGAATCCT
GTTGATCAAGGTAGTGCCGCAGTTCAAATCAATCTTCGAAGGCTTCGGGGCAGAACTTCCAGCGTTTACTTTGATGGTGA
TGGGTATGTCCGACATGGTTCAGGAATGGTACCTACTGCTGCTGGCAGGGTTCGTTGGTTTGTTTTTCGGTTTCAAGCAT
GCCATGAAACGCAGTCCCAAACTGCGTGACACTGTAGATCGCGGGTTGCTCAAAGTGCCGATCGTAGGCGCTATTCTGTA
CAAGTCGGCCGTGGCTCGTTACGCACGCACGCTTTCCACCACATTCGCGGCCGGCGTGCCACTGGTGGATGCGTTGGATT
CGGTGTCAGGCGCTACCGGCAACGTCGTCTTTAAAAACGCCGTAAACCGCATCAAAGCCGACGTTTCCACCGGCATGCAG
CTCAACTTTTCCATGCGCTCGACGGGCGTCTTCCCCACCCTCGCCATTCAAATGACCGCCATCGGCGAAGAATCTGGCGC
GTTGGACGACATGCTCGACCGCGTAGCGACCATTTACGAAGCCGAGGTCGACAACATGGTCGACAGCCTTACCAGCCTCA
TGGAACCCATGATCATGGCCGTACTGGGCGTAGTGGTCGGTGGCCTGGTTATTGCGATGTACCTTCCCATCTTCCAACTC
GGCTCCGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(70-193)

Type II secretion system (T2SS), protein F (T2SSF)

(275-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A089YYK1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

76.942

98.762

0.76
  pilC Acinetobacter baumannii D1279779

58.049

100

0.589
  pilC Acinetobacter baylyi ADP1

59.296

98.515

0.584
  pilC Legionella pneumophila strain ERS1305867

52.369

99.257

0.52
  pilG Neisseria gonorrhoeae MS11

44.975

98.515

0.443
  pilG Neisseria meningitidis 44/76-A

44.975

98.515

0.443
  pilC Vibrio campbellii strain DS40M4

43.176

99.752

0.431
  pilC Vibrio cholerae strain A1552

43.687

98.02

0.428
  pilC Thermus thermophilus HB27

37.656

99.257

0.374

Multiple sequence alignment