Detailed information NTG0129906

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: LT40_RS19585
• Genome accession: NZ_CP009533
• Coordinates: 4418614..4419483 (-)
• Length: 289 a.a.
• NCBI ID: WP_043192835.1
• Uniprot ID: A0A089ZRK3
• Organism: Pseudomonas rhizosphaerae strain DSM 16299
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4413614..4424483

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LT40_RS19565 (LT40_19885)	-	4414621..4415922 (-)	1302	WP_043192829.1	NAD(P)/FAD-dependent oxidoreductase	-
LT40_RS19570 (LT40_19890)	-	4416251..4416880 (-)	630	WP_043192830.1	DUF1780 domain-containing protein	-
LT40_RS19575 (LT40_19900)	-	4416981..4417406 (+)	426	WP_043192831.1	DUF3830 family protein	-
LT40_RS21465	yacG	4417794..4417997 (-)	204	WP_084139849.1	DNA gyrase inhibitor YacG	-
LT40_RS19580 (LT40_19905)	coaE	4417994..4418617 (-)	624	WP_043192832.1	dephospho-CoA kinase	-
LT40_RS19585 (LT40_19910)	pilD	4418614..4419483 (-)	870	WP_043192835.1	prepilin peptidase	Machinery gene
LT40_RS19590 (LT40_19915)	pilC	4419485..4420699 (-)	1215	WP_043192836.1	type II secretion system F family protein	Machinery gene
LT40_RS19595 (LT40_19920)	pilB	4420703..4422403 (-)	1701	WP_043192837.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LT40_RS21935 (LT40_19925)	-	4422764..4423162 (+)	399	WP_084139850.1	pilin	-
LT40_RS19610 (LT40_19935)	-	4423389..4423694 (-)	306	WP_052393484.1	DUF1484 family protein	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 31845.08 Da
• Isoelectric Point: 7.3446

>NTDB_id=129906 LT40_RS19585 WP_043192835.1 4418614..4419483(-) (pilD) [Pseudomonas rhizosphaerae strain DSM 16299]
MLLIEFLASPLALMACALLLGLIIGSFLNVLIHRLPIMLDRDWTTQSREMLGLPSEPKGATYNLLLPHSECPHCAHKIRA
WENIPVVSYLMLGGKCANCRAKISLRYPLVELATGILSAFVVYHFGLGWEAAAFLVLTWGLLAMSLIDADHQLLPDVLVL
PLLWLGLIVNGQGLFTNLYDALWGAVAGYLSLWLVYWVFKLITGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVGA
VLGVLMLRMRNAQTSTPIPFGPYLAIAGWIALLWGDQITTSYMQFAGFR

Nucleotide

• Length: 870 bp

>NTDB_id=129906 LT40_RS19585 WP_043192835.1 4418614..4419483(-) (pilD) [Pseudomonas rhizosphaerae strain DSM 16299]
ATGCTCCTAATTGAATTCCTGGCCAGCCCGCTGGCCCTGATGGCCTGTGCCCTCCTCCTGGGCCTCATCATCGGCAGCTT
CCTCAACGTCCTCATCCACCGCCTTCCCATCATGCTCGACCGCGACTGGACCACGCAGTCCCGGGAGATGCTCGGCTTGC
CGTCCGAGCCCAAGGGCGCTACCTACAACTTGCTGCTCCCCCACTCTGAATGCCCGCACTGCGCGCACAAGATCCGCGCG
TGGGAGAACATTCCAGTGGTGAGTTACCTGATGCTGGGGGGCAAGTGTGCCAATTGCCGGGCGAAGATCAGCTTGCGTTA
TCCGCTGGTCGAGTTGGCCACGGGCATTCTCAGTGCGTTCGTGGTGTATCACTTCGGCCTGGGCTGGGAGGCGGCGGCGT
TTCTGGTGCTGACCTGGGGGCTGCTGGCGATGAGTCTGATTGATGCCGATCATCAGTTGCTGCCGGATGTGCTGGTGCTG
CCGCTGTTGTGGCTGGGGTTGATCGTCAATGGCCAGGGGTTGTTCACCAACCTGTATGACGCGCTGTGGGGCGCGGTGGC
TGGGTATCTCAGCTTATGGCTGGTGTATTGGGTGTTCAAGTTGATCACCGGCAAGGAAGGCATGGGCTACGGCGATTTCA
AGTTGCTGGCCATGCTGGGGGCCTGGGGTGGCTGGCAGATTCTGCCGCTGACCATTCTGTTGTCGTCGCTGGTGGGGGCG
GTGCTGGGGGTGTTGATGTTGCGGATGCGCAACGCGCAAACCAGTACCCCGATCCCGTTCGGGCCTTATCTGGCCATCGC
GGGGTGGATTGCATTGCTGTGGGGTGATCAAATAACCACTTCATACATGCAGTTTGCCGGGTTCCGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

3D structure

• AlphaFold DB: A0A089ZRK3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	51.071	96.886	0.495
pilD	Acinetobacter nosocomialis M2	50.714	96.886	0.491
pilD	Vibrio cholerae strain A1552	51.661	93.772	0.484
pilD	Vibrio campbellii strain DS40M4	49.645	97.578	0.484
pilD	Neisseria gonorrhoeae MS11	50.185	93.772	0.471