Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   EN73_RS10350 Genome accession   NZ_CP007632
Coordinates   187294..188265 (+) Length   323 a.a.
NCBI ID   WP_000250808.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain NGBS572     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 182294..193265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EN73_RS01075 (EN73_01080) rpoC 182992..186642 (+) 3651 WP_000228729.1 DNA-directed RNA polymerase subunit beta' -
  EN73_RS01080 (EN73_01085) - 186756..187121 (+) 366 WP_000285373.1 DUF1033 family protein -
  EN73_RS10350 (EN73_01090) comYA 187294..188265 (+) 972 WP_000250808.1 competence type IV pilus ATPase ComGA Machinery gene
  EN73_RS01085 (EN73_01095) comYB 188159..189202 (+) 1044 WP_223295592.1 competence type IV pilus assembly protein ComGB Machinery gene
  EN73_RS01090 (EN73_01100) comYD 189199..189627 (+) 429 WP_000793381.1 competence type IV pilus minor pilin ComGD Machinery gene
  EN73_RS01095 (EN73_01105) comGE 189599..189898 (+) 300 WP_001867089.1 competence type IV pilus minor pilin ComGE -
  EN73_RS01100 (EN73_01110) comGF 189852..190313 (+) 462 WP_001874060.1 competence type IV pilus minor pilin ComGF -
  EN73_RS01105 (EN73_01115) comGG 190291..190662 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG -
  EN73_RS01110 (EN73_01120) comYH 190777..191751 (+) 975 WP_001008570.1 class I SAM-dependent methyltransferase Machinery gene
  EN73_RS01115 (EN73_01125) - 191783..192976 (+) 1194 WP_000047535.1 acetate kinase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 36779.23 Da        Isoelectric Point: 6.8469

>NTDB_id=121914 EN73_RS10350 WP_000250808.1 187294..188265(+) (comYA) [Streptococcus agalactiae strain NGBS572]
MVQSLAKQVIHQAVEVNAQDIYIIPKGDCYELYMRIDDERRFIDVFEFNRMASLISHFKFVAGMNVGEKRRSQLGSCDYE
LSEGRLVSLRLSSVGDYRGQESLVIRILYSGHQDLKYWFDNIKQMKEVLGTRGLYLFSGPVGSGKTTLMYQLASEVFKNK
QIITIEDPVEIKNDKMLQLQLNEDIGMTYDALIKLSLRHRPDILIIGEIRDQATARAVIRASLTGVMVFSTIHAKSISGV
YDRLIELGVNYQELENSLKLIAYQRLIGGGSLIDFETGNFKKHSSDKWNRQVDILAEEGHISKKQAQVEKIIPQETTESS
PTF

Nucleotide


Download         Length: 972 bp        

>NTDB_id=121914 EN73_RS10350 WP_000250808.1 187294..188265(+) (comYA) [Streptococcus agalactiae strain NGBS572]
ATGGTTCAATCATTAGCAAAGCAAGTCATTCATCAGGCAGTAGAAGTAAATGCTCAAGATATTTATATCATTCCCAAAGG
TGATTGTTATGAACTCTATATGCGTATTGATGATGAAAGGCGGTTTATTGATGTTTTTGAGTTTAATAGGATGGCTAGTC
TTATTAGTCACTTTAAATTTGTGGCAGGCATGAACGTTGGAGAAAAAAGACGAAGTCAATTAGGTTCTTGTGACTATGAA
CTGTCAGAGGGAAGACTGGTTTCATTACGACTATCGAGTGTGGGAGATTATCGTGGTCAAGAATCTTTAGTTATTCGTAT
TTTGTATTCAGGTCATCAGGACTTAAAATATTGGTTTGATAATATAAAGCAAATGAAGGAAGTACTGGGTACAAGAGGGC
TATATCTTTTTTCCGGCCCTGTGGGGAGTGGTAAAACAACTCTCATGTATCAATTAGCTTCAGAAGTATTTAAAAATAAG
CAAATTATCACGATTGAAGATCCGGTAGAAATCAAGAATGACAAGATGTTACAACTCCAATTGAATGAGGATATTGGAAT
GACTTATGATGCTTTAATCAAACTGTCTTTACGGCATCGTCCAGATATTTTAATTATCGGAGAGATTAGAGATCAAGCGA
CGGCCCGTGCTGTTATTCGTGCAAGTTTAACGGGAGTGATGGTTTTTTCTACTATTCATGCTAAAAGTATTTCCGGAGTC
TATGATAGGCTTATAGAATTAGGGGTTAACTATCAAGAGTTAGAAAATAGTCTAAAATTAATAGCATATCAACGTTTAAT
TGGAGGAGGAAGCCTAATTGACTTTGAGACAGGTAACTTTAAAAAACACTCATCAGACAAGTGGAATAGACAAGTGGATA
TCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAAACAACGGAAAGTAGT
CCAACTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

68.489

96.285

0.659
  comYA Streptococcus mutans UA140

68.489

96.285

0.659
  comYA Streptococcus gordonii str. Challis substr. CH1

63.323

98.762

0.625
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

62.701

96.285

0.604
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.736

96.285

0.594
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.613

95.975

0.591
  comGA Lactococcus lactis subsp. cremoris KW2

50.621

99.69

0.505

Multiple sequence alignment