Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACNALX_RS00950 Genome accession   NZ_OZ223210
Coordinates   195618..197285 (+) Length   555 a.a.
NCBI ID   WP_047887157.1    Uniprot ID   A0A0J1H1A6
Organism   MAG: Photobacterium ganghwense isolate daa05ea6-4f3f-4f7c-82d4-3ed073323da5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 190618..202285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNALX_RS00930 pilM 192847..193848 (+) 1002 WP_207103441.1 type IV pilus biogenesis protein PilM -
  ACNALX_RS00935 - 193845..194423 (+) 579 WP_217392190.1 PilN domain-containing protein -
  ACNALX_RS00940 - 194416..195006 (+) 591 WP_415719958.1 type 4a pilus biogenesis protein PilO -
  ACNALX_RS00945 - 195003..195533 (+) 531 WP_047887158.1 pilus assembly protein PilP -
  ACNALX_RS00950 pilQ 195618..197285 (+) 1668 WP_047887157.1 type IV pilus secretin PilQ Machinery gene
  ACNALX_RS00955 aroK 197499..198017 (+) 519 WP_047887156.1 shikimate kinase AroK -
  ACNALX_RS00960 aroB 198038..199120 (+) 1083 WP_415719959.1 3-dehydroquinate synthase -
  ACNALX_RS00965 - 199130..200641 (+) 1512 WP_415719960.1 AAA family ATPase -
  ACNALX_RS00970 - 200749..201576 (+) 828 WP_415719961.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ACNALX_RS00975 - 201649..201918 (-) 270 WP_415719962.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 555 a.a.        Molecular weight: 60696.47 Da        Isoelectric Point: 4.8279

>NTDB_id=1170622 ACNALX_RS00950 WP_047887157.1 195618..197285(+) (pilQ) [MAG: Photobacterium ganghwense isolate daa05ea6-4f3f-4f7c-82d4-3ed073323da5]
MLWALMQSSVSFAAAENRIQAIDFTRSTAGHGVLKVDLANARSVVDMRRAGEKLYVDVHDTEIPEDMIYTLDVKEFATRV
QTIETFRTERGVRLVLSMNGTYAYDYTSKDKVLTVTVEKVAKPSSAKAAPSSISYNSKPISINFQDVPVRNVLQLIAEYN
DFNLVVSDSVQGNLTLRLDDVPWKQVLDIILKSKGLDKREQGSVVMIAPKEELAREEQKQLESKRKEEELASLRSEIIQI
NYAKASELGELLQGSGEGVSMLSPRGSLHIDERTNSLIINDIAESLANIRDIVTALDIPVQQVQIEARVVTVNEGDIDEL
GVRWGMLSRNGNNTIGSSIEGNLVSEGLLDLADNRVDQVGDFLNVNLGATKAGASSIAFQVAKIGDVLLDLELSALQAEQ
KAEIISSPRLMTTNKKMAYIEQGTEIPYLEASSSGAVSVGFKKAVLSLMVTPQITPDGKLVLDLVVTQDKKGDVVKTGTG
EAIAIDTQRIGTQVLVENGETVVLGGIYQHSLTNMVRKVPLLGDLPVLGALFRHKLEELGKRELLIFVTPKIINQ

Nucleotide


Download         Length: 1668 bp        

>NTDB_id=1170622 ACNALX_RS00950 WP_047887157.1 195618..197285(+) (pilQ) [MAG: Photobacterium ganghwense isolate daa05ea6-4f3f-4f7c-82d4-3ed073323da5]
ATGTTATGGGCCCTGATGCAAAGTTCAGTCTCATTTGCTGCCGCAGAAAACCGAATTCAGGCAATCGACTTTACACGCAG
TACGGCCGGGCACGGGGTCCTGAAAGTCGATCTGGCAAACGCCCGTTCCGTGGTCGATATGCGCCGGGCTGGCGAGAAGC
TGTATGTTGATGTGCATGACACGGAGATTCCGGAGGACATGATTTATACCCTCGACGTGAAAGAGTTCGCGACCCGGGTA
CAAACCATCGAAACCTTCCGAACCGAGCGGGGGGTGCGTTTGGTGCTCTCAATGAACGGCACTTATGCCTATGACTATAC
CAGCAAGGATAAAGTGCTGACGGTGACCGTCGAGAAAGTGGCAAAACCAAGCTCAGCCAAGGCCGCGCCGTCATCGATTT
CTTATAACAGCAAGCCCATCTCTATCAACTTCCAGGATGTGCCGGTGCGAAACGTGCTGCAGCTGATCGCTGAGTACAAC
GATTTCAACCTGGTGGTCTCGGATTCGGTACAGGGCAACCTGACGCTGCGGTTGGACGACGTACCATGGAAGCAGGTGCT
GGATATCATTTTGAAATCCAAAGGGCTGGACAAGCGCGAACAGGGCAGTGTGGTCATGATTGCCCCGAAAGAAGAGCTGG
CGCGAGAAGAGCAGAAGCAGCTGGAGTCGAAGCGTAAAGAAGAGGAGCTGGCTTCACTGCGCTCGGAGATTATTCAGATT
AACTACGCCAAGGCATCGGAGCTGGGCGAGCTGCTGCAGGGCAGCGGTGAAGGGGTGAGCATGTTGTCACCGCGTGGCTC
ACTGCATATTGATGAACGAACTAACTCCCTGATCATTAATGATATTGCTGAAAGCTTGGCGAATATCCGCGATATCGTGA
CGGCGCTCGATATCCCAGTGCAGCAGGTCCAAATTGAAGCCCGGGTGGTCACGGTGAACGAAGGTGATATTGACGAGTTG
GGGGTTCGCTGGGGGATGCTGAGTCGTAATGGCAACAATACGATCGGTAGCTCCATTGAAGGCAATTTGGTGTCGGAAGG
GCTGCTTGATCTGGCGGATAACCGGGTGGATCAGGTCGGGGATTTCCTCAATGTTAATTTGGGAGCCACCAAAGCGGGGG
CGTCAAGCATCGCTTTCCAGGTCGCCAAAATCGGCGATGTGTTACTGGATCTGGAATTGTCGGCCCTGCAGGCGGAGCAG
AAAGCGGAAATCATTTCCAGTCCGCGCCTGATGACCACCAATAAAAAAATGGCCTATATCGAGCAGGGAACCGAAATTCC
GTACTTGGAAGCATCATCCAGCGGCGCGGTATCGGTGGGCTTTAAAAAGGCCGTGCTCAGTCTGATGGTGACCCCGCAGA
TTACTCCGGACGGCAAGCTGGTGCTGGATTTGGTCGTTACCCAGGATAAGAAGGGGGATGTCGTAAAAACCGGGACCGGC
GAAGCGATTGCGATTGATACCCAGCGTATCGGGACCCAGGTTCTGGTTGAAAATGGCGAGACCGTTGTTTTGGGGGGAAT
CTACCAGCATTCGCTGACAAACATGGTGCGTAAAGTGCCATTGCTGGGGGATTTACCGGTATTAGGCGCCTTGTTCCGCC
ATAAGCTGGAAGAGCTGGGCAAGCGTGAATTGCTTATTTTTGTGACGCCAAAGATTATTAATCAATAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(162-210)

Bacterial type II and III secretion system protein (Secretin)

(395-553)

Bacterial type II/III secretion system short domain (Secretin_N)

(236-302)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J1H1A6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

61.21

100

0.62
  pilQ Vibrio cholerae strain A1552

61.21

100

0.62
  pilQ Vibrio campbellii strain DS40M4

61.151

100

0.613
  comQ Acinetobacter baylyi ADP1

40.696

100

0.422
  pilQ Legionella pneumophila strain Lp02

41.728

98.018

0.409
  pilQ Legionella pneumophila strain ERS1305867

41.728

98.018

0.409
  pilQ Acinetobacter baumannii D1279779

38.605

100

0.409
  pilQ Acinetobacter baumannii strain A118

38.605

100

0.409
  pilQ Pseudomonas aeruginosa PAK

40.072

100

0.404

Multiple sequence alignment