Detailed information NTG1170214

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: ACLV7Z_RS06665
• Genome accession: NZ_OZ217343
• Coordinates: 1302292..1302942 (-)
• Length: 216 a.a.
• NCBI ID: WP_218773950.1
• Uniprot ID: -
• Organism: Streptococcus mitis isolate S. mitis G22
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1297292..1307942

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLV7Z_RS06645 (SMIG22_13030)	-	1297893..1299077 (-)	1185	WP_218773953.1	hypothetical protein	-
ACLV7Z_RS06650 (SMIG22_13040)	-	1299081..1299668 (-)	588	WP_176139747.1	ABC transporter ATP-binding protein	-
ACLV7Z_RS06655	-	1299701..1299889 (-)	189	WP_218773952.1	hypothetical protein	-
ACLV7Z_RS06660 (SMIG22_13050)	comEC/celB	1300068..1302308 (-)	2241	WP_218773951.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ACLV7Z_RS06665 (SMIG22_13060)	comEA/celA/cilE	1302292..1302942 (-)	651	WP_218773950.1	helix-hairpin-helix domain-containing protein	Machinery gene
ACLV7Z_RS06670 (SMIG22_13070)	-	1303008..1303577 (-)	570	WP_218773949.1	GNAT family N-acetyltransferase	-
ACLV7Z_RS06675 (SMIG22_13080)	-	1303685..1304689 (-)	1005	WP_218773948.1	PhoH family protein	-
ACLV7Z_RS06680 (SMIG22_13090)	-	1304775..1304990 (-)	216	WP_001232084.1	YozE family protein	-
ACLV7Z_RS06685 (SMIG22_13100)	cvfB	1304999..1305853 (-)	855	WP_001095439.1	RNA-binding virulence regulatory protein CvfB	-
ACLV7Z_RS06690 (SMIG22_13110)	frr	1305913..1306470 (-)	558	WP_001262225.1	ribosome recycling factor	-
ACLV7Z_RS06695 (SMIG22_13120)	pyrH	1306478..1307215 (-)	738	WP_031222472.1	UMP kinase	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23270.70 Da
• Isoelectric Point: 6.5093

>NTDB_id=1170214 ACLV7Z_RS06665 WP_218773950.1 1302292..1302942(-) (comEA/celA/cilE) [Streptococcus mitis isolate S. mitis G22]
MEAIIEKIKEYKIIVICTALGLLVGGFFLLKPAPHTPVKETNLQAEVVAVSKDSLIEKEVKKEEKEEPVEQDLITVDIKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAASQQTASGTASSTSKEKKVNLNKA
SLEEIKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=1170214 ACLV7Z_RS06665 WP_218773950.1 1302292..1302942(-) (comEA/celA/cilE) [Streptococcus mitis isolate S. mitis G22]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGCTTTAGGCTTGCTTGTAGGAGGATT
TTTCCTGCTAAAGCCAGCTCCACACACACCTGTCAAAGAAACGAATTTGCAGGCAGAAGTTGTAGCAGTTTCAAAGGATT
CATTAATTGAAAAGGAAGTGAAGAAGGAAGAAAAGGAAGAACCGGTTGAACAAGACCTAATCACAGTAGATATCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGATTTGCCAGTAGGTAGTCGGGTCAATGATGCCGTTCAAAAGGCGGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTCAGTGATGAGGCTCTGGTTTACGTTCCAACTAAGGGAG
AAGAAGCAGCTAGTCAACAGACTGCTTCTGGGACGGCCTCTTCAACAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAGATTAAACAGGTCAAAGGATTAGGTGGCAAACGAGCTCAGGACATTATTGACCATCGTGAGGCAAATGG
GAAATTCAAGTCGGTTGATGAACTTAAGAAGGTCTCTGGCATTGGTGCTAAAACGATAGAAAAGTTAAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-214)

• SLBB domain (SLBB) (76-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus mitis NCTC 12261	95.37	100	0.954
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	94.907	100	0.949
comEA/celA/cilE	Streptococcus pneumoniae Rx1	93.056	100	0.931
comEA/celA/cilE	Streptococcus pneumoniae D39	93.056	100	0.931
comEA/celA/cilE	Streptococcus pneumoniae R6	93.056	100	0.931
comEA/celA/cilE	Streptococcus mitis SK321	92.593	100	0.926
comEA	Lactococcus lactis subsp. cremoris KW2	41.333	100	0.431
comEA	Streptococcus thermophilus LMD-9	37.391	100	0.398
comEA	Bacillus subtilis subsp. subtilis str. 168	39.216	94.444	0.37
comEA	Latilactobacillus sakei subsp. sakei 23K	33.193	100	0.366