Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   AB3221_RS04030 Genome accession   NZ_OZ061323
Coordinates   772727..773602 (+) Length   291 a.a.
NCBI ID   WP_039099523.1    Uniprot ID   -
Organism   Latilactobacillus curvatus isolate Latilactobacillus curvatus CIRM-BIA2781     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 767727..778602
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3221_RS03990 - 767832..768659 (-) 828 WP_054644033.1 LysR family transcriptional regulator -
  AB3221_RS03995 - 768768..769781 (+) 1014 WP_253733849.1 YeiH family protein -
  AB3221_RS04000 - 769872..770111 (-) 240 WP_004270892.1 cytochrome b5 domain-containing protein -
  AB3221_RS04005 - 770306..770641 (+) 336 WP_254670607.1 hypothetical protein -
  AB3221_RS04015 - 770822..771199 (-) 378 WP_111447553.1 hypothetical protein -
  AB3221_RS04020 - 771302..771802 (+) 501 WP_004270893.1 VanZ family protein -
  AB3221_RS04025 - 771892..772623 (+) 732 WP_004270897.1 YebC/PmpR family DNA-binding transcriptional regulator -
  AB3221_RS04030 comGA 772727..773602 (+) 876 WP_039099523.1 competence type IV pilus ATPase ComGA Machinery gene
  AB3221_RS04035 comGB 773592..774593 (+) 1002 WP_259742539.1 type II secretion system F family protein Machinery gene
  AB3221_RS04040 comGC 774590..774898 (+) 309 WP_004270901.1 competence type IV pilus major pilin ComGC Machinery gene
  AB3221_RS04045 - 774963..775322 (+) 360 WP_158022155.1 hypothetical protein -
  AB3221_RS04050 - 775309..775614 (+) 306 WP_056965894.1 hypothetical protein -
  AB3221_RS04055 - 775580..775663 (+) 84 Protein_775 type II secretion system protein -
  AB3221_RS04060 - 775667..776065 (+) 399 WP_240327155.1 ComGF family competence protein -
  AB3221_RS04065 - 776043..776366 (+) 324 WP_039099486.1 hypothetical protein -
  AB3221_RS04070 - 776482..777501 (+) 1020 WP_311732690.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32042.84 Da        Isoelectric Point: 5.5654

>NTDB_id=1165857 AB3221_RS04030 WP_039099523.1 772727..773602(+) (comGA) [Latilactobacillus curvatus isolate Latilactobacillus curvatus CIRM-BIA2781]
MTEVLTTLLQKCATEKAQDLYFRPLSTGWAIIERLATGLKPLQKVTLSEGNQWLNQMKYQAGMDISETRRPQTGRSAIEV
AGELIYLRLATVGDFLNRESLVVRFIYPIGQTYHCDDSQILSRLMQLIQQPGLLLFAGPTGSGKTTSLYYLAEQVMQDKM
VVAIEDPIEIQQPAFLQLQVNEAAGLTYAALLKISLRLRPDTLIIGEIRDLQTAQHAVSAALSGHLVLSTIHARSTTGVM
DRLLDLGISHSQLTACLAGVAYQSLVVDGHNVAAHYELATAQQLEEAVHED

Nucleotide


Download         Length: 876 bp        

>NTDB_id=1165857 AB3221_RS04030 WP_039099523.1 772727..773602(+) (comGA) [Latilactobacillus curvatus isolate Latilactobacillus curvatus CIRM-BIA2781]
ATGACCGAAGTATTAACAACCCTTTTGCAAAAGTGCGCAACGGAAAAAGCGCAGGATCTTTATTTTCGACCACTATCGAC
GGGTTGGGCAATTATTGAACGCTTAGCGACAGGACTAAAGCCGTTACAAAAAGTTACTCTGTCAGAAGGCAATCAATGGC
TCAATCAAATGAAGTATCAAGCGGGGATGGATATCAGTGAAACTCGGCGTCCACAGACCGGCCGTTCAGCGATCGAGGTG
GCAGGCGAATTGATTTATTTACGGTTGGCAACCGTGGGGGATTTTTTGAATCGGGAAAGTTTAGTTGTTCGTTTCATTTA
CCCCATTGGGCAGACTTATCATTGCGACGACTCGCAGATTTTAAGTCGTTTAATGCAGTTAATTCAGCAACCAGGATTAT
TGCTATTTGCCGGTCCAACTGGTTCAGGGAAGACAACAAGTTTGTACTATCTTGCGGAACAAGTCATGCAAGATAAGATG
GTGGTTGCGATTGAAGATCCGATAGAGATTCAACAGCCAGCGTTCTTACAGTTACAGGTGAATGAAGCCGCAGGGTTAAC
CTATGCAGCATTGTTGAAAATCAGTTTGCGATTACGGCCTGATACCTTAATTATTGGTGAGATTCGCGACTTACAGACGG
CCCAACACGCGGTTTCTGCGGCGTTGAGCGGTCACTTGGTACTAAGTACGATTCACGCACGAAGTACAACGGGGGTGATG
GACCGTCTATTAGATCTTGGAATTAGCCACTCACAATTAACCGCTTGTTTAGCGGGTGTTGCCTATCAATCACTAGTGGT
CGACGGCCACAATGTAGCGGCCCACTATGAGTTAGCAACTGCACAACAATTGGAGGAGGCTGTTCATGAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

69.892

95.876

0.67
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

43.015

93.471

0.402
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

42.593

92.784

0.395
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae R6

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae D39

41.573

91.753

0.381
  comGA Lactococcus lactis subsp. cremoris KW2

41.603

90.034

0.375
  comYA Streptococcus mutans UA140

37.113

100

0.371
  comYA Streptococcus mutans UA159

37.113

100

0.371
  comYA Streptococcus gordonii str. Challis substr. CH1

39.194

93.814

0.368

Multiple sequence alignment