Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   U5A08_RS04145 Genome accession   NZ_OY817130
Coordinates   935339..936547 (-) Length   402 a.a.
NCBI ID   WP_321850450.1    Uniprot ID   -
Organism   Pseudomonas paraveronii strain FLM 11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 930339..941547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U5A08_RS04110 - 931214..931963 (+) 750 WP_253393352.1 TIM barrel protein -
  U5A08_RS04115 - 932217..932684 (+) 468 WP_321850447.1 FAD/FMN-containing dehydrogenase -
  U5A08_RS04120 - 932681..933370 (+) 690 WP_253393354.1 energy-coupling factor ABC transporter permease -
  U5A08_RS04125 - 933447..933662 (+) 216 WP_247396658.1 hypothetical protein -
  U5A08_RS04130 yacG 933659..933859 (-) 201 WP_253393355.1 DNA gyrase inhibitor YacG -
  U5A08_RS04135 coaE 933856..934479 (-) 624 WP_253393356.1 dephospho-CoA kinase -
  U5A08_RS04140 pilD 934476..935342 (-) 867 WP_321850449.1 A24 family peptidase Machinery gene
  U5A08_RS04145 pilC 935339..936547 (-) 1209 WP_321850450.1 type II secretion system F family protein Machinery gene
  U5A08_RS04150 - 936749..937162 (+) 414 WP_321852171.1 pilin -
  U5A08_RS04155 - 937198..938607 (+) 1410 WP_321850451.1 O-antigen ligase family protein -
  U5A08_RS04165 - 938856..939161 (+) 306 WP_253393360.1 DUF6388 family protein -
  U5A08_RS04170 nadC 939205..940053 (-) 849 WP_321850453.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43378.69 Da        Isoelectric Point: 10.0171

>NTDB_id=1162036 U5A08_RS04145 WP_321850450.1 935339..936547(-) (pilC) [Pseudomonas paraveronii strain FLM 11]
MNNASMTYAWEGINRQGRRVSGQAIGHDLALIKAQLRQQGICPGRVRKQSRGLPSLTPPIKPADIALFTRQLATLLKAGI
PLLQAFDVISEGFDNRHMCKLVNGLKQEIGAGNNLASALRKQPRYFNDLYCNLVAAGEQAGALETLLERVAVHLEKSERL
KARIKKAMTYPIAVIAVASLVSSLLLIHVVPQFQNLFAGVDSKLPGFTLHVIALSEFLQQAWWMLALSLGAGGFGVRQAY
RASPGFRHWLDAGLLKAPLAGTLLKKSAVARYARTLSTTFAAGVPLVQALDSVAAAAGNQLFKQAIEHMRHDVSTGMPLN
RSMASSGLFPGMAIQMTAIGEESGTLEHMLEKVASHYESDVDNLVDHLTSLMEPLIMVVLGGIVGALVIALYLPVFQLGT
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=1162036 U5A08_RS04145 WP_321850450.1 935339..936547(-) (pilC) [Pseudomonas paraveronii strain FLM 11]
ATGAACAACGCTTCCATGACATACGCCTGGGAAGGCATCAACCGCCAGGGACGCAGGGTGAGCGGGCAAGCCATCGGCCA
CGACCTCGCGCTGATCAAGGCACAACTGCGCCAGCAAGGGATTTGCCCAGGCCGCGTGCGCAAGCAATCTCGCGGCCTGC
CGAGCCTCACTCCACCGATCAAACCGGCGGATATTGCCCTGTTCACCCGCCAGTTGGCGACGCTGCTGAAGGCCGGCATT
CCGTTACTGCAAGCCTTCGACGTCATCAGCGAAGGCTTCGACAACCGCCACATGTGCAAGCTGGTAAATGGCTTGAAACA
GGAGATCGGCGCCGGCAATAACCTGGCGTCGGCATTGCGCAAACAGCCGCGTTACTTCAATGACTTGTACTGCAACCTGG
TGGCCGCGGGTGAGCAGGCCGGCGCCCTGGAAACCCTGTTGGAGCGAGTGGCGGTTCACCTGGAAAAGAGCGAACGGCTC
AAGGCCAGGATCAAGAAAGCCATGACCTACCCCATCGCCGTCATCGCCGTCGCCAGCCTTGTCAGCAGTCTCTTGCTGAT
CCATGTGGTGCCGCAGTTCCAGAACCTGTTCGCCGGAGTCGACAGCAAGCTGCCCGGTTTTACCCTGCATGTGATTGCCT
TGTCCGAGTTCCTGCAACAAGCCTGGTGGATGCTGGCGCTCAGCCTCGGCGCGGGAGGTTTCGGGGTGCGCCAGGCCTAT
CGCGCATCGCCCGGTTTTCGCCATTGGCTGGATGCCGGTTTGTTGAAAGCGCCCCTGGCAGGCACACTGCTGAAAAAGTC
GGCAGTCGCCCGCTACGCCCGCACGCTTTCGACTACGTTCGCGGCCGGAGTGCCGTTGGTGCAGGCCCTGGACTCAGTGG
CCGCCGCTGCCGGCAACCAGCTATTCAAACAGGCTATCGAGCATATGCGTCACGATGTATCCACAGGCATGCCGTTGAAC
CGATCCATGGCCTCCAGCGGCCTGTTTCCCGGCATGGCGATCCAGATGACGGCGATTGGTGAAGAATCCGGCACCCTGGA
GCACATGCTGGAAAAGGTCGCGAGCCACTACGAGTCTGACGTCGACAATCTGGTCGACCACCTCACCAGCCTGATGGAAC
CGCTGATCATGGTGGTGCTCGGAGGGATTGTCGGCGCCTTGGTAATCGCCCTGTACCTGCCGGTCTTCCAACTCGGTACG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

60.914

98.01

0.597
  pilC Acinetobacter baylyi ADP1

50.758

98.507

0.5
  pilC Acinetobacter baumannii D1279779

48.737

98.507

0.48
  pilC Legionella pneumophila strain ERS1305867

48.363

98.756

0.478
  pilC Vibrio cholerae strain A1552

39.541

97.512

0.386
  pilG Neisseria gonorrhoeae MS11

39.737

94.527

0.376
  pilC Thermus thermophilus HB27

37.656

99.751

0.376
  pilC Vibrio campbellii strain DS40M4

38.01

97.512

0.371
  pilG Neisseria meningitidis 44/76-A

38.947

94.527

0.368

Multiple sequence alignment