Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   U5A05_RS04110 Genome accession   NZ_OY817088
Coordinates   855658..856875 (-) Length   405 a.a.
NCBI ID   WP_321835816.1    Uniprot ID   -
Organism   Pseudomonas kulmbachensis strain FLM 004-28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 850658..861875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U5A05_RS04075 - 850812..852113 (-) 1302 WP_321835814.1 NAD(P)/FAD-dependent oxidoreductase -
  U5A05_RS04080 - 852179..852352 (-) 174 WP_261740923.1 DUF3094 domain-containing protein -
  U5A05_RS04085 - 852443..853072 (-) 630 WP_261740924.1 DUF1780 domain-containing protein -
  U5A05_RS04090 - 853182..853871 (+) 690 WP_261740925.1 energy-coupling factor ABC transporter permease -
  U5A05_RS04095 yacG 853970..854173 (-) 204 WP_261740926.1 DNA gyrase inhibitor YacG -
  U5A05_RS04100 coaE 854170..854793 (-) 624 WP_261740927.1 dephospho-CoA kinase -
  U5A05_RS04105 pilD 854795..855655 (-) 861 WP_321835815.1 A24 family peptidase Machinery gene
  U5A05_RS04110 pilC 855658..856875 (-) 1218 WP_321835816.1 type II secretion system F family protein Machinery gene
  U5A05_RS04115 - 857089..857496 (+) 408 WP_321835817.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  U5A05_RS04125 - 857734..859224 (+) 1491 WP_321835818.1 AlkA N-terminal domain-containing protein -
  U5A05_RS04130 - 859227..859724 (+) 498 WP_261740931.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  U5A05_RS04135 nadC 859842..860690 (-) 849 WP_321835819.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44147.19 Da        Isoelectric Point: 9.6266

>NTDB_id=1162018 U5A05_RS04110 WP_321835816.1 855658..856875(-) (pilC) [Pseudomonas kulmbachensis strain FLM 004-28]
MATQAVKTRLYRWQGTDLKGARISGQITADSHVMIRVQLRRQGITVSKIRCHTNNQCPGAKSISAVDITLFTRQLATLIN
AGIPLLQALEVIANGFEQRAMRQLVTELKQDIGAGTSLAAALRKKPAYFDLLFCSLVEAGELAGALDVLLLRVATYKEKT
ETLKKKIRKAMTYPIAVLLVALAVSSILLIEVVPQFQGMFSSFDAQLPVFTLWVIALSEVLQTHGLWLLGFFCVTLACLR
HRYMHSALLRQRADRWLLKLPVIGNLLHLSAIARFARTLSTTIAAGVPLLEALNSVASATGNSLYKEAVLRLKQQVATGS
QIHVAMGATGVFPGMAIQMTAIGEESGTLDDMLDHVATYYESEVDTLVDSLTTLMEPAIMVVLGIMVGGLVIAMYLPIFK
LGQVI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1162018 U5A05_RS04110 WP_321835816.1 855658..856875(-) (pilC) [Pseudomonas kulmbachensis strain FLM 004-28]
ATGGCAACACAGGCCGTTAAAACCAGGCTTTATCGATGGCAGGGCACTGATCTCAAGGGGGCTCGAATCAGTGGTCAAAT
CACCGCAGACAGTCACGTAATGATCAGGGTCCAGCTGCGCCGCCAAGGTATTACCGTTAGTAAAATCCGCTGCCATACAA
ACAATCAGTGTCCAGGCGCCAAATCCATCAGTGCTGTGGATATCACCCTGTTCACCCGTCAGTTGGCCACCCTGATCAAC
GCCGGTATCCCGCTACTGCAAGCCCTGGAGGTTATCGCCAACGGTTTCGAGCAGCGCGCAATGCGCCAACTGGTGACCGA
ACTCAAACAGGACATCGGTGCAGGCACCAGCCTGGCGGCTGCGCTGCGCAAAAAACCGGCATATTTTGACCTGCTGTTTT
GCAGTCTCGTTGAAGCAGGGGAGCTGGCTGGGGCTCTGGATGTGTTGCTGCTGCGGGTGGCCACCTACAAGGAAAAAACC
GAAACCCTGAAAAAGAAAATCAGAAAGGCCATGACCTACCCCATCGCCGTACTGCTGGTGGCATTAGCGGTGAGCAGCAT
TCTGTTGATCGAAGTGGTACCGCAATTTCAGGGCATGTTTTCCTCATTCGACGCACAATTACCCGTGTTCACGTTGTGGG
TAATCGCCCTGTCAGAGGTACTGCAAACACACGGCCTGTGGCTGCTGGGATTTTTTTGCGTCACGCTTGCCTGTCTGCGC
CATCGCTATATGCACTCCGCCCTTCTGCGCCAGCGCGCTGATCGATGGCTACTGAAGTTGCCCGTCATCGGCAACCTGTT
GCACTTGTCTGCGATAGCCCGCTTCGCCCGCACGCTGTCGACCACCATCGCGGCCGGAGTGCCACTGCTTGAGGCGCTGA
ATTCTGTGGCCAGCGCTACTGGCAATAGCCTGTACAAAGAAGCGGTGCTACGGCTCAAACAACAGGTGGCGACGGGCAGC
CAGATCCATGTGGCCATGGGTGCTACCGGGGTGTTCCCTGGCATGGCGATTCAAATGACAGCAATTGGCGAAGAATCCGG
CACACTGGACGACATGCTTGACCACGTAGCCACCTATTATGAAAGCGAGGTCGACACTCTGGTGGATAGCCTCACAACCT
TGATGGAGCCCGCAATCATGGTGGTACTTGGGATCATGGTGGGCGGGCTGGTAATCGCCATGTATTTACCGATTTTCAAA
CTGGGCCAAGTGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.074

100

0.541
  pilC Legionella pneumophila strain ERS1305867

49.875

98.519

0.491
  pilC Acinetobacter baumannii D1279779

50.126

98.025

0.491
  pilC Acinetobacter baylyi ADP1

50

98.272

0.491
  pilC Vibrio cholerae strain A1552

41.481

100

0.415
  pilC Vibrio campbellii strain DS40M4

38.916

100

0.39
  pilG Neisseria meningitidis 44/76-A

40.206

95.802

0.385
  pilG Neisseria gonorrhoeae MS11

40.206

95.802

0.385
  pilC Thermus thermophilus HB27

36.634

99.753

0.365

Multiple sequence alignment