Detailed information NTG1162017

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: U5A05_RS04105
• Genome accession: NZ_OY817088
• Coordinates: 854795..855655 (-)
• Length: 286 a.a.
• NCBI ID: WP_321835815.1
• Uniprot ID: -
• Organism: Pseudomonas kulmbachensis strain FLM 004-28
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 849795..860655

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U5A05_RS04075	-	850812..852113 (-)	1302	WP_321835814.1	NAD(P)/FAD-dependent oxidoreductase	-
U5A05_RS04080	-	852179..852352 (-)	174	WP_261740923.1	DUF3094 domain-containing protein	-
U5A05_RS04085	-	852443..853072 (-)	630	WP_261740924.1	DUF1780 domain-containing protein	-
U5A05_RS04090	-	853182..853871 (+)	690	WP_261740925.1	energy-coupling factor ABC transporter permease	-
U5A05_RS04095	yacG	853970..854173 (-)	204	WP_261740926.1	DNA gyrase inhibitor YacG	-
U5A05_RS04100	coaE	854170..854793 (-)	624	WP_261740927.1	dephospho-CoA kinase	-
U5A05_RS04105	pilD	854795..855655 (-)	861	WP_321835815.1	A24 family peptidase	Machinery gene
U5A05_RS04110	pilC	855658..856875 (-)	1218	WP_321835816.1	type II secretion system F family protein	Machinery gene
U5A05_RS04115	-	857089..857496 (+)	408	WP_321835817.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
U5A05_RS04125	-	857734..859224 (+)	1491	WP_321835818.1	AlkA N-terminal domain-containing protein	-
U5A05_RS04130	-	859227..859724 (+)	498	WP_261740931.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31578.61 Da
• Isoelectric Point: 8.9596

>NTDB_id=1162017 U5A05_RS04105 WP_321835815.1 854795..855655(-) (pilD) [Pseudomonas kulmbachensis strain FLM 004-28]
MIDFSNFHPDAFIACALLLGLLAGSFINVLAWRLPKMLEQDWQSQARELLDLPEPAKRPVYNLRHPRSHCPACTHPLRLR
DNIPVLSYLFLRGKCAHCKTAISLRYPFTELACAVLSGVVAWHFAFGAQAGWMLLLTWGLLAISLIDAKHQIVPDMLVFP
LLWLGLLLNSFDLFTSLPQAVWGAALGYASLWSVFWLFKLITGKEGMGYGDFKLLAMLGAWGGVAVLPLTILFSSLLGAL
VGLGLLALRRAKTSTAIPFAPYLAIAGWIALLWGGQITSFYWPISV

Nucleotide

• Length: 861 bp

>NTDB_id=1162017 U5A05_RS04105 WP_321835815.1 854795..855655(-) (pilD) [Pseudomonas kulmbachensis strain FLM 004-28]
ATGATTGATTTCTCCAATTTTCATCCTGATGCATTCATTGCCTGTGCCCTACTATTGGGCTTGTTGGCGGGCAGCTTTAT
CAATGTCCTGGCGTGGCGTTTGCCGAAAATGCTCGAACAGGACTGGCAGTCTCAGGCCCGCGAGTTGCTGGATCTACCCG
AGCCCGCCAAGCGCCCGGTGTATAACTTGAGACACCCTCGCTCCCACTGCCCCGCGTGCACGCATCCATTGCGCCTGCGG
GACAACATTCCAGTGCTGAGCTATCTTTTTTTACGGGGCAAGTGCGCGCACTGCAAAACCGCTATCAGCCTGCGCTACCC
CTTCACCGAACTGGCCTGCGCCGTATTGTCGGGCGTTGTAGCCTGGCATTTCGCCTTTGGTGCGCAAGCGGGCTGGATGC
TCCTGCTGACCTGGGGGCTTCTGGCCATTAGCCTGATTGATGCCAAACATCAAATCGTGCCCGACATGCTGGTGTTTCCC
CTGCTCTGGCTGGGGTTGTTGCTCAACAGTTTCGATCTTTTCACCAGCCTGCCCCAAGCCGTATGGGGCGCGGCATTAGG
CTATGCAAGCCTGTGGTCGGTGTTCTGGCTATTCAAACTGATCACCGGCAAGGAAGGCATGGGCTATGGCGACTTCAAGT
TACTGGCCATGCTCGGGGCTTGGGGCGGGGTCGCGGTTCTGCCATTGACCATCCTGTTTTCTTCGCTGCTGGGTGCGCTG
GTGGGGTTGGGCCTTTTGGCTTTGCGCCGCGCCAAAACGTCCACCGCCATTCCCTTTGCACCGTATCTGGCAATTGCCGG
CTGGATTGCATTGCTCTGGGGTGGTCAAATAACCAGCTTCTATTGGCCGATTTCGGTTTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

• Type IV leader peptidase family (Peptidase_A24) (135-243)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	50.923	94.755	0.483
pilD	Vibrio cholerae strain A1552	48.239	99.301	0.479
pilD	Acinetobacter nosocomialis M2	50.185	94.755	0.476
pilD	Vibrio campbellii strain DS40M4	45.614	99.65	0.455
pilD	Neisseria gonorrhoeae MS11	45.185	94.406	0.427