Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLMBN_RS09320 Genome accession   NZ_OY760626
Coordinates   1953893..1955032 (-) Length   379 a.a.
NCBI ID   WP_041609767.1    Uniprot ID   -
Organism   MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1948893..1960032
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLMBN_RS09290 (U2939_RS09260) - 1949386..1949844 (-) 459 WP_321405989.1 YccF domain-containing protein -
  ACLMBN_RS09295 (U2939_RS09265) - 1949881..1950771 (-) 891 WP_321405991.1 LysR family transcriptional regulator -
  ACLMBN_RS09300 (U2939_RS09270) - 1950873..1951514 (+) 642 WP_321405993.1 NAD(P)-dependent oxidoreductase -
  ACLMBN_RS09305 (U2939_RS09275) - 1951614..1952225 (+) 612 WP_321405995.1 YigZ family protein -
  ACLMBN_RS09310 (U2939_RS09280) rsuA 1952249..1952944 (+) 696 WP_321405997.1 16S rRNA pseudouridine(516) synthase RsuA -
  ACLMBN_RS09315 (U2939_RS09285) - 1952941..1953825 (-) 885 WP_321405999.1 DMT family transporter -
  ACLMBN_RS09320 (U2939_RS09290) pilU 1953893..1955032 (-) 1140 WP_041609767.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLMBN_RS09325 (U2939_RS09295) - 1955229..1956098 (+) 870 WP_321406001.1 fructosamine kinase family protein -
  ACLMBN_RS09330 (U2939_RS09300) - 1956170..1956379 (+) 210 WP_321406003.1 YaeP family protein -
  ACLMBN_RS09335 (U2939_RS09305) - 1956423..1957781 (+) 1359 WP_321406005.1 MATE family efflux transporter -
  ACLMBN_RS09340 (U2939_RS09310) hxpB 1957914..1958564 (+) 651 WP_321406007.1 hexitol phosphatase HxpB -
  ACLMBN_RS09345 (U2939_RS09315) aroG 1958627..1959676 (-) 1050 WP_321406009.1 3-deoxy-7-phosphoheptulonate synthase AroG -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42542.05 Da        Isoelectric Point: 5.9816

>NTDB_id=1161511 ACLMBN_RS09320 WP_041609767.1 1953893..1955032(-) (pilU) [MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d]
MEFLDMLIKLAYENGSDLYLSTGAVPSIKFNGVLTPIQQLPMKPGEVAAIADDVMDAEQKVIFEKELEMNLALSLPRVGR
FRVNIFRQRNEISIVARNIKLDIPTFEELKLPPVLLDVIMQKRGLVLFVGGTGSGKSTSLAALIDHRNRNTSGHIITIED
PIEYIHPHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLAISTLHANNANQALDRI
INFFPEERHAQLMNDLGNNLKAFVSQRLVKTVDGKRHAAVEILLGTATIQDMIRRGDFSNIKEIMEKSVNLGMKTFDQCL
FELYCEGIIDEEEALRNADSLNNLKLKIKLHDENGALEIKGPALNWELDPVKRDEPGFL

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1161511 ACLMBN_RS09320 WP_041609767.1 1953893..1955032(-) (pilU) [MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d]
ATGGAATTTCTGGATATGCTGATTAAGCTGGCCTATGAAAATGGCTCGGACTTATATCTGTCAACAGGAGCGGTTCCCAG
TATTAAATTTAATGGTGTGCTGACACCAATCCAGCAATTACCGATGAAACCGGGCGAAGTGGCAGCTATTGCCGATGATG
TCATGGATGCGGAACAAAAGGTCATTTTCGAGAAAGAACTGGAAATGAATCTGGCACTGTCCTTACCCCGGGTTGGTCGT
TTCCGCGTAAATATTTTCCGTCAGCGTAATGAGATATCGATTGTAGCCCGTAATATCAAACTTGATATTCCTACTTTTGA
AGAGTTAAAGCTGCCACCCGTGTTGCTGGACGTCATCATGCAAAAACGCGGGCTGGTGCTGTTTGTCGGGGGAACAGGCT
CAGGTAAATCAACTTCGTTAGCTGCGCTCATCGATCATCGTAACCGGAATACCAGTGGTCATATCATTACCATTGAAGAT
CCTATCGAATATATTCATCCGCACAAGAAAAGTATCATCAACCAGCGTGAAGTCGGTGTGGACACCCGCAGTTTTCATGC
TGCACTGAAAAATACATTACGTCAGGCTCCGGACGTGATCCTGATCGGGGAAATCCGCGATCGTGAAACCATGGAACATG
CGCTGGCCTTTGCTGAAACCGGTCATTTGGCTATTTCAACGTTGCATGCTAACAATGCGAACCAGGCACTGGATCGTATT
ATCAACTTCTTCCCGGAAGAGCGGCACGCGCAGCTGATGAACGATCTGGGTAATAACCTGAAGGCGTTTGTATCACAACG
ACTGGTAAAAACAGTGGATGGCAAGCGTCATGCTGCGGTCGAGATTTTGTTAGGTACGGCAACCATTCAGGATATGATCC
GCCGTGGTGATTTCTCCAACATCAAAGAAATCATGGAGAAATCGGTCAACCTGGGTATGAAGACCTTTGACCAGTGTCTG
TTTGAGCTTTATTGCGAAGGCATTATTGATGAGGAAGAGGCATTGCGTAATGCAGACTCCCTCAATAACCTGAAGCTGAA
GATCAAATTACATGATGAAAATGGCGCTCTGGAAATCAAAGGGCCGGCCTTGAACTGGGAACTTGATCCTGTCAAACGTG
ATGAGCCGGGTTTCCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.704

99.736

0.536
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

47.297

97.625

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.114

88.127

0.38
  pilT Pseudomonas aeruginosa PAK

41.716

89.182

0.372
  pilT Neisseria gonorrhoeae MS11

40.634

91.557

0.372
  pilT Neisseria meningitidis 8013

40.634

91.557

0.372
  pilT Pseudomonas stutzeri DSM 10701

40.698

90.765

0.369
  pilT Acinetobacter baylyi ADP1

41.515

87.071

0.361

Multiple sequence alignment