Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ALT831_RS14785 Genome accession   NZ_OX359241
Coordinates   3353914..3355119 (+) Length   401 a.a.
NCBI ID   WP_049586077.1    Uniprot ID   -
Organism   Alteromonas macleodii isolate Alteromonas macleodii MIT1002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3348914..3360119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALT831_RS14760 (MIT1002_03005) ampE 3349767..3350669 (-) 903 WP_049586084.1 beta-lactamase regulator AmpE -
  ALT831_RS14765 (MIT1002_03006) ampD 3350712..3351251 (-) 540 WP_049586083.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ALT831_RS14770 (MIT1002_03007) - 3351435..3351953 (+) 519 WP_014950400.1 TIGR02281 family clan AA aspartic protease -
  ALT831_RS14775 (MIT1002_03008) nadC 3351986..3352840 (+) 855 WP_049586081.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ALT831_RS14780 (MIT1002_03009) - 3353419..3353844 (+) 426 WP_231514363.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ALT831_RS14785 (MIT1002_03010) pilC 3353914..3355119 (+) 1206 WP_049586077.1 type II secretion system F family protein Machinery gene
  ALT831_RS14790 (MIT1002_03011) pilD 3355172..3356065 (+) 894 WP_049586076.1 A24 family peptidase Machinery gene
  ALT831_RS14795 (MIT1002_03012) coaE 3356084..3356695 (+) 612 WP_049586074.1 dephospho-CoA kinase -
  ALT831_RS14800 (MIT1002_03013) zapD 3356939..3357691 (+) 753 WP_049586072.1 cell division protein ZapD -
  ALT831_RS14805 (MIT1002_03014) yacG 3357825..3358052 (+) 228 WP_049586071.1 DNA gyrase inhibitor YacG -
  ALT831_RS14810 (MIT1002_03015) - 3358272..3359144 (+) 873 WP_049586069.1 aspartoacylase -
  ALT831_RS14815 (MIT1002_03016) mutT 3359266..3359649 (-) 384 WP_049586068.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 401 a.a.        Molecular weight: 43876.60 Da        Isoelectric Point: 9.6768

>NTDB_id=1155765 ALT831_RS14785 WP_049586077.1 3353914..3355119(+) (pilC) [Alteromonas macleodii isolate Alteromonas macleodii MIT1002]
MKATIVFEWQGKDRQGLSRKGEISAASLSEAKNLLRRQGISANRVKKPSKSLFRREKKITPADISVISRQIATMLAAGVT
LIQSLEMISQGHANSSMRKLLGEITDEVKAGNPLSSALRKHPLYFDDLYCDLVYTGEQSGALETIYDRIATYKEKAEALK
SKIKKAMFYPIAVLVVAFIVTTILLIFVVPQFEEIFSSFGAELPAFTQFVLAISRFVQDYGIFIAIGIGGAGFMFMRTYK
RSQKLRDTVDRNILKIPVIGEILKKASIARFTRTLATTFAAGVPLIGALESAAGASGNAVYRDAILYMRKEVAGGMPMHV
AMRATQVFPDMVTQMIAIGEESGAVDEMLSKIATIYEAEVDDMVDGLTSLLEPMIMAVLGVVIGGLIVAMYLPIFQMGNV
V

Nucleotide


Download         Length: 1206 bp        

>NTDB_id=1155765 ALT831_RS14785 WP_049586077.1 3353914..3355119(+) (pilC) [Alteromonas macleodii isolate Alteromonas macleodii MIT1002]
ATGAAAGCCACGATAGTTTTCGAATGGCAAGGAAAGGACCGCCAAGGTCTATCTCGAAAGGGAGAAATATCGGCTGCATC
ACTCTCTGAAGCTAAGAATCTTTTACGTCGCCAAGGTATTTCAGCCAACAGAGTTAAAAAGCCATCTAAATCATTATTTA
GACGTGAAAAGAAAATCACACCCGCGGATATTTCTGTTATTTCACGTCAAATCGCCACCATGCTTGCCGCCGGTGTTACC
CTTATCCAATCGTTAGAAATGATTTCCCAAGGTCATGCCAATTCTTCAATGCGCAAACTTCTTGGCGAAATAACCGATGA
AGTAAAAGCGGGTAATCCATTGTCATCGGCGCTCAGAAAGCACCCCCTTTATTTTGACGATTTATATTGCGATTTGGTTT
ACACCGGTGAGCAGTCGGGCGCACTTGAAACCATTTATGACCGCATTGCGACCTACAAAGAAAAGGCCGAAGCACTAAAA
TCAAAAATCAAAAAAGCGATGTTTTACCCTATCGCGGTTTTGGTTGTTGCTTTTATTGTTACGACAATCCTTCTTATATT
TGTTGTACCGCAGTTTGAAGAAATTTTCAGCAGCTTTGGGGCAGAGCTCCCCGCCTTCACCCAGTTTGTATTGGCTATTT
CCCGATTCGTGCAAGACTACGGTATTTTTATCGCCATCGGTATTGGTGGTGCAGGCTTTATGTTTATGCGTACTTACAAG
CGAAGCCAAAAGCTCCGTGACACGGTGGACCGCAACATTTTAAAAATTCCCGTCATTGGCGAAATATTAAAAAAAGCCAG
CATTGCGCGCTTTACCCGAACCCTAGCCACGACCTTCGCAGCAGGTGTTCCGCTAATAGGTGCACTTGAATCTGCGGCGG
GAGCCTCGGGTAATGCGGTATATCGCGATGCCATATTGTATATGCGCAAAGAGGTGGCGGGCGGTATGCCTATGCATGTT
GCTATGCGCGCAACACAAGTCTTTCCTGACATGGTTACGCAAATGATTGCCATTGGTGAAGAGTCTGGAGCCGTCGATGA
AATGCTAAGTAAAATTGCTACTATTTACGAAGCTGAAGTTGACGATATGGTAGATGGCCTCACCAGCCTACTTGAACCTA
TGATCATGGCGGTACTTGGTGTGGTGATTGGTGGCCTTATTGTGGCTATGTATCTTCCTATATTCCAAATGGGTAACGTG
GTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(68-190)

Type II secretion system (T2SS), protein F (T2SSF)

(271-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.596

98.753

0.589
  pilC Acinetobacter baumannii D1279779

52.897

99.002

0.524
  pilC Acinetobacter baylyi ADP1

52.513

99.252

0.521
  pilC Legionella pneumophila strain ERS1305867

52.01

99.252

0.516
  pilC Vibrio cholerae strain A1552

46.465

98.753

0.459
  pilG Neisseria meningitidis 44/76-A

45.974

96.01

0.441
  pilG Neisseria gonorrhoeae MS11

45.714

96.01

0.439
  pilC Vibrio campbellii strain DS40M4

43.939

98.753

0.434
  pilC Thermus thermophilus HB27

37.905

100

0.379

Multiple sequence alignment