Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   QML74_RS05345 Genome accession   NZ_OX346404
Coordinates   1044923..1045393 (+) Length   156 a.a.
NCBI ID   WP_281957977.1    Uniprot ID   -
Organism   Enterococcus cecorum isolate CIRMBP-1281     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1006697..1059173 1044923..1045393 within 0


Gene organization within MGE regions


Location: 1006697..1059173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QML74_RS05160 (CIRMBP1281_01040) - 1007992..1008438 (+) 447 WP_114975413.1 hypothetical protein -
  QML74_RS05165 (CIRMBP1281_01041) - 1008439..1009134 (-) 696 WP_114975414.1 hypothetical protein -
  QML74_RS05170 (CIRMBP1281_01042) - 1009269..1009478 (+) 210 WP_047338730.1 helix-turn-helix transcriptional regulator -
  QML74_RS05175 (CIRMBP1281_01043) - 1009618..1011948 (+) 2331 WP_281957971.1 isopeptide-forming domain-containing fimbrial protein -
  QML74_RS05180 (CIRMBP1281_01044) - 1012007..1013014 (+) 1008 WP_281957972.1 isopeptide-forming domain-containing fimbrial protein -
  QML74_RS05185 (CIRMBP1281_01045) - 1013047..1013964 (+) 918 WP_281957973.1 hypothetical protein -
  QML74_RS05190 (CIRMBP1281_01046) - 1014078..1014335 (+) 258 WP_171332632.1 hypothetical protein -
  QML74_RS05195 (CIRMBP1281_01047) - 1014336..1014569 (+) 234 WP_047338943.1 TrbC/VirB2 family protein -
  QML74_RS05200 (CIRMBP1281_01048) - 1014625..1016856 (+) 2232 WP_281957974.1 hypothetical protein -
  QML74_RS05205 (CIRMBP1281_01049) - 1016843..1019542 (+) 2700 WP_281957975.1 type IV secretory system conjugative DNA transfer family protein -
  QML74_RS05210 (CIRMBP1281_01050) - 1019564..1022317 (+) 2754 WP_243180007.1 pLS20_p028 family conjugation system transmembrane protein -
  QML74_RS05215 (CIRMBP1281_01051) - 1022382..1022723 (+) 342 WP_047334877.1 DUF5592 family protein -
  QML74_RS05220 (CIRMBP1281_01052) - 1022725..1023324 (+) 600 WP_047334876.1 hypothetical protein -
  QML74_RS05225 (CIRMBP1281_01053) - 1023489..1025435 (+) 1947 WP_087216189.1 hypothetical protein -
  QML74_RS05230 - 1025537..1026130 (+) 594 Protein_992 lysozyme family protein -
  QML74_RS05235 - 1026194..1026544 (+) 351 Protein_993 C40 family peptidase -
  QML74_RS05240 (CIRMBP1281_01055) - 1026592..1027353 (+) 762 WP_281957976.1 hypothetical protein -
  QML74_RS05245 (CIRMBP1281_01056) - 1027366..1028475 (+) 1110 WP_243180009.1 hypothetical protein -
  QML74_RS05250 (CIRMBP1281_01057) - 1028744..1029190 (+) 447 WP_171378602.1 hypothetical protein -
  QML74_RS05255 (CIRMBP1281_01058) - 1029218..1029502 (+) 285 WP_243180010.1 hypothetical protein -
  QML74_RS05260 (CIRMBP1281_01059) - 1029495..1029716 (+) 222 WP_243180012.1 hypothetical protein -
  QML74_RS05265 (CIRMBP1281_01060) - 1029729..1030007 (+) 279 WP_047335105.1 hypothetical protein -
  QML74_RS05270 (CIRMBP1281_01061) - 1030029..1030232 (+) 204 WP_047335106.1 hypothetical protein -
  QML74_RS05275 (CIRMBP1281_01062) - 1030249..1030767 (+) 519 WP_243180015.1 hypothetical protein -
  QML74_RS05280 (CIRMBP1281_01063) radC 1031125..1031574 (+) 450 WP_047335108.1 DNA repair protein RadC -
  QML74_RS05285 (CIRMBP1281_01064) - 1032014..1032337 (+) 324 WP_168930126.1 hypothetical protein -
  QML74_RS05290 (CIRMBP1281_01065) - 1032321..1032677 (+) 357 WP_047338929.1 hypothetical protein -
  QML74_RS05295 (CIRMBP1281_01066) mobP2 1032696..1034591 (+) 1896 WP_243180017.1 MobP2 family relaxase -
  QML74_RS05300 (CIRMBP1281_01067) - 1034745..1036070 (+) 1326 WP_243180283.1 type III toxin-antitoxin system ToxN/AbiQ family toxin -
  QML74_RS05305 (CIRMBP1281_01068) - 1036231..1036551 (+) 321 WP_168930123.1 hypothetical protein -
  QML74_RS05310 (CIRMBP1281_01069) - 1036579..1037040 (+) 462 WP_171378613.1 hypothetical protein -
  QML74_RS05315 (CIRMBP1281_01070) - 1037040..1039052 (+) 2013 WP_243180281.1 LPD1 domain-containing protein -
  QML74_RS05320 (CIRMBP1281_01071) - 1039185..1040342 (+) 1158 WP_243180279.1 DUF3991 domain-containing protein -
  QML74_RS05325 (CIRMBP1281_01072) - 1040339..1041112 (+) 774 WP_052836427.1 thermonuclease family protein -
  QML74_RS05330 (CIRMBP1281_01073) - 1041121..1041846 (+) 726 WP_243180277.1 class A sortase -
  QML74_RS05335 (CIRMBP1281_01074) - 1041885..1042304 (+) 420 WP_243192884.1 hypothetical protein -
  QML74_RS05340 (CIRMBP1281_01075) - 1042301..1044463 (+) 2163 WP_171378620.1 type IA DNA topoisomerase -
  QML74_RS05345 (CIRMBP1281_01076) ssb 1044923..1045393 (+) 471 WP_281957977.1 single-stranded DNA-binding protein Machinery gene
  QML74_RS05350 (CIRMBP1281_01077) - 1045468..1045728 (+) 261 WP_047338919.1 hypothetical protein -
  QML74_RS05355 (CIRMBP1281_01078) - 1045831..1046124 (+) 294 WP_087216392.1 hypothetical protein -
  QML74_RS05360 (CIRMBP1281_01079) - 1046227..1046439 (+) 213 WP_047334073.1 hypothetical protein -
  QML74_RS05365 (CIRMBP1281_01080) - 1046454..1047272 (+) 819 WP_171332645.1 ParA family protein -
  QML74_RS05370 - 1047381..1047632 (+) 252 WP_047334075.1 hypothetical protein -
  QML74_RS05375 (CIRMBP1281_01081) - 1047662..1049047 (+) 1386 WP_171332646.1 ISLre2 family transposase -
  QML74_RS05380 (CIRMBP1281_01082) glyQ 1050203..1051111 (+) 909 WP_016251894.1 glycine--tRNA ligase subunit alpha -
  QML74_RS05385 (CIRMBP1281_01083) glyS 1051116..1053182 (+) 2067 WP_281957978.1 glycine--tRNA ligase subunit beta -
  QML74_RS05390 (CIRMBP1281_01084) - 1053423..1054103 (-) 681 WP_047242068.1 metal-dependent hydrolase -
  QML74_RS05395 (CIRMBP1281_01085) - 1054167..1055114 (-) 948 WP_047242067.1 AEC family transporter -
  QML74_RS05400 (CIRMBP1281_01086) yidC 1055213..1056175 (-) 963 WP_016251898.1 membrane protein insertase YidC -
  QML74_RS05405 (CIRMBP1281_01087) - 1056261..1056548 (-) 288 WP_016251899.1 acylphosphatase -
  QML74_RS05410 (CIRMBP1281_01088) - 1056651..1057418 (+) 768 WP_016251900.1 RNA methyltransferase -
  QML74_RS05415 (CIRMBP1281_01089) - 1057508..1058002 (+) 495 WP_047242066.1 hypothetical protein -
  QML74_RS05420 (CIRMBP1281_01090) - 1058131..1058823 (+) 693 WP_281957979.1 DUF5105 domain-containing protein -

Sequence


Protein


Download         Length: 156 a.a.        Molecular weight: 17620.47 Da        Isoelectric Point: 6.3633

>NTDB_id=1155034 QML74_RS05345 WP_281957977.1 1044923..1045393(+) (ssb) [Enterococcus cecorum isolate CIRMBP-1281]
MINNVTLVGRLTRDADLRYTASGVAVATFNLAVNRNYSNQNGERETDFINCVIWRKPAENLANYTRKGSLIGLTGRLQTR
NYENQQGQRVYVTEVIVENFQMLESREVTKNRKQAANQTNQASNPTNTTSQPTQPFMDYSSFGGQSVEISEDDLPF

Nucleotide


Download         Length: 471 bp        

>NTDB_id=1155034 QML74_RS05345 WP_281957977.1 1044923..1045393(+) (ssb) [Enterococcus cecorum isolate CIRMBP-1281]
ATGATTAACAACGTCACATTAGTAGGTAGATTAACAAGAGATGCAGACTTACGATATACTGCATCAGGAGTCGCAGTAGC
AACATTTAATTTAGCGGTTAATCGTAACTATAGCAATCAAAATGGCGAAAGAGAAACCGACTTTATCAATTGTGTGATTT
GGCGAAAACCTGCGGAAAATTTAGCAAATTATACAAGAAAAGGTAGCTTGATTGGTTTAACAGGTAGATTGCAAACAAGG
AATTATGAAAATCAACAAGGACAACGTGTGTATGTGACCGAAGTAATTGTAGAGAATTTTCAGATGTTAGAGTCCAGGGA
AGTCACTAAAAATCGCAAACAGGCAGCAAACCAAACTAATCAAGCATCTAATCCAACTAATACTACATCTCAACCAACAC
AACCATTTATGGATTATTCATCCTTTGGTGGCCAAAGTGTAGAAATTAGTGAAGATGATCTACCATTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

58.046

100

0.647

  ssbA Bacillus subtilis subsp. subtilis str. 168

50.581

100

0.558

  ssbB Bacillus subtilis subsp. subtilis str. 168

53.846

75

0.404

  ssbB/cilA Streptococcus mitis NCTC 12261

50.847

75.641

0.385

  ssbB/cilA Streptococcus pneumoniae TIGR4

50.847

75.641

0.385

  ssbB Streptococcus sobrinus strain NIDR 6715-7

43.066

87.821

0.378

  ssbB/cilA Streptococcus pneumoniae Rx1

50

75.641

0.378

  ssbB/cilA Streptococcus pneumoniae D39

50

75.641

0.378

  ssbB/cilA Streptococcus pneumoniae R6

50

75.641

0.378

  ssbB/cilA Streptococcus mitis SK321

50

75.641

0.378


Multiple sequence alignment