Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MIT1002_RS14830 Genome accession   NZ_OX335040
Coordinates   3429618..3430826 (+) Length   402 a.a.
NCBI ID   WP_156078731.1    Uniprot ID   -
Organism   Alteromonas macleodii isolate Alteromonas macleodii 83-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3424618..3435826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIT1002_RS14805 (ALT831_02995) ampE 3425466..3426368 (-) 903 WP_156078729.1 beta-lactamase regulator AmpE -
  MIT1002_RS14810 (ALT831_02996) ampD 3426411..3426950 (-) 540 WP_101565567.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MIT1002_RS14815 (ALT831_02997) - 3427134..3427652 (+) 519 WP_014950400.1 retropepsin-like aspartic protease family protein -
  MIT1002_RS14820 (ALT831_02998) nadC 3427685..3428539 (+) 855 WP_014977217.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MIT1002_RS14825 (ALT831_02999) - 3429109..3429546 (+) 438 WP_156078730.1 pilin -
  MIT1002_RS14830 (ALT831_03000) pilC 3429618..3430826 (+) 1209 WP_156078731.1 type II secretion system F family protein Machinery gene
  MIT1002_RS14835 (ALT831_03001) pilD 3430879..3431772 (+) 894 WP_156078732.1 prepilin peptidase Machinery gene
  MIT1002_RS14840 (ALT831_03002) coaE 3431791..3432402 (+) 612 WP_075176907.1 dephospho-CoA kinase -
  MIT1002_RS14845 (ALT831_03003) zapD 3432646..3433398 (+) 753 WP_039227984.1 cell division protein ZapD -
  MIT1002_RS14850 (ALT831_03004) yacG 3433532..3433759 (+) 228 WP_014950407.1 DNA gyrase inhibitor YacG -
  MIT1002_RS14855 (ALT831_03005) - 3433979..3434851 (+) 873 WP_039227982.1 aspartoacylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43706.43 Da        Isoelectric Point: 9.6771

>NTDB_id=1154693 MIT1002_RS14830 WP_156078731.1 3429618..3430826(+) (pilC) [Alteromonas macleodii isolate Alteromonas macleodii 83-1]
MAKALITFVWQGKDRRGQSRKGEISAASLSEAKNLLRRQGISANKVKKLSKPLFGGSQKITAADISVISRQIATMLAAGV
TLIQSLEMISQGHANSSMRKLLGEITDEVKAGNPLSSALRKHPLYFDDLYCDLVYTGEQSGALETIYDRIATYKEKAEAL
KSKIKKAMFYPIAVLVVAFIVTTILLIFVVPQFEEIFSSFGAELPAFTQFVLAISRFVQDYGIFIAIGIGGAGFMFMRTY
KRSQKLRDTVDRNILKIPVIGEILKKASIARFTRTLATTFAAGVPLIGALESAAGASGNAVYRDAILYMRKEVAGGMPMH
VAMRATQVFPDMVTQMIAIGEESGAVDEMLSKIATIYEAEVDDMVDGLTSLLEPMIMAVLGVVIGGLIVAMYLPIFQMGN
VV

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=1154693 MIT1002_RS14830 WP_156078731.1 3429618..3430826(+) (pilC) [Alteromonas macleodii isolate Alteromonas macleodii 83-1]
TTGGCAAAAGCACTTATAACTTTTGTGTGGCAAGGGAAAGACCGCCGAGGTCAGTCAAGGAAAGGTGAAATATCCGCAGC
CTCTTTATCAGAAGCAAAAAACCTACTTCGACGCCAAGGAATCTCAGCAAACAAAGTTAAAAAGCTATCTAAGCCTCTTT
TTGGCGGAAGCCAGAAAATTACTGCTGCAGACATCTCTGTCATTTCTCGTCAAATCGCCACCATGCTCGCCGCCGGTGTT
ACCCTTATTCAATCGTTAGAAATGATTTCCCAAGGTCACGCCAATTCTTCAATGCGCAAACTTCTTGGCGAAATAACCGA
TGAGGTAAAAGCGGGTAATCCACTTTCATCAGCGCTTAGAAAGCACCCCCTTTATTTTGACGATTTATATTGCGACTTGG
TTTATACCGGTGAGCAGTCGGGCGCACTTGAAACCATTTATGACCGCATTGCGACTTATAAAGAGAAGGCCGAAGCACTA
AAATCAAAAATCAAAAAAGCGATGTTTTATCCTATCGCGGTTTTGGTTGTTGCTTTTATTGTTACGACCATCCTTCTTAT
ATTTGTTGTGCCGCAGTTTGAAGAGATTTTCAGCAGCTTTGGCGCAGAACTCCCTGCCTTTACCCAGTTCGTATTGGCTA
TTTCACGGTTCGTCCAAGACTACGGTATTTTTATCGCCATAGGTATTGGTGGTGCTGGGTTTATGTTTATGCGCACCTAT
AAGCGAAGCCAGAAGCTACGTGACACGGTAGACCGCAACATTTTAAAAATTCCCGTTATCGGCGAGATACTAAAAAAAGC
CAGTATTGCGCGCTTCACCCGAACCCTAGCCACGACCTTCGCAGCAGGTGTGCCGCTTATTGGCGCACTAGAATCCGCCG
CCGGTGCATCGGGCAACGCAGTATACCGCGATGCCATTTTATACATGCGTAAAGAAGTGGCAGGCGGTATGCCTATGCAC
GTTGCAATGCGCGCTACACAAGTATTCCCTGACATGGTGACGCAGATGATTGCCATTGGTGAGGAGTCGGGCGCGGTTGA
TGAAATGCTCAGCAAAATTGCCACCATATATGAAGCTGAAGTCGATGATATGGTAGACGGTTTAACTAGCCTACTTGAAC
CTATGATCATGGCCGTACTTGGCGTGGTAATCGGCGGCTTGATTGTGGCTATGTACCTCCCTATATTCCAGATGGGTAAC
GTGGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.734

98.259

0.577
  pilC Acinetobacter baumannii D1279779

52.605

100

0.527
  pilC Legionella pneumophila strain ERS1305867

52.632

99.254

0.522
  pilC Acinetobacter baylyi ADP1

52.141

98.756

0.515
  pilC Vibrio cholerae strain A1552

46.115

99.254

0.458
  pilG Neisseria meningitidis 44/76-A

44.307

100

0.445
  pilG Neisseria gonorrhoeae MS11

44.059

100

0.443
  pilC Vibrio campbellii strain DS40M4

43.921

100

0.44
  pilC Thermus thermophilus HB27

36.842

99.254

0.366

Multiple sequence alignment