Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F544_RS04870 Genome accession   NZ_CP006956
Coordinates   1025841..1027238 (-) Length   465 a.a.
NCBI ID   WP_025289239.1    Uniprot ID   W0R6Z9
Organism   Bibersteinia trehalosi USDA-ARS-USMARC-190     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1020841..1032238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F544_RS04840 (F544_9670) - 1021201..1022385 (+) 1185 WP_025267131.1 FAD-dependent monooxygenase -
  F544_RS04845 (F544_9680) - 1022520..1023074 (+) 555 WP_025267130.1 hypothetical protein -
  F544_RS04850 (F544_9690) yacG 1023134..1023340 (-) 207 WP_015432346.1 DNA gyrase inhibitor YacG -
  F544_RS04855 (F544_9700) coaE 1023340..1023960 (-) 621 WP_025289236.1 dephospho-CoA kinase -
  F544_RS04860 (F544_9710) - 1023990..1024655 (-) 666 WP_025289237.1 prepilin peptidase -
  F544_RS04865 (F544_9720) pilC 1024652..1025848 (-) 1197 WP_025289238.1 type II secretion system F family protein Machinery gene
  F544_RS04870 (F544_9730) pilB 1025841..1027238 (-) 1398 WP_025289239.1 GspE/PulE family protein Machinery gene
  F544_RS04875 (F544_9740) pilA 1027296..1027742 (-) 447 WP_025267127.1 prepilin peptidase-dependent pilin Machinery gene
  F544_RS04880 (F544_9750) radA 1027917..1029296 (+) 1380 WP_025289240.1 DNA repair protein RadA Machinery gene
  F544_RS04885 (F544_9760) - 1029388..1030932 (-) 1545 WP_025289241.1 Na+/H+ antiporter NhaC family protein -
  F544_RS04890 (F544_9780) - 1031437..1031838 (+) 402 WP_015432355.1 H-NS family nucleoid-associated regulatory protein -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 53355.15 Da        Isoelectric Point: 5.6134

>NTDB_id=115244 F544_RS04870 WP_025289239.1 1025841..1027238(-) (pilB) [Bibersteinia trehalosi USDA-ARS-USMARC-190]
MSKYTICALNSSTQFEISERQWQKNCDEKQLLRRYIAMPLQENEQKIWLALDDENNLNACEIFTFLTHKQVEPVLVESEE
LKFLLNQLSPENEVAIDFDEFQTLHIDEQTFSDGLDLNDPIIRTLDNLFKFCLQKNASDIHIEPHKQHLMIRLRIDGVLQ
LYKTLPTHFSSRLLSRLKLLAKLDISENRLPQDGQFSFTTELNEILDFRVSTLPVQYGEKMVLRLQKNKPTHLDFLSLGF
TQAQQEKLLNALKQPQGIILVTGPTGSGKSITLYSALNYLNQSDKHILTAEDPIEIEIEGLIQTQVNHAIQLDFSRLLRT
FLRQDPDIIMLGEIRDEESAEIALRAAQTGHLVLSTLHTNDAPSAIERLNQLGIKDYEIKNSLLLVIAQRLVRLICPKCK
GKGCQECYQGYKGRIGVFQLFSRVAEQFEKSTACLDYPTLQESAKHKLAENLTDLHEIERVFGHV

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=115244 F544_RS04870 WP_025289239.1 1025841..1027238(-) (pilB) [Bibersteinia trehalosi USDA-ARS-USMARC-190]
ATGTCTAAATACACCATCTGCGCACTCAACTCATCAACCCAGTTTGAAATCAGTGAACGACAATGGCAGAAAAACTGCGA
TGAAAAGCAACTACTACGCCGTTATATTGCTATGCCTCTGCAGGAAAATGAACAGAAAATCTGGCTGGCGCTTGATGATG
AAAATAATCTAAATGCTTGTGAAATTTTTACTTTTCTCACGCATAAACAGGTTGAACCTGTATTAGTTGAAAGCGAAGAA
TTGAAATTTTTACTTAATCAGTTATCCCCTGAAAATGAGGTAGCAATAGATTTTGATGAATTTCAAACTCTACATATCGA
TGAGCAAACATTCTCTGACGGACTTGATTTGAATGATCCGATTATTCGCACTCTAGATAATCTATTTAAATTCTGTTTAC
AGAAAAATGCTTCTGATATTCACATTGAGCCTCATAAACAACATTTAATGATTCGTCTGCGCATTGATGGCGTACTCCAA
CTTTATAAAACCTTACCCACCCATTTTTCAAGTCGTTTACTTTCTCGTCTGAAACTATTAGCCAAGCTAGATATCAGTGA
AAATCGTTTACCACAAGATGGACAGTTTAGCTTTACTACCGAACTGAATGAAATCTTAGATTTTCGTGTTTCTACCCTAC
CTGTGCAATATGGTGAAAAAATGGTTCTGCGCTTACAAAAAAATAAACCTACTCACTTGGATTTTCTCTCGCTCGGTTTT
ACCCAAGCGCAACAAGAGAAACTGCTCAATGCTTTAAAACAACCTCAAGGAATAATTCTAGTAACAGGCCCAACTGGAAG
TGGTAAAAGTATTACGCTCTATAGTGCATTGAATTATTTAAACCAAAGCGATAAGCATATTCTTACTGCTGAAGATCCTA
TTGAAATTGAGATTGAAGGCTTAATTCAAACGCAAGTAAACCACGCTATTCAACTTGATTTTAGCCGGCTATTACGTACA
TTTCTCCGCCAAGATCCAGATATTATTATGCTGGGAGAAATTCGAGATGAAGAGAGCGCCGAAATTGCATTACGAGCAGC
ACAAACAGGCCATCTAGTGCTTTCTACTTTACATACAAATGATGCACCCTCAGCGATTGAACGCCTAAATCAACTCGGTA
TTAAAGACTATGAAATAAAAAATTCACTGTTATTAGTGATTGCTCAACGTTTGGTTCGCCTTATTTGCCCTAAATGCAAG
GGCAAAGGTTGCCAAGAATGTTATCAAGGCTACAAAGGACGAATAGGCGTATTTCAACTTTTCTCTCGTGTTGCAGAACA
GTTTGAAAAATCAACCGCTTGCTTAGACTATCCCACATTACAGGAAAGCGCAAAGCACAAATTAGCTGAAAATCTAACCG
ACTTGCATGAAATTGAACGGGTATTTGGCCATGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(31-91)

Type II/IV secretion system protein (T2SSE)

(133-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W0R6Z9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

70.69

99.785

0.705
  pilB Haemophilus influenzae 86-028NP

57.363

97.849

0.561
  pilB Haemophilus influenzae Rd KW20

57.143

97.849

0.559
  pilB Vibrio cholerae strain A1552

40.664

100

0.422
  pilB Vibrio campbellii strain DS40M4

39.709

100

0.411
  pilB Vibrio parahaemolyticus RIMD 2210633

37.575

100

0.406
  pilB Legionella pneumophila strain ERS1305867

37.398

100

0.396
  pilB Acinetobacter baumannii D1279779

37.195

100

0.394
  pilB Acinetobacter baylyi ADP1

37.295

100

0.391
  pilF Thermus thermophilus HB27

35.865

100

0.366

Multiple sequence alignment