Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   F544_RS04880 Genome accession   NZ_CP006956
Coordinates   1027917..1029296 (+) Length   459 a.a.
NCBI ID   WP_025289240.1    Uniprot ID   W0R509
Organism   Bibersteinia trehalosi USDA-ARS-USMARC-190     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1022917..1034296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F544_RS04850 (F544_9690) yacG 1023134..1023340 (-) 207 WP_015432346.1 DNA gyrase inhibitor YacG -
  F544_RS04855 (F544_9700) coaE 1023340..1023960 (-) 621 WP_025289236.1 dephospho-CoA kinase -
  F544_RS04860 (F544_9710) - 1023990..1024655 (-) 666 WP_025289237.1 prepilin peptidase -
  F544_RS04865 (F544_9720) pilC 1024652..1025848 (-) 1197 WP_025289238.1 type II secretion system F family protein Machinery gene
  F544_RS04870 (F544_9730) pilB 1025841..1027238 (-) 1398 WP_025289239.1 GspE/PulE family protein Machinery gene
  F544_RS04875 (F544_9740) pilA 1027296..1027742 (-) 447 WP_025267127.1 prepilin peptidase-dependent pilin Machinery gene
  F544_RS04880 (F544_9750) radA 1027917..1029296 (+) 1380 WP_025289240.1 DNA repair protein RadA Machinery gene
  F544_RS04885 (F544_9760) - 1029388..1030932 (-) 1545 WP_025289241.1 Na+/H+ antiporter NhaC family protein -
  F544_RS04890 (F544_9780) - 1031437..1031838 (+) 402 WP_015432355.1 H-NS family nucleoid-associated regulatory protein -
  F544_RS04895 (F544_9790) rnhA 1032181..1032639 (+) 459 WP_025289242.1 ribonuclease HI -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49546.24 Da        Isoelectric Point: 8.3945

>NTDB_id=115246 F544_RS04880 WP_025289240.1 1027917..1029296(+) (radA) [Bibersteinia trehalosi USDA-ARS-USMARC-190]
MAKAPKTAYVCSDCGEEYSKWMGQCRACLAWNTISEVRLISSKQETKGTRFSGYAGQTSGKVQTLSEISLQEVPRFSSGF
NELDRVLGGGIVPGSAILIGGHPGAGKSTLLLQVMCGLSSRLPTLYVTGEESLQQVAMRANRLGLPTEHLKMLSETSVEH
ICNLADQEKPKLMVIDSIQVMHLADIQSSPGSVAQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHAIDASL
LLEGEADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVKNPSAIFLSRSEEQTSGSSVMVLWEGTRPLLVEIQALVDHS
MLANPRRVAVGLEQNRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALLLALLSSFRNRPLPHDLVVFGEVG
LAGEIRPVPSGQERISEAAKHGFKRAIVPAGNIPKKSIKGMEVLGVKKLSDALDIVANL

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=115246 F544_RS04880 WP_025289240.1 1027917..1029296(+) (radA) [Bibersteinia trehalosi USDA-ARS-USMARC-190]
ATGGCAAAAGCACCGAAAACCGCTTATGTATGTAGTGATTGTGGCGAAGAGTATTCAAAATGGATGGGGCAATGTCGTGC
TTGTTTAGCATGGAATACGATTAGTGAAGTACGGTTAATTTCGTCAAAACAAGAAACCAAAGGTACTCGCTTTAGTGGCT
ACGCGGGGCAAACTTCTGGTAAGGTACAAACGCTTTCAGAGATCAGCTTGCAGGAAGTACCAAGGTTTAGCAGTGGCTTT
AATGAACTAGATCGAGTATTAGGCGGAGGGATTGTACCTGGAAGTGCAATTTTAATTGGCGGGCATCCCGGGGCAGGAAA
AAGCACTTTATTACTCCAAGTTATGTGTGGGTTGTCATCAAGATTGCCAACACTCTATGTAACAGGTGAAGAATCATTAC
AACAGGTTGCCATGCGAGCAAATCGTTTGGGGTTACCAACCGAGCATTTGAAGATGCTTTCAGAAACCTCTGTTGAGCAT
ATTTGTAATCTTGCAGATCAGGAAAAACCCAAATTGATGGTTATTGATTCTATTCAGGTCATGCATCTTGCTGATATTCA
ATCTTCTCCAGGGAGTGTTGCACAGGTGCGAGAATGTGCTTCTTTTTTAACTCGTTATGCGAAAACCCGTCAAGTTGCGA
TTATTATGGTTGGACATGTGACGAAAGATGGTACATTAGCAGGTCCAAAAGTGTTAGAACATGCGATTGATGCTTCATTA
TTGTTAGAAGGGGAGGCAGATAGTCGCTTTAGAACCTTGCGTAGTCATAAGAACCGTTTTGGTGCAGTGAATGAACTCGG
TGTGTTTGCTATGACTGAGCAAGGTTTACGTGAAGTGAAAAATCCATCAGCGATTTTCTTAAGTCGAAGTGAAGAACAAA
CTTCTGGCAGTTCAGTTATGGTGCTTTGGGAAGGAACACGTCCATTATTAGTGGAAATTCAGGCATTAGTTGATCACTCT
ATGCTTGCCAATCCTCGCCGAGTTGCAGTGGGATTAGAGCAGAACCGTTTATCTCTATTACTAGCTGTTTTGCATCGTCA
TGGTGGCTTACAAATGTCTGATCAAGATGTGTTTGTCAATGTCGTTGGCGGAGTAAAAGTGACTGAAACTAGTGCAGATT
TAGCCCTGCTGTTAGCCTTGCTTTCTAGTTTTCGTAACCGACCATTGCCTCATGATCTGGTGGTTTTTGGTGAAGTAGGG
CTAGCAGGTGAAATCCGCCCAGTACCTAGTGGACAAGAGCGTATTAGTGAAGCCGCAAAACATGGATTTAAGCGCGCGAT
TGTCCCTGCTGGAAACATACCAAAGAAATCAATTAAGGGGATGGAAGTGCTTGGTGTGAAAAAGCTAAGTGATGCGTTGG
ATATTGTTGCGAATCTTTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(80-224)

Subunit ChlI of Mg-chelatase (ChlI)

(347-432)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W0R509

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.465

99.346

0.481
  radA Streptococcus mitis NCTC 12261

43.913

100

0.44
  radA Streptococcus mitis SK321

43.506

100

0.438
  radA Streptococcus pneumoniae TIGR4

43.545

99.564

0.434
  radA Streptococcus pneumoniae D39

43.545

99.564

0.434
  radA Streptococcus pneumoniae Rx1

43.545

99.564

0.434
  radA Streptococcus pneumoniae R6

43.545

99.564

0.434

Multiple sequence alignment