Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LSO74_RS04170 Genome accession   NZ_OV040719
Coordinates   788773..790167 (+) Length   464 a.a.
NCBI ID   WP_005656265.1    Uniprot ID   A0A0H3PF78
Organism   Haemophilus influenzae strain 3655 isolate 3655     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 783773..795167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSO74_RS04145 (KRLU3655_LOCUS784) rsmE 783793..784530 (-) 738 WP_005656289.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LSO74_RS04150 (KRLU3655_LOCUS785) lnt 784580..786109 (-) 1530 WP_079997635.1 apolipoprotein N-acyltransferase -
  LSO74_RS04155 (KRLU3655_LOCUS786) corC 786132..787031 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  LSO74_RS04160 (KRLU3655_LOCUS787) ampD 787652..788212 (-) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LSO74_RS04165 (KRLU3655_LOCUS788) pilA 788327..788776 (+) 450 WP_005656280.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LSO74_RS04170 (KRLU3655_LOCUS789) pilB 788773..790167 (+) 1395 WP_005656265.1 GspE/PulE family protein Machinery gene
  LSO74_RS04175 (KRLU3655_LOCUS790) pilC 790164..791384 (+) 1221 WP_005656262.1 type II secretion system F family protein Machinery gene
  LSO74_RS04180 (KRLU3655_LOCUS791) pilD 791381..792073 (+) 693 WP_005656260.1 prepilin peptidase Machinery gene
  LSO74_RS04185 (KRLU3655_LOCUS792) rho 792127..793389 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  LSO74_RS04190 (KRLU3655_LOCUS793) metJ 793637..793954 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  LSO74_RS04195 (KRLU3655_LOCUS794) cueR 793968..794354 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  LSO74_RS04200 (KRLU3655_LOCUS795) - 794431..794637 (+) 207 WP_005689491.1 heavy-metal-associated domain-containing protein -
  LSO74_RS04205 (KRLU3655_LOCUS796) - 794711..794917 (+) 207 WP_005660027.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52766.20 Da        Isoelectric Point: 6.1416

>NTDB_id=1151775 LSO74_RS04170 WP_005656265.1 788773..790167(+) (pilB) [Haemophilus influenzae strain 3655 isolate 3655]
MTSYALLHTQRVTAKNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSKC
GGNLANSCDCHQGYRGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVSQGITDGKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1151775 LSO74_RS04170 WP_005656265.1 788773..790167(+) (pilB) [Haemophilus influenzae strain 3655 isolate 3655]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTAAAAATGGCGAAGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTACGTTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTCTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAGCTTACTCCGCGTCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGTCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACGTGCGCTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATC
CCATTGAAATTGAGCTGGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGTCAAGATCCCGATATTATTATGCTGGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAGACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCGATATCTGCTATTTCTCGATTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAGCGTCTTGTACGAAAGCTTTGTTCAAAGTGC
GGTGGAAATTTAGCAAATTCTTGTGATTGCCATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTCCTACATTGGCA
GCAGAATGGTTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAGCCAAGGTATAACAGATGGAA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3PF78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

98.272

99.784

0.981
  pilB Haemophilus influenzae 86-028NP

96.983

100

0.97
  pilB Glaesserella parasuis strain SC1401

57.522

97.414

0.56
  pilB Vibrio cholerae strain A1552

39.848

100

0.453
  pilB Vibrio campbellii strain DS40M4

40.9

100

0.431
  pilB Legionella pneumophila strain ERS1305867

39.959

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.42
  pilB Acinetobacter baylyi ADP1

39.113

100

0.418
  pilB Acinetobacter baumannii D1279779

39.709

100

0.412
  pilF Neisseria gonorrhoeae MS11

37.395

100

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Thermus thermophilus HB27

35.924

100

0.369

Multiple sequence alignment