Detailed information NTG1151777

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: LSO74_RS04180
• Genome accession: NZ_OV040719
• Coordinates: 791381..792073 (+)
• Length: 230 a.a.
• NCBI ID: WP_005656260.1
• Uniprot ID: A0A0H3PEJ7
• Organism: Haemophilus influenzae strain 3655 isolate 3655
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 786381..797073

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSO74_RS04160 (KRLU3655_LOCUS787)	ampD	787652..788212 (-)	561	WP_005648974.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
LSO74_RS04165 (KRLU3655_LOCUS788)	pilA	788327..788776 (+)	450	WP_005656280.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
LSO74_RS04170 (KRLU3655_LOCUS789)	pilB	788773..790167 (+)	1395	WP_005656265.1	GspE/PulE family protein	Machinery gene
LSO74_RS04175 (KRLU3655_LOCUS790)	pilC	790164..791384 (+)	1221	WP_005656262.1	type II secretion system F family protein	Machinery gene
LSO74_RS04180 (KRLU3655_LOCUS791)	pilD	791381..792073 (+)	693	WP_005656260.1	prepilin peptidase	Machinery gene
LSO74_RS04185 (KRLU3655_LOCUS792)	rho	792127..793389 (-)	1263	WP_005648966.1	transcription termination factor Rho	-
LSO74_RS04190 (KRLU3655_LOCUS793)	metJ	793637..793954 (+)	318	WP_005631186.1	met regulon transcriptional regulator MetJ	-
LSO74_RS04195 (KRLU3655_LOCUS794)	cueR	793968..794354 (-)	387	WP_005648963.1	Cu(I)-responsive transcriptional regulator	-
LSO74_RS04200 (KRLU3655_LOCUS795)	-	794431..794637 (+)	207	WP_005689491.1	heavy-metal-associated domain-containing protein	-
LSO74_RS04205 (KRLU3655_LOCUS796)	-	794711..794917 (+)	207	WP_005660027.1	heavy-metal-associated domain-containing protein	-
LSO74_RS04210 (KRLU3655_LOCUS797)	-	794991..795197 (+)	207	WP_005656257.1	heavy-metal-associated domain-containing protein	-
LSO74_RS04215 (KRLU3655_LOCUS798)	-	795271..795477 (+)	207	WP_005656255.1	heavy-metal-associated domain-containing protein	-
LSO74_RS04220 (KRLU3655_LOCUS799)	-	795551..795757 (+)	207	WP_005656254.1	heavy-metal-associated domain-containing protein	-

Sequence

Protein

• Length: 230 a.a.
• Molecular weight: 26775.84 Da
• Isoelectric Point: 9.3065

>NTDB_id=1151777 LSO74_RS04180 WP_005656260.1 791381..792073(+) (pilD) [Haemophilus influenzae strain 3655 isolate 3655]
MIYFTMFLLGGILGIALWFYLSGFITRLQQDIYATYVELFPQNRSPFQPHFVSIQQKKCGHILRYFLSVGVGFIFLQIAF
KDSIFTVWIGLTLIILWTISYLDWHYQLISTTPCLWLLTLGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWIAKCYYR
KEAFGRGDYWLAMALGSFIRLETLPHFLLLASVLGICFSLIHKKKKEFIPFAPFMNLSAVIIYFVKYYGY

Nucleotide

• Length: 693 bp

>NTDB_id=1151777 LSO74_RS04180 WP_005656260.1 791381..792073(+) (pilD) [Haemophilus influenzae strain 3655 isolate 3655]
ATGATTTACTTCACAATGTTTTTATTAGGCGGCATCTTAGGGATCGCATTGTGGTTCTATCTATCTGGTTTTATTACGCG
TTTACAGCAAGATATTTATGCGACTTACGTTGAATTATTTCCACAAAACCGTTCTCCATTTCAACCGCACTTTGTCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTAAGTGTTGGAGTAGGATTTATATTTTTACAAATTGCCTTC
AAAGATTCTATTTTTACTGTATGGATCGGACTCACACTTATTATTCTTTGGACAATTAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACGACTCCCTGTTTATGGTTACTTACTCTAGGATTATTTGGAGCAGACAATAACTTTTCATTGCTAACGT
TATCTGAAAGCATAAAAAGTGCGGCTAGTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATGTTATTACAGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCGTTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTGCTTGGAATATGTTTTTCGCTTATTCATAAAAAGAAAAAAGAATTTATACCTTTTGCCCCTT
TTATGAACTTATCAGCTGTCATCATTTATTTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (92-199)

3D structure

• AlphaFold DB: A0A0H3PEJ7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Haemophilus influenzae 86-028NP	96.087	100	0.961
pilD	Haemophilus influenzae Rd KW20	94.783	100	0.948