Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LSO75_RS03655 Genome accession   NZ_OV040584
Coordinates   712526..713920 (+) Length   464 a.a.
NCBI ID   WP_044364868.1    Uniprot ID   -
Organism   Haemophilus influenzae isolate KR271     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 707526..718920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LSO75_RS03630 (KRLU271_LOCUS685) rsmE 707540..708277 (-) 738 WP_044364858.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LSO75_RS03635 (KRLU271_LOCUS686) lnt 708327..709856 (-) 1530 WP_080452425.1 apolipoprotein N-acyltransferase -
  LSO75_RS03640 (KRLU271_LOCUS687) corC 709879..710778 (-) 900 WP_044364861.1 CNNM family magnesium/cobalt transport protein CorC -
  LSO75_RS03645 (KRLU271_LOCUS688) ampD 711411..711965 (-) 555 WP_044364862.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LSO75_RS03650 (KRLU271_LOCUS689) pilA 712080..712529 (+) 450 WP_044364865.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LSO75_RS03655 (KRLU271_LOCUS690) pilB 712526..713920 (+) 1395 WP_044364868.1 GspE/PulE family protein Machinery gene
  LSO75_RS03660 (KRLU271_LOCUS691) pilC 713917..715137 (+) 1221 WP_011271971.1 type II secretion system F family protein Machinery gene
  LSO75_RS03665 (KRLU271_LOCUS692) pilD 715134..715826 (+) 693 WP_038440789.1 prepilin peptidase Machinery gene
  LSO75_RS03670 (KRLU271_LOCUS693) rho 715880..717142 (-) 1263 WP_005629550.1 transcription termination factor Rho -
  LSO75_RS03675 (KRLU271_LOCUS694) metJ 717390..717707 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  LSO75_RS03680 (KRLU271_LOCUS695) cueR 717721..718107 (-) 387 WP_005663006.1 Cu(I)-responsive transcriptional regulator -
  LSO75_RS03685 (KRLU271_LOCUS696) - 718184..718390 (+) 207 WP_012054944.1 heavy-metal-associated domain-containing protein -
  LSO75_RS03690 (KRLU271_LOCUS697) - 718476..718682 (+) 207 WP_005648955.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52984.41 Da        Isoelectric Point: 5.6445

>NTDB_id=1151740 LSO75_RS03655 WP_044364868.1 712526..713920(+) (pilB) [Haemophilus influenzae isolate KR271]
MTSYALLHTQRVIAQNGEIFTISPDLWERNRQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPCQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRSLSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1151740 LSO75_RS03655 WP_044364868.1 712526..713920(+) (pilB) [Haemophilus influenzae isolate KR271]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAATTGCTCAAAATGGCGAGATCTTTACGATCTCGCCAGATTTATG
GGAACGCAATCGGCAGCAACAATCCTTGCTTTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAGCAACTTACTCCGTGCCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCTCACTTAGCCAGCCACAAGGATTAATTTTAGTAACCGGCCCCACAGG
AAGTGGGAAAAGTATCTCGCTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAAGATC
CCATTGAAATTGAACTTGATGGTATTATTCAAAGCCAAATTAATCCGCAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAAAGTGCAAGGATTGCACTACGTGC
CGCTCAAACGGGACATTTGGTGCTTTCAACTTTACATACCAATGATGCAATATCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAACAACATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTGGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(25-95)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

99.138

100

0.991
  pilB Haemophilus influenzae Rd KW20

96.328

99.784

0.961
  pilB Glaesserella parasuis strain SC1401

57.826

99.138

0.573
  pilB Vibrio cholerae strain A1552

39.469

100

0.448
  pilB Vibrio campbellii strain DS40M4

40.082

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.059

100

0.412
  pilB Acinetobacter baylyi ADP1

38.105

100

0.407
  pilB Acinetobacter baumannii D1279779

38.557

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.377
  pilF Neisseria gonorrhoeae MS11

36.555

100

0.375
  pilF Thermus thermophilus HB27

36.134

100

0.371

Multiple sequence alignment