Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MKZ32_RS01240 Genome accession   NZ_OU912926
Coordinates   273104..274321 (-) Length   405 a.a.
NCBI ID   WP_239795601.1    Uniprot ID   -
Organism   Candidatus Nitrotoga arctica strain 6680 isolate 6680     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 268104..279321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKZ32_RS01210 (NTG6680_0246) - 268443..269141 (+) 699 WP_239795597.1 SDR family oxidoreductase -
  MKZ32_RS01215 (NTG6680_0247) - 269157..270170 (+) 1014 WP_275584236.1 Nudix family hydrolase -
  MKZ32_RS01220 yacG 270158..270346 (-) 189 WP_239798071.1 DNA gyrase inhibitor YacG -
  MKZ32_RS01225 (NTG6680_0248) zapD 270448..271203 (-) 756 WP_239795598.1 cell division protein ZapD -
  MKZ32_RS01230 (NTG6680_0249) coaE 271270..271896 (-) 627 WP_239795599.1 dephospho-CoA kinase -
  MKZ32_RS01235 (NTG6680_0250) pilD 271893..272765 (-) 873 WP_239795600.1 prepilin peptidase Machinery gene
  MKZ32_RS01240 (NTG6680_0251) pilC 273104..274321 (-) 1218 WP_239795601.1 type II secretion system F family protein Machinery gene
  MKZ32_RS01245 (NTG6680_0252) - 274908..275417 (+) 510 WP_338140774.1 alpha/beta hydrolase -
  MKZ32_RS15335 (NTG6680_0253) - 275550..275684 (+) 135 WP_275584237.1 hypothetical protein -
  MKZ32_RS01250 (NTG6680_0254) - 275681..275908 (+) 228 WP_239795603.1 acyl carrier protein -
  MKZ32_RS01255 (NTG6680_0255) - 275910..277136 (+) 1227 WP_239795604.1 beta-ketoacyl-[acyl-carrier-protein] synthase family protein -
  MKZ32_RS01260 (NTG6680_0257) - 277965..278912 (+) 948 WP_239795605.1 PEP-CTERM sorting domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44214.25 Da        Isoelectric Point: 10.0264

>NTDB_id=1151576 MKZ32_RS01240 WP_239795601.1 273104..274321(-) (pilC) [Candidatus Nitrotoga arctica strain 6680 isolate 6680]
MAAEKPKKKEIQFRWEGKDKFGKPVKGEVRAVGDAVAATQLRRLGINVTSIKKSHSSGGKKITQKDLTLFTRQLAVMMKS
GVPLLQSFDIVGKGHHNPSVSRLLNDIKTDVETGSSLEQAFRKFPLYFDDLFCNLIGAGEHAGILDSLLERLATYQEKTL
AIKSKIKGALMYPVSIIAIAFIITAVIMIFVIPAFKQLFSSFGADLPAPTLIMMSISDFFVAYWWAIFSIIGGGLYSFFY
FWKRNKKMQDVIDRIMLKLPIFGNVIRKASIARWTRTLATMFAAGVPLVEALNSVAGAAGNVVYFDATKIIQREVSQGGT
LTAAMQNVGVFPSMVLQMVAIGEEAGSLDGMLNKVADFFEAEVDDAVAALASLMEPIIMVVLGTLIGGMVIAMYLPIFKM
GQAVG

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1151576 MKZ32_RS01240 WP_239795601.1 273104..274321(-) (pilC) [Candidatus Nitrotoga arctica strain 6680 isolate 6680]
ATGGCAGCAGAAAAACCGAAAAAAAAAGAAATTCAATTTCGATGGGAAGGTAAAGATAAATTCGGCAAGCCGGTAAAAGG
CGAAGTACGGGCTGTCGGCGATGCCGTTGCTGCCACTCAGCTGAGACGTCTAGGCATTAATGTTACCAGTATCAAAAAAT
CCCACAGTAGCGGAGGAAAAAAGATCACCCAGAAAGATCTCACCCTGTTTACGCGCCAGCTAGCGGTCATGATGAAATCC
GGCGTACCGCTGCTACAATCATTCGACATTGTAGGCAAGGGACACCACAACCCTTCTGTTTCCCGCCTGCTGAACGACAT
TAAAACCGACGTAGAAACTGGTAGCAGCCTGGAACAGGCATTCCGGAAATTCCCACTGTATTTTGACGATCTGTTTTGCA
ATCTAATCGGCGCGGGCGAGCACGCCGGTATTCTGGACAGCTTGCTGGAACGTTTGGCGACCTACCAGGAAAAAACGCTG
GCCATCAAGAGCAAAATCAAAGGGGCGTTAATGTATCCGGTTTCAATTATTGCCATCGCCTTTATTATTACCGCAGTGAT
CATGATTTTTGTGATCCCCGCGTTTAAGCAGTTATTCTCCAGCTTTGGCGCCGATTTGCCCGCACCTACGCTCATTATGA
TGAGTATCTCCGATTTTTTCGTTGCTTATTGGTGGGCGATCTTCAGTATTATAGGAGGTGGACTTTATAGCTTTTTCTAC
TTCTGGAAACGTAACAAAAAAATGCAGGACGTCATAGATAGAATAATGCTCAAGCTACCAATTTTTGGAAATGTAATCCG
TAAAGCCTCCATAGCACGATGGACACGTACACTGGCAACCATGTTTGCCGCCGGAGTGCCACTGGTGGAGGCGCTTAACT
CGGTAGCAGGTGCTGCGGGCAACGTAGTGTATTTTGATGCGACCAAGATAATCCAGCGGGAAGTCAGCCAAGGTGGCACC
CTGACGGCAGCCATGCAAAATGTAGGGGTATTCCCCAGCATGGTGTTGCAAATGGTAGCAATCGGCGAAGAAGCAGGTTC
ACTGGATGGCATGTTAAACAAAGTGGCAGATTTTTTTGAAGCTGAGGTAGACGATGCAGTGGCAGCGTTGGCCAGCCTGA
TGGAGCCTATCATCATGGTGGTGCTAGGCACACTAATAGGCGGTATGGTGATAGCAATGTATCTGCCCATTTTCAAGATG
GGGCAAGCTGTTGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(70-193)

Type II secretion system (T2SS), protein F (T2SSF)

(274-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

50.864

100

0.509
  pilC Legionella pneumophila strain ERS1305867

51.256

98.272

0.504
  pilG Neisseria gonorrhoeae MS11

50.754

98.272

0.499
  pilG Neisseria meningitidis 44/76-A

50.376

98.519

0.496
  pilC Acinetobacter baylyi ADP1

47.677

100

0.481
  pilC Acinetobacter baumannii D1279779

48.866

98.025

0.479
  pilC Vibrio cholerae strain A1552

39.196

98.272

0.385
  pilC Vibrio campbellii strain DS40M4

37.811

99.259

0.375

Multiple sequence alignment