Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MFPB16_RS06915 Genome accession   NZ_LT960788
Coordinates   1377348..1378238 (-) Length   296 a.a.
NCBI ID   WP_099960319.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain MFPB16A1401     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1372348..1383238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MFPB16_RS06880 (MFPB16_1357) - 1373414..1374424 (-) 1011 WP_099960312.1 class I SAM-dependent methyltransferase -
  MFPB16_RS06885 (MFPB16_1358) - 1374548..1374886 (-) 339 WP_100970230.1 hypothetical protein -
  MFPB16_RS06890 (MFPB16_1359) comGF 1374849..1375361 (-) 513 WP_099960314.1 competence type IV pilus minor pilin ComGF Machinery gene
  MFPB16_RS06895 (MFPB16_1360) comGE 1375336..1375641 (-) 306 WP_099960315.1 type II secretion system protein Machinery gene
  MFPB16_RS06900 (MFPB16_1361) comGD 1375628..1376080 (-) 453 WP_099960316.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MFPB16_RS06905 (MFPB16_1362) comGC 1376052..1376351 (-) 300 WP_099960317.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MFPB16_RS06910 (MFPB16_1363) comGB 1376348..1377355 (-) 1008 WP_099960318.1 type II secretion system F family protein Machinery gene
  MFPB16_RS06915 (MFPB16_1364) comGA 1377348..1378238 (-) 891 WP_099960319.1 competence type IV pilus ATPase ComGA Machinery gene
  MFPB16_RS06920 (MFPB16_1365) - 1378353..1379084 (-) 732 WP_011375002.1 YebC/PmpR family DNA-binding transcriptional regulator -
  MFPB16_RS06925 (MFPB16_1366) - 1379175..1379675 (-) 501 WP_099960320.1 VanZ family protein -
  MFPB16_RS06930 (MFPB16_1367) - 1379772..1380146 (+) 375 WP_099960321.1 hypothetical protein -
  MFPB16_RS06940 (MFPB16_1368) - 1380333..1381733 (-) 1401 WP_099960322.1 hypothetical protein -
  MFPB16_RS06945 (MFPB16_1369) - 1381747..1382802 (-) 1056 WP_143826717.1 hypothetical protein -

Sequence

Protein


Download         Length: 296 a.a.        Molecular weight: 32985.83 Da        Isoelectric Point: 6.7524

>NTDB_id=1148478 MFPB16_RS06915 WP_099960319.1 1377348..1378238(-) (comGA) [Latilactobacillus sakei strain MFPB16A1401]
MSTEEKLTTLLKHCAKQRVQDLYFTPTIGGWRLTERRQSDLVTNQELDKPTGTRYLNRLKYMAGMDISETRRAQTGRSEL
QLGDQTIYLRLATVGDFLNRESLVIRFIYPIGAIYHCDNQQILAQLTQMSRQAGLILFAGPTGSGKTTSLYHLAQQSMSA
KMVVAIEDPIEIVAPEFLQLQVNDSAGLSYAELLKISLRLRPDTLIIGEIRDLETAQYAVSAALSGHLVLSTIHARSTQG
VVARLLDLGISEMQLRACLTAIAYQSLVTKDDVVQAHYELLTGSDYFSKKEGGWDV

Nucleotide


Download         Length: 891 bp        

>NTDB_id=1148478 MFPB16_RS06915 WP_099960319.1 1377348..1378238(-) (comGA) [Latilactobacillus sakei strain MFPB16A1401]
ATGTCAACTGAAGAAAAGCTAACAACTTTATTAAAGCATTGTGCTAAACAACGTGTTCAAGATCTTTATTTTACACCGAC
AATAGGTGGCTGGCGATTGACGGAACGTCGTCAGAGCGATTTGGTCACCAATCAAGAATTGGATAAACCGACTGGAACGC
GTTATTTAAATCGACTGAAGTATATGGCGGGGATGGATATCAGCGAGACTCGGCGTGCACAAACCGGCCGCTCGGAATTA
CAGCTAGGCGACCAAACAATTTATTTACGCCTAGCGACGGTCGGGGATTTTTTAAATCGCGAAAGCTTGGTCATTCGGTT
TATCTATCCAATTGGTGCCATCTATCATTGTGACAATCAACAGATTTTGGCGCAATTAACGCAGATGAGTCGACAGGCGG
GCTTAATTCTTTTCGCTGGGCCAACCGGTTCTGGCAAAACAACTAGTCTCTATCATCTGGCCCAACAATCAATGAGTGCC
AAGATGGTGGTTGCTATTGAAGATCCGATTGAAATTGTTGCACCTGAGTTTTTGCAACTGCAGGTTAACGATAGTGCGGG
ATTGAGCTATGCCGAGCTATTAAAAATTAGTTTACGACTGCGACCGGATACGCTGATTATCGGTGAGATTCGCGATTTAG
AGACGGCTCAATATGCGGTTTCTGCGGCCTTAAGTGGTCACTTGGTATTGAGTACTATTCACGCTCGGAGTACACAGGGC
GTGGTTGCGCGCTTATTGGATCTGGGGATTTCTGAGATGCAATTGCGGGCCTGTCTAACGGCGATTGCCTACCAGTCGTT
GGTGACAAAAGACGACGTTGTGCAAGCCCATTATGAATTATTAACTGGTTCAGATTATTTCTCAAAAAAAGAAGGTGGTT
GGGATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

97.297

100

0.973
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

39.59

98.986

0.392
  comGA/cglA/cilD Streptococcus pneumoniae D39

39.249

98.986

0.389
  comGA/cglA/cilD Streptococcus pneumoniae R6

39.249

98.986

0.389
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

39.249

98.986

0.389
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

39.249

98.986

0.389
  comGA Lactococcus lactis subsp. cremoris KW2

38.127

100

0.385
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

40.511

92.568

0.375
  comYA Streptococcus mutans UA140

36.824

100

0.368
  comYA Streptococcus mutans UA159

36.824

100

0.368
  comYA Streptococcus gordonii str. Challis substr. CH1

40.221

91.554

0.368

Multiple sequence alignment