Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CKV74_RS00415 Genome accession   NZ_LT906463
Coordinates   83702..85072 (+) Length   456 a.a.
NCBI ID   WP_007241714.1    Uniprot ID   -
Organism   Haemophilus pittmaniae strain NCTC13334     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 78702..90072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV74_RS00385 (NCTC13334_00078) - 79050..79901 (-) 852 WP_007241538.1 thymidylate synthase -
  CKV74_RS00390 (NCTC13334_00079) lgt 79914..80723 (-) 810 WP_007241655.1 prolipoprotein diacylglyceryl transferase -
  CKV74_RS00395 (NCTC13334_00080) - 80732..81526 (-) 795 WP_007241724.1 sulfite exporter TauE/SafE family protein -
  CKV74_RS00400 (NCTC13334_00081) rppH 81526..82110 (-) 585 WP_007241732.1 RNA pyrophosphohydrolase -
  CKV74_RS10615 - 82273..82407 (-) 135 WP_269457018.1 hypothetical protein -
  CKV74_RS00405 (NCTC13334_00082) ampD 82577..83125 (-) 549 WP_007241547.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CKV74_RS00410 (NCTC13334_00083) pilA 83253..83702 (+) 450 WP_007241769.1 prepilin peptidase-dependent pilin Machinery gene
  CKV74_RS00415 (NCTC13334_00084) pilB 83702..85072 (+) 1371 WP_007241714.1 GspE/PulE family protein Machinery gene
  CKV74_RS00420 (NCTC13334_00085) pilC 85072..86292 (+) 1221 WP_095176600.1 type II secretion system F family protein Machinery gene
  CKV74_RS00425 (NCTC13334_00086) - 86289..86957 (+) 669 WP_007241801.1 A24 family peptidase -
  CKV74_RS00430 (NCTC13334_00087) coaE 87018..87653 (+) 636 WP_007241821.1 dephospho-CoA kinase -
  CKV74_RS00435 (NCTC13334_00088) yacG 87646..87852 (+) 207 WP_007241731.1 DNA gyrase inhibitor YacG -
  CKV74_RS00440 (NCTC13334_00089) - 87849..88109 (+) 261 WP_095176601.1 GNAT family N-acetyltransferase -
  CKV74_RS00445 (NCTC13334_00090) cgtA 88252..89421 (-) 1170 WP_007241610.1 Obg family GTPase CgtA -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 51352.86 Da        Isoelectric Point: 6.3200

>NTDB_id=1147703 CKV74_RS00415 WP_007241714.1 83702..85072(+) (pilB) [Haemophilus pittmaniae strain NCTC13334]
MLATAQNGAVFEIAPELQQHNRQQQHLLLRYLALPLKEDQQRLWLGLDSLNNLAACETFGFLSGKLIEPVLLDSAQLKRL
LQSLIPQNKQTIETENFYQAETVSEPQIDEDEPVIVLLNQLFERALTRNASDIHLEPQPQGLQVRLRIDGVLHSEPVIAK
SLAPRVISRLKLLAKLNISETRLPQDGRFQFKTTFADILDFRLSTLPTNLGEKAVLRVQQNKPVQLQFAELGMTQSQQKQ
LRHALSQPQGLILVTGPTGSGKSITLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLRTFLRQDPD
IIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDARSAISRLQQLGIAEHEIENSLLLVIAQRLVRRLCQECHKQLATTC
ACQHGYKGRVGIYQFLHYDGRQFHTDFQDLRQSAQQKLDEGITDLAEIERILGAAA

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=1147703 CKV74_RS00415 WP_007241714.1 83702..85072(+) (pilB) [Haemophilus pittmaniae strain NCTC13334]
ATGTTAGCCACCGCCCAAAACGGCGCAGTTTTTGAAATTGCGCCGGAACTGCAACAACATAATCGGCAACAGCAACATTT
ATTGCTACGCTATTTGGCATTACCGCTCAAAGAGGATCAACAGCGCCTATGGTTAGGATTAGATTCGCTGAATAATTTGG
CCGCTTGTGAAACCTTTGGTTTTCTCAGCGGTAAATTAATCGAGCCGGTTCTGCTTGATAGCGCCCAACTTAAGCGCCTA
TTACAATCACTAATACCGCAAAATAAACAGACTATTGAAACGGAAAATTTTTATCAGGCAGAAACCGTTAGCGAACCGCA
AATCGATGAAGATGAACCGGTTATTGTGTTATTGAATCAACTGTTTGAGCGTGCGCTTACCCGAAATGCTTCGGATATTC
ACTTAGAACCTCAACCCCAAGGACTACAAGTTCGTCTGCGCATTGATGGCGTACTGCACAGCGAGCCCGTCATTGCCAAA
AGCCTCGCACCTCGGGTCATTTCTCGCTTAAAGCTATTGGCTAAACTCAACATTAGTGAAACCCGCCTACCACAAGATGG
GCGTTTTCAATTTAAAACAACCTTTGCCGATATTTTAGATTTTCGACTGTCTACGCTACCCACAAATCTGGGAGAAAAGG
CAGTATTGCGGGTACAACAAAATAAACCCGTTCAGCTTCAATTTGCCGAATTGGGAATGACACAATCACAACAAAAACAA
TTGCGTCATGCCCTTTCCCAACCGCAGGGATTAATTCTCGTCACCGGCCCCACCGGTAGCGGCAAAAGTATTACCCTCTA
CACCGCACTACAATGGTTAAATACCCCGGATAAACATATTATGACGGCGGAAGATCCCATCGAAATAGAACTAGACGGCA
TTATTCAAAGCCAAATCAATCCACAGATTGGGCTGGATTTTTCCCGCCTATTACGAACCTTTTTACGTCAGGATCCGGAC
ATTATTATGCTTGGAGAAATCCGTGACGAAGAAAGCGCAGCCATGGCATTACGCGCAGCCCAAACCGGGCATTTAGTACT
TTCAACATTACATACCAACGATGCCCGTTCAGCAATTTCCCGTCTGCAACAATTAGGTATCGCAGAGCATGAAATCGAAA
ATAGTCTGTTGTTAGTCATTGCACAGCGTTTAGTGCGACGGCTTTGCCAAGAATGCCATAAACAGCTGGCAACAACTTGT
GCCTGTCAGCATGGCTATAAAGGAAGAGTCGGAATTTATCAATTCTTACATTATGATGGGCGGCAATTTCATACAGATTT
CCAAGATTTACGCCAAAGCGCACAACAAAAATTAGATGAAGGCATTACCGACCTAGCGGAAATCGAACGTATTCTGGGAG
CTGCAGCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-387)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(20-86)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

71.145

99.561

0.708
  pilB Haemophilus influenzae Rd KW20

71.397

98.904

0.706
  pilB Glaesserella parasuis strain SC1401

57.93

99.561

0.577
  pilB Vibrio parahaemolyticus RIMD 2210633

39.01

100

0.432
  pilB Vibrio campbellii strain DS40M4

40.956

100

0.432
  pilB Vibrio cholerae strain A1552

39.959

100

0.423
  pilB Acinetobacter baumannii D1279779

39.085

100

0.412
  pilB Acinetobacter baylyi ADP1

37.55

100

0.41
  pilB Legionella pneumophila strain ERS1305867

37.014

100

0.397
  pilF Thermus thermophilus HB27

36.211

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.821

100

0.368

Multiple sequence alignment